Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb 4199T from Cell Signaling Technology

Published Figures

Register or Sign In to View Figures.

EMBO reports
December 05 2019

Fig 1: CASP4/5 mediate MRSA‐induced CASP1 activation and IL‐1β release in human THP‐1 macrophages Immunoblot analysis of cleaved IL‐1β and CASP1 (p20) in supernatants from Cas9 control, CASP4 −/−, CASP5 −/−, CASP4/5 −/−, and CASP1 −/− THP‐1 cells infected with MRSA at 24 h post‐infection. Densitometry analysis represents mean ± SEM (n = 4 biological replicates). Statistical analysis was performed using two‐way ANOVA. **P ≤ 0.01, ***P ≤ 0.001, NT = no treatment.Immunoblot analysis of pro‐CASP1 (p45) in lysates from Cas9 control, CASP4 −/−, CASP5 −/−, CASP4/5 −/−, and CASP1 −/− THP‐1 cells infected with MRSA at 24 h post‐infection. Densitometry analysis represents mean ± SEM (n = 5 biological replicates). Statistical analysis was performed using two‐way ANOVA. *P ≤ 0.05, ***P ≤ 0.001, NT = no treatment.MRSA‐induced LDH released in supernatants from Cas9 control, CASP4 −/−, CASP5 −/−, CASP4/5 −/−, and CASP1 −/− THP‐1 cells at 24 h post‐infection. Data represent mean ± SEM (n = 15 biological replicates). Statistical analysis was performed using one‐way ANOVA. *P ≤ 0.05, ***P ≤ 0.001.Cytokine release from Cas9 control, CASP4 −/−, CASP5 −/−, CASP4/5 −/−, and CASP1 −/− THP‐1 cells infected with MRSA at 24 h post‐infection. Data represent mean ± SEM (n = 10 biological replicates). Statistical analysis was performed using one‐way ANOVA. *P ≤ 0.05, **P ≤ 0.01.
Register or Sign In to View Figures.

Cells
April 20 2022

Fig 2: Effect of cannabinoids on the expression of NLRP3 inflammasome proteins in THP-1 macrophages. (A): CBD and THC decreased the expression of NLRP3 after LPS + ATP stimulation in THP-1 macrophages: Representative Western blots of NLRP3 and GAPDH bands and the quantification of the same using one-way ANOVA with Tukey’s multiple-comparison test. Four independent experiments (n = 3–4) of THP-1 cell lysates obtained after pre-treatment with CBD or THC for 30 min and LPS stimulation for 3 h followed by ATP for 1 h were analyzed, and data are expressed as means ± SD; * p < 0.05, ** p < 0.01. (Abbreviation: LA: LPS + ATP). (B): CBD and THC decreased the ratio of the expressions of caspase-1 p20/p45 after LPS + ATP stimulation in THP-1 macrophages: Representative Western blots of caspase-1 p45 (pro-caspase1), p20, and GAPDH bands and the quantification of caspase-1 p20/p45 ratio using one-way ANOVA with Tukey’s multiple-comparison test. Four independent experiments (n = 4) of THP-1 cell lysates obtained after pre-treatment with CBD or THC for 30 min and LPS stimulation for 3 h followed by ATP for 1 h were analyzed, and data are expressed as means ± SD; * p < 0.05, ** p < 0.01. (Abbreviation: LA: LPS + ATP). (C): CBD and THC decreased the expression of pro-IL-1β after LPS + ATP stimulation in THP-1 macrophages: Representative Western blots of pro-IL-1β and GAPDH bands and the quantification of the same using one-way ANOVA with Tukey’s multiple-comparison test. Four independent experiments (n = 4) of THP-1 cell lysates obtained after pre-treatment with CBD or THC for 30 min and LPS stimulation for 3 h followed by ATP for 1 h were analyzed, and data are expressed as means ± SD; ** p < 0.01, *** p < 0.001. (Abbreviation: LA: LPS + ATP). (D): CBD and THC decreased the expression of mature IL-1β after LPS + ATP stimulation in cell supernatants of THP-1 macrophages: Representative Western blots of mature-IL-1β and β-actin bands and the quantification of the same using one-way ANOVA with Tukey’s multiple-comparison test. Four independent experiments (n = 4) of THP-1 cell supernatants obtained after pre-treatment with CBD or THC for 30 min and LPS stimulation for 3 h followed by ATP for 1 h were analyzed, and data are expressed as means ± SD; ** p < 0.01, *** p < 0.001, **** p < 0.0001. (Abbreviation: LA: LPS + ATP).
Register or Sign In to View Figures.

Biomedicines
February 24 2022

Fig 3: SUCNR1 and inflammasome components are increased and positively correlated in surgical resections from UC patients. (a) Graphs show mRNA expression of SUCNR1, NLPR3, CASP-1, IL1B, IL18 and ASC in intestinal resections from UC patients (n = 25) and non-IBD patients (n = 30). (b) Graphs show protein expression of SUCNR1, NLRP3 and Caspase-1/pro-Caspase-1 (n = 15). Bars in graphs represent mean ± SEM. * p < 0.05 vs. non-IBD patients. (c) Graphs show the correlations between data relative to mRNA expression of SUCNR1 vs. mRNA expression of the inflammasome components NLRP3, CASP1, IL1B and IL18 (expressed as ΔCt). In each correlation, the value of the Spearman’s correlation coefficient (R) and p value are shown.
Register or Sign In to View Figures.

Biomedicines
February 24 2022

Fig 4: SUCNR1 mediates inflammasome priming in intestinal epithelial cells. (a) HT29 cells treated with succinate 1 mM and/or an inflammatory cocktail. Graphs show mRNA (n = 7) and protein (n = 3) expression of SUCNR1. Image of a representative Western Blot of one independent experiment. Bars in graphs represent mean ± SEM; * p < 0.05 and ** p < 0.01 vs. vehicle cells. (b–e) HT29 cells transiently transfected with a specific siRNA against SUCNR1 or ctrl; (b) Graphs show mRNA and protein expression of SUCNR1 (n = 5). (c) Graphs show mRNA expression of NLRP3, CASP1, IL1B, ASC and IL18 (n = 5). (d) Graph shows protein expression of pro-Caspase-1 (n = 4). Image of a representative Western Blot of one independent experiment. (e) Graph shows secreted protein levels of IL-1β detected in the supernatant of HT29 cells (n = 3). (f) Graph shows succinate levels in supernatant of HT29 cells (n = 6). In all cases, bars in graphs represent mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. siCtrl vehicle cells. # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. the respective siCtrl cells. $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 vs. siCtrl cocktail treated cells.
Register or Sign In to View Figures.

Cell death & disease
September 16 2021

Fig 5: High expression of PRMT5 and low expression of CASP1 associate with poor clinical outcomes in MM patients.A, B Representative images of IHC staining of PRMT5 and CASP1 in MM bone marrow tissues, which were divided into a high-expression group (upper panel) and a low-expression group (lower panel). Scale bar = 50 μm. C Overall survival of high PRMT5 (n = 78) and low PRMT5 (n = 30) MM patients performed by Kaplan-Meier survival analysis. D Overall survival of high CASP1 (n = 39) and low CASP1 (n = 69) MM patients performed by Kaplan-Meier survival analysis. E Kaplan-Meier survival analysis of MM patients based on both PRMT5 and CASP1 expression. MM patients were divided into four groups: high PRMT5 and low CASP1 expression (n = 51), high PRMT5 and high CASP1 expression (n = 27), low PRMT5 and high CASP1 expression (n = 12), and low PRMT5 and low CASP1 expression (n = 18). P-values were calculated using the group of high PRMT5 and low CASP1 expression as the reference group. F A model for PRMT5-dependent cell proliferation and apoptosis by silencing CASP1 in MM.

Be the first to write a review!

Citations:

(255)

Figures:

$163.00 Inquire

from
Cell Signaling Technology for
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb

The Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody. This antibody recognizes Homo Sapiens, and Human antigen. The Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb Antibody has been shown to work in the following applications: Precipitation, Immunoprecipitation, and Western Blot.

Description

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb detects endogenous levels of the p20 subunit of human caspase-1 only upon cleavage at Asp297

Product Specs
ItemCleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb
CompanyCell Signaling Technology
Price $163.00 Inquire
Catalog Number4199T
Quantity20 µl
ApplicationsW, IP
ReactivityH
TargetCasp1 (p20) cleaved
IsotypeIgG
HostRabbit
NCBI Full Gene Namecaspase 1
NCBI Gene AliasesICE, IL1BC, P45
ClonalityMonoclonal
Research AreaApoptosis
Molecular Weight20, 22

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Stress Hyperglycemia Drives CD4+ T Cell PANoptosis and Postoperative Organ Injury via Monocyte-Derived SuccinateAdv Sci (Weinh)August 5, 2026Shuai Zhao, Xuemei Miao, Wei Luo, Tao Liu, Yang Wang, Jiaying Li, Di Fu, Weiyun Shen, Yan Luo, Kang Chen, et al.1:2000), caspase‐1 (Proteintech, 84735‐1‐RR, 1:2000), cleaved caspase‐1 (Cell Signaling Technology, #4199, 1:1000), GSDMD‐N (Abcam, ab215203, 1:1000), GSDMD (Abcam, ab219800, 1:2000), MLKL (Cell Signaling Technology,

(2) Inhibiting HIF-1α-Induced miR-663b Improves Pulmonary Arterial Hypertension by Reducing Dysfunctions of PASMCs Through Repressing Sirt1-ZBP1-Mediated PANoptosisJ Cell Mol MedAugust 3, 2026Le Shen, Honghong Ma, Yang Yu, Yan Xu, Qun Yuan, Xia Zhao, Bing Zhuan1:500), anti‐NLRP3 (ab263899, Abcam, 1:1000), anti‐ASC (ab307560, Abcam, 1:1000), anti‐cleaved‐Casp1 (#4199, Cell Signalling Technology, 1:1000), anti‐GSDMD (ab219800, Abcam, 1:1000), anti‐Bax (ab32503, Abcam,

(3) Protocol to study inflammasome activation in induced pluripotent stem cell-derived macrophagesSTAR ProtocJuly 28, 2026Chloe M. McKee, Melanie Cranston, Emma C. McKay, Mohammad Arefian, Ben C. Collins, Rebecca C. Coll,Cell Signaling Technology,3866; RRID:AB_2069051 Anti-cleaved caspase-1 (1:1000),Cell Signaling Technology,4199S; RRID:AB_1903916 Anti-GSDMD N-terminal (1:1000),Abcam,ab215203; RRID:AB_2916166 Anti-GSDMD C-terminal

(4) Rewiring Pyroptosis to Potentiate Cancer Immunotherapy via a Gasdermin D Agonist Bypassing Caspase-3Adv Sci (Weinh)July 23, 2026Dan Zhao, Lingling Zhang, Yue Wen, Lianghui Cheng, Sidan Tian, Fanling Meng, Liang LuoCell Signaling Technology, 9496T), Casp‐1 (1:1000, Affinity, AF5418), Cl‐Casp‐1 (1:1000, Cell Signaling Technology, 4199T; 1:1000, Affinity, AF4005), GSDMD (1:1000, Affinity, AF4012), GSDME‐N (1:1000, Affinity, AF4016), and

(5) Epigenetic-metabolic coupling by SOX3/WDR5-SIRT5 signaling mediates the therapeutic effects of TGFβ-targeted exosomal nanoparticles in osteoarthritisMater Today BioJuly 21, 2026Fu Qiwei, Shao Jiahua, Chen Shu, Cao Jia, Li Haobo, Zhu Junanti-H3K4me3 (CST, #9751, 1:1000), anti-GSDMD-N (Abcam, ab215203, 1:1000), anti-cleaved caspase-1 (p20) (CST, #4199, 1:1000), anti-FTH1 (CST, #3998, 1:1000), anti-GPX4 (Abcam, ab125066, 1:1000), anti-ACSL4 (Abcam, ab155282,

(6) Linear ubiquitin chain assembly complex contributes to NLRP3-mediated pyroptotic cell deathiScienceJuly 17, 2026Tiphaine Douanne, Rosalie Moreau, Valeria Trapani, Kilian Trillet, Hubert B. Leloup, Virginie Petrilli, Julie Gavard, Nicolas Bidère(HAP46.5, sc-56698), GAPDH (6C5, sc-32233), IL-1β (AS57, sc-52771). Antibodies to cleaved CASP1 (D57A2, 4199), GSDMD (E9S1X, 39754), OTULIN (14127), and NLRP3 (D4D8T, 15101), cleaved IL-1β (Asp116, D3A3Z, 83186)

(7) Impaired NLRP3 inflammasome activation by chitin underlies refractory chromoblastomycosis caused by Fonsecaea pedrosoi muriform cellsFront ImmunolJuly 14, 2026Yao Chen, Zilu Qu, Zhaolan Xie, Xiaowen Wang, Zhongsheng Tong, Luoyao Yang, Xu Zhang, Liuqing Chen, Bilin Dong(1:5000, #87271-4-RR, Proteintech); rabbit anti-cleaved-caspase-1 (p20) monoclonal antibody (1:1000, #4199, CST), and rabbit anti-β-actin monoclonal antibody (1:1000, #4970, Cell Signaling). Following washes

(8) Combination of polygonatum sibiricum and hedyotis diffusa induces pyroptosis in non-small cell lung cancer cells via the TLR4/NLRP3/Caspase-1/GSDMD pathway: an in vitro and in vivo study using medicated serum and herbal extractFront PharmacolJuly 6, 2026Lei Mao, Lingying Jiang, Ye Zhou, Shouhang Xu, Tingting Lou, Weiyong Lu, Gaoyuan Zhu, Jianwu Chenpurchased from Abcam. TLR4 (Batch No.: 48-2300) was purchased from Thermo Fisher. Caspase-1 (Batch No.: 4199) and GSDMD (Batch No.: 36425) were purchased from Cell Signaling Technology. The Ki67 antibody was purchased

(9) Seawater Immersion Hypothermia Triggers Cardiac Pyroptosis via the NF-κB/NLRP3 Inflammasome Axis: A Mechanistic Study in RatsInternational Journal of Molecular SciencesJune 30, 2026Huifang Deng, Chaoyue Sun, Zhibo Wang, Hongbiao Chen, Yiwen Ben, Yukun Wu, Wumu Xu, Jiaqi Wang, Yajing Wang, Yanrong Gong, et al.indicated dilutions: NLRP3 (PT0049R, Immunoway, San Jose, CA, USA, 1:1000), Cleaved-Caspase-1 (Asp297) (#4199T, Cell Signaling Technology, Danvers, MA, USA, 1:1000), Cleaved-IL-1β (Asp117) (#63124, Cell Signaling Technology

(10) Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signalingNeurobiology of StressJune 1, 2026Rui Xu, Chi Zhang, Ke Chen, Yue Shi, Chun-Rong Liang, Chun-Yan Long, Hui-Yun Li, Cheng-Kang He, Ling LiAbcam), KEAP1 (8047, Cell Signaling Technology), NLRP3 (AG-20B-0014, AdipoGen), cleaved caspase-1 (4199, Cell Signaling Technology), cleaved IL-1β (83186, Cell Signaling Technology), and β-actin (A1978, Sigma-Aldrich).

(11) Cell autonomous inflammation in VEXAS is mediated by cGAS-STINGbioRxivMay 29, 2026Samuel J. Magaziner, Jason C. Collins, Brecca Miller, Patrick Zheng, Amy K. Wang, Jerome Hadjadj, Juan Carlos Baladrán, Maria Sirenko, Maya English, James Bertlin, et al.pro-IL-1β (Cell Signaling, 12242S), Caspase 1 (Cell Signaling, 3866T), Cleaved Caspase 1 (Cell Signaling, 4199T), Caspase 3 (Cell Signaling, 14220S), Cleaved Caspase 3 (Cell Signaling, 9661S), Caspase 8 (Cell Signaling,

(12) SETD7-mediated epigenetic regulation of LncRNA XIST promotes trophoblast pyroptosis: a study in preeclampsia mouse and cell modelsBiology DirectMay 23, 2026Xiaolan Zhao, Lingling Li, Yujue Wang, Zhenrong ZhengWuhan, Hubei, China), NLRP3 (1:2000, 30109-1-AP, Proteintech), cleaved Caspase-1 (1:1000, #89332 / #4199, Cell Signaling Technology, Danvers, MA, USA), Gasdermin D N-terminal fragment (GSDMD-N) (1:5000, ab215203,

(13) Pharmacological targeting of the NLRP3 LRR domain with isothiazolinones overcomes CRID3-resistant inflammationEMBO Molecular MedicineApril 17, 2026Hawon Woo, Yeonseo Jang, Soyeon Kim, Wonyoung Kim, Fenfen Zhang, Raghvendra Mall, Chirag N Patel, Melan Kurera, Chinh Ngo, Simon H Jiang, et al.primary antibodies: caspase-1 (AdipoGen, AG-20B-0042, 1:1000), human cleaved caspase-1 (Cell Signaling, 4199, 1:1000), human caspase-1 (Cell Signaling, 2225, 1:1000), caspase-3 (Cell Signaling, 9662, 1:1000),

(14) Extracellular caspase-1: a critical inducer and a therapeutic target of lung injury in gut ischemia-reperfusionFront ImmunolApril 15, 2026Kouhei Ishikawa, Gaifeng Ma, Alok Jha, Atsushi Murao, Ping Wang, Monowar Azizaccording to the manufacturers’ instructions: anti-cleaved caspase-1 (Asp297) (p20 subunit; Cat. No. 4199S, 1:1000) and anti-cleaved caspase-1 (Asp296) (p20 subunit; Cat. No. 89332S, 1:1000) (all from Cell Signaling Technology

(15) Hematopoietic cell kinase regulates microglial/macrophage activation to drive neuroinflammation after traumatic brain injuryCell Communication and SignalingApril 9, 2026Lei Li, Taoyuan Lu, Weiwei Gao, Fanjian Li, Xin Guo, Siyu Guan, Dilmurat Gheyret, Jinchao Wang, Tuo Li, Yiyao Cao, et al.NLRP3 (Cat# 19771-1-AP; Proteintech), cst ASC (CAT# ab309497; Abcam), cleaved (CL)-Caspase-1 (Cat# 4199; CST), IL-1β (Cat# ab283822; Abcam), and β-actin (Cat# 4967; CST). After washing, membranes were incubated

(16) Specific inhibition of NLRP3 inflammasome by a Smurf1 inhibitor in vitro and in vivoOpen Med (Wars)April 9, 2026Lifeng Meng, Hongmei Xuan, Ping Zhou, Weifeng Xu, Wei Bian, Yunxiao Lin, Ying GuanAG-20B-0042) and anti-NLRP3 (1:2000, AG-20B-0014) were purchased from Adipogen. Anti-human caspase-1 (1:2000, 4199S) was purchased from Cell Signaling Technology. Anti-ASC (1:1000, sc-22514-R) were purchased from Santa Cruz

(17) Statins targeting mevalonate-geranylgeranyl diphosphate pathway inhibit proliferation in NK/T lymphoma by promoting pyroptosisTransl OncolApril 2, 2026Yingjun Wang, Mei Mei, Jieting Wang, Lai Wei, Mengqian Zhang, Mingzhi Zhangprimary antibodies against pro-caspase-1 (1:1000, ab179515, Abcam, England), cleaved caspase-1 (1:1000, 4199, CST, USA), GSDMD (1:2000, 20770-1-AP, Proteintech, Wuhan, China), cleaved N-terminal GSDMD (1:1000,

(18) Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial CellsJ Inflamm ResMarch 7, 2026Fan He, Chunzi Liu, Yun Yu, Qiaofang Wang, Lin Li, Xiaoyun Chao, Liping Chen, Cong Yudescribed.23 The following primary antibodies were used: anti-cleaved caspase-1 (1:1000; Cat. No. #4199; Cell Signaling Technology, MA, USA), anti-MT2 (1:1000; Cat. No. ab203346; Abcam, Cambridge, UK), anti-ETS1

(19) Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial CellsJ Inflamm ResMarch 7, 2026Fan He, Chunzi Liu, Yun Yu, Qiaofang Wang, Lin Li, Xiaoyun Chao, Liping Chen, Cong Yudescribed.23 The following primary antibodies were used: anti-cleaved caspase-1 (1:1000; Cat. No. #4199; Cell Signaling Technology, MA, USA), anti-MT2 (1:1000; Cat. No. ab203346; Abcam, Cambridge, UK), anti-ETS1

(20) STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degenerationCell Death & DiseaseFebruary 27, 2026Chao Huang, Vishnu Suresh Babu, Sridhar Bammidi, Jakob N. Arnold, Martin Ebeling, Gabriella Widmer, Pamela Strassburger, Mirjana Lazendic, Sabine Grüner, Janis Koester, et al.Cell Signaling Technology, Cat. #4904), anti-cleaved caspase 1 (1:1000, Cell Signaling Technology, Cat. #4199), anti-H3 (1:2000, Cell Signaling Technology, Cat. #4499, Cell Signaling Technology), anti-β-Actin (1:2000,

Be the First to Write a Review!

EMBO reports

Cells

Biomedicines

Biomedicines

Cell death & disease

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb from Cell Signaling Technology

Description

Cell Signaling Technology

Citations (255)

Reviews

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map