Fig 1: Interactions between individual human MCM subunits and HsCdt1 or HsCdc6, and effects of HsGeminin on the interactions between MCM subunits and HsCdt1. A–D, extracts of Sf9 cells expressing untagged human Mcm4 (A), Mcm5 (B), Mcm6 (C), or Mcm7 (D) were incubated at 4 °C for 3 h with extracts from control cells or extract from cells expressing FLAG-HsCdt1 or Myc-HsCdc6. For each Mcm subunit, an equal amount of protein was added in each reaction. The proteins precipitated by anti-FLAG- or anti-Myc-agarose beads were analyzed by Western blotting. L indicates 8.3% of the load. E–H, extracts of Sf9 cells expressing untagged human Mcm3 (G), Mcm5 (H), Mcm6 (E), or Mcm7 (F) were incubated at 4 °C for 3 h with extracts from control cells or extracts from cells expressing FLAG-HsCdt1 and/or FLAG-HsGeminin-His6. The proteins precipitated by anti-FLAG-agarose beads were analyzed by Western blotting. IP, immunoprecipitated.