Fig 1: H-120 induces DNA damage in HEL cells and arrest in G2/M phase. (A) Cell cycle arrest evaluation by flow cytometry after 24 h, and densitometry plots depicting the variations in the stages of cell cycle arrest. (B) The expression of CDC25C, p-CDC25C, CyclinB1, and p53 was measured by Western blotting. (C) Quantification of relative protein expression levels of CDC25C, p-CDC25C, CyclinB1, and p53, with β-actin used as loading control. (D) Photomicrograph of DNA damage (the pointing of the arrow) at indicated concentrations of H-120 treatment (magnification, ×200; scale bar = 100 μm). Data are represented as mean ± SD (n = 3, * p < 0.05, ** p < 0.01, *** p <0.001, vs. the DMSO group).