PE/Cyanine7 anti-human CD4 357409 from BioLegend

Product Specs
ItemPE/Cyanine7 anti-human CD4
CompanyBioLegend
Price $153.00Supplier Page View Company Product Page
Catalog Number357409
Quantity25 tests
ApplicationsFC - Quality tested
Conjugate/TagPE/Cyanine7
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ReactivityHuman
IsotypeRat IgG2b, kappa
CloneA161A1
HostRat
Antibody TypePrimary
ImmunogenHuman CD4 T cells
NCBI Full Gene NameCD4 molecule
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
ClonalityMonoclonal
TargetCD4
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Imaging CRISPR-edited CAR-T cell therapies with optical and positron emission tomography reportersTheranosticsJanuary 1, 2026Rafael Enrique Sanchez-Pupo, John Joseph Kelly, Nourhan Shalaby, Ying Xia, Francisco Manuel Martinez-Santiesteban, Jasmine Lau, Ivy Elizabeth Verriet, Matthew Stefan Fox, Justin Wesley Hicks, Jonathan Dale Thiessen, et al.Cat# 306719). CD4 and CD8 expression were evaluated using anti-human CD4 PE/Cyanine7 (BioLegend, Cat# 357409) and anti-human CD8a APC (BioLegend, Cat# 300911). SYTOX™ Blue (Thermo Fisher Scientific) was used as

(2) Proteomic analysis reveals dysregulation of peripheral blood neutrophils in patients with Multiple SclerosisClinical and Experimental ImmunologyJanuary 21, 2025Katie J Smith, Zachary Lim, Sonja Vermeren, Veronique E Miron, Sarah Dimeloe, Donald J Davidson, Anna Williams, Emily Gwyer Findlayused include: CD3 FITC (clone HIT3a, Biolegend, #300305, 1:200), CD4 PeCy7 (clone A161A1, Biolegend, #357409, 1:200), CD8 APC (clone SK1, Biolegend, #344721, 1:200), CD62L PeDazzle (clone DREG-56, Biolegend, #304805,

(3) Visualizing cell–cell communication using synthetic notch activated MRIProceedings of the National Academy of SciencesMarch 14, 2023TianDuo Wang, Yuanxin Chen, Nivin N. Nystrom, Shirley Liu, Yanghao Fu, Francisco M. Martinez, Timothy J. Scholl, John A. Ronaldsurface markers, T cells were stained for human T cell receptor (TCR, #306720), CD3 (#317335), CD4 (#357409), and CD8 (#300912) using fluorescently tagged antibodies (Biolegend), and flow cytometry was performed

(4) Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22ImmunityJanuary 10, 2023Giannou AD, Kempski J, Shiri AM, Lücke J, Zhang T, Zhao L, Zazara DE, Cortesi F, Riecken K, Amezcua Vesely MC, et al.

(5) Lkb1 aggravates diffuse large B-cell lymphoma by promoting the function of Treg cells and immune escapeJournal of translational medicineAugust 19, 2022Su X, Sun T, Li M, Xia Y, Li M, Wang D, Lu F, Ye J, Ji C.phosphate-buffered saline containing 2% FBS and were incubated with CD3 (Biolegend, 300318), CD4 (Biolegend, 357410), CD8 (Biolegend, 344703), CD25 (Biolegend, 302610), CD45RA (Biolegend, 304148), CD127 (Biolegend, 351304)

(6) Single-cell glycomics analysis by CyTOF-Lec reveals glycan features defining cells differentially susceptible to HIVeLifeJuly 5, 2022Ma T, McGregor M, Giron L, Xie G, George AF, Abdel-Mohsen M, Roan NR.344818,FACS(1:100) Antibody,"PE/Cyanine7 anti-human CD4 Antibody(Rat, Monoclonal)",Biolegend,Cat# 357410,FACS(1:100) Antibody,"APC anti-human CD8 Antibody(Mouse, Monoclonal)",Biolegend,Cat# 344722,FACS(1:100) Antibody,"FITC Rat Anti-Mouse CD24

(7) Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccineNature communicationsJune 16, 2022Kramer KJ, Wilfong EM, Voss K, Barone SM, Shiakolas AR, Raju N, Roe CE, Suryadevara N, Walker LM, Wall SC, et al.1:50), CCR7 PE (Biolegend 353204, 1:200), CD38 PerCP (Biolegend 303520, 1:100), CD4 PECy7 (Biolegend 357410, 1:100), and CD3 APCCy7 (Biolegend 300318, 1:200). Tfh markers consisted of CXCR5 APC (Biolegend 356907

(8) The transcription factor RFX5 coordinates antigen-presenting function and resistance to nutrient stress in synovial macrophagesNature metabolismJune 1, 2022Hu Z, Zhao TV, Huang T, Ohtsuki S, Jin K, Goronzy IN, Wu B, Abdel MP, Bettencourt JW, Berry GJ, et al.

(9) T cells expressing CD5/CD7 bispecific chimeric antigen receptors with fully human heavy-chain-only domains mitigate tumor antigen escapeSignal transduction and targeted therapyMarch 25, 2022Dai Z, Mu W, Zhao Y, Cheng J, Lin H, Ouyang K, Jia X, Liu J, Wei Q, Wang M, et al.

(10) Protective immune trajectories in early viral containment of non-pneumonic SARS-CoV-2 infectionNature communicationsFebruary 23, 2022Pekayvaz K, Leunig A, Kaiser R, Joppich M, Brambs S, Janjic A, Popp O, Nixdorf D, Fumagalli V, Schmidt N, et al.CD56 (BioLegend #362512). To sort T cells, we used PE CD3 (BioLegend #300308), PE-Cy7 CD4 (BioLegend #357410), AF488 CD8a (BioLegend #301024), and APC-Cy7 CD19 (BioLegend #363010). Nasal swab preparation For

(11) Hyaluronic acid is a negative regulator of mucosal fibroblast-mediated enhancement of HIV infectionMucosal immunologySeptember 1, 2021Egedal JH, Xie G, Packard TA, Laustsen A, Neidleman J, Georgiou K, Pillai SK, Greene WC, Jakobsen MR, Roan NR.consisted of APC/Cy7-conjugated antiCD3 (Biolegend, #344818), PE/Cy7-conjugated anti-CD4 (Biolegend #357410) and APC-conjugated anti-CD8 (Biolegend, #344722). Stained cells were washed in FACS buffer and ﬁxed

(12) GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancerJournal for immunotherapy of cancerJuly 1, 2021Wei JL, Wu SY, Yang YS, Xiao Y, Jin X, Xu XE, Hu X, Li DQ, Jiang YZ, Shao ZM.#100708), mouse PD-1 (BioLegend, Cat#135210), mouse CD25 (BioLegend, Cat#101912), human CD4 (BioLegend, Cat#357409), human CD25 (BioLegend, Cat#302625), and human PD-1 (BioLegend, Cat#329908). Intracellular staining

(13) Single-cell transcriptomic analysis reveals disparate effector differentiation pathways in human T_reg compartmentNature communicationsJune 23, 2021Luo Y, Xu C, Wang B, Niu Q, Su X, Bai Y, Zhu S, Zhao C, Sun Y, Wang J, et al.

(14) Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applicationsSTAR protocolsJune 18, 2021Hearnden R, Sandhar B, Vyas V, Longhi MP.

(15) Potent ex vivo armed T cells using recombinant bispecific antibodies for adoptive immunotherapy with reduced cytokine releaseJournal for immunotherapy of cancerMay 1, 2021Park JA, Santich BH, Xu H, Lum LG, Cheung NV.expression using antibodies against human CD3 (BioLegend, Cat# 300308, RRID:AB_314044), CD4 (BioLegend, Cat# 357410, RRID:AB_2565662), CD8 (BioLegend, Cat# 300912, RRID:AB_314116), γδ T cell receptor (TCR) (BioLegend,

(16) The impact of cell type and context-dependent regulatory variants on human immune traitsGenome biologyApril 29, 2021Mu Z, Wei W, Fair B, Miao J, Zhu P, Li YI.following antibodies in PBS for 30 min: anti-CD3-APC/Cy7 (Biolegend, 300426), anti-CD4-PE/Cy7 (Biolegend, 357410), anti-CD8-Percp/Cy5.5 (Biolegend, 301032), anti-CD25-PE/CF594(BD Horizon,562525), anti-CD19-FITC (Biolegend,302206),

(17) The Outcome of Neutrophil-T Cell Contact Differs Depending on Activation Status of Both Cell TypesFrontiers in immunologyJanuary 1, 2021Minns D, Smith KJ, Hardisty G, Rossi AG, Gwyer Findlay E.Fortessa cytometer (BD Biosciences) and FlowJo software. Antibodies CD4-PE/Cy7 (clone A161A1, Biolegend, #357410, lot B225074); CD8-AF647 (clone HIT8a, Biolegend, #300918, lot B235677); PD1-PE (clone EH12.2A7, Biolegend,

(18) GD2 or HER2 targeting T cell engaging bispecific antibodies to treat osteosarcomaJournal of hematology & oncologyDecember 10, 2020Park JA, Cheung NV.cytometry. Anti-human antibodies against CD3 (BioLegend, Cat# 300308, RRID:AB_314044), CD4 (BioLegend, Cat# 357410, RRID:AB_2565662), CD8 (BioLegend, Cat# 300912, RRID:AB_314116), and CD45 (BioLegend, Cat# 304012, RRID:AB_314400) were

(19) CD147-spike protein is a novel route for SARS-CoV-2 infection to host cellsSignal transduction and targeted therapyDecember 4, 2020Wang K, Chen W, Zhang Z, Deng Y, Lian JQ, Du P, Wei D, Zhang Y, Sun XX, Gong L, et al.APC/ Cyanine7 anti-human CD3 antibody (300426, Biolegend, USA), PE/ Cyanine7 anti-human CD4 antibody (357410, Biolegend, USA), and PerCP anti-human CD8a antibody (300922, Biolegend, USA). 1 × 106 CD4+ or CD8+ T cells

(20) Dynamic Cardiolipin Synthesis Is Required for CD8⁺ T Cell ImmunityCell metabolismDecember 1, 2020Corrado M, Edwards-Hicks J, Villa M, Flachsmann LJ, Sanin DE, Jacobs M, Baixauli F, Stanczak M, Anderson E, Azuma M, et al.AB_2100370 BV711 anti-human CD28,Biolegend,Cat#302947; RRID: AB_2616856 PE-Cy7 anti-human CD4,Biolegend,Cat#357409; RRID: AB_2565661 BV711 anti-human CD25,Biolegend,Cat#302635; RRID: AB_11219793 AF647 anti-human IFNg,Biolegend,Cat#502516;

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PE/Cyanine7 anti-human CD4 from BioLegend

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PE/Cyanine7 anti-human CD4 from BioLegend

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