Fig 2: SMYD3 knockdown downregulates HR gene expressions. (a) The confirmation of the microarray analyses for the expressions of candidate genes in the shSMYD3/shLuc dataset using qRT-PCR. The fold changes of each gene expression in the microarray data were listed below. *P < 0.05, **P < 0.01, ***P < 0.001 vs. shLuc control. (b) Western blot analyses of SMYD3, MDC1, EXO1 and RAD54B in shLuc and shSMYD3 MCF7 cells. Tubulin was used as a loading control. The ratio of individual protein relative to the loading control tubulin was indicated below. (c,d) DNA repair foci formation of MDC1 foci (c) and BRCA1 foci (d) formation at indicated times after 1.67 Gy IR treatment in shLuc or shSMYD3 MCF7 cells. *P < 0.05. **P < 0.01. ***P < 0.001. All values in the histograms were means ± SD of triplicates and data were representative of n = 3 for each experiment.