Fig 1: Loss of MMR in stem cells elicits an apparent growth advantage(A) Western blot displaying loss of MSH2 and MSH6 expression in two independent MSH2 knockout (KO) clones created in H1 human embryonic stem cells (hESCs). Actin is a loading control.(B and C) Representative growth curves of H1 WT hESCs (B) and YK26 iPSCs (C) and corresponding MSH2 KO clones.(D) Representative growth curve comparing an MSH2 KO clone and a version in which both MSH2 alleles were restored to the WT sequence (KI).(E) Representative growth curve comparing an MLH1 KO clone with H1 WT hESCs.Each experiment in (B)–(E) was performed in triplicate with 3 independent repeats. Two-tailed Student’s t test was used to determine p values.