Fig 1: Expression levels of pRb protein and E2F genes in HDPCs.(A) pRb was labeled with FITC (green) in cells, and nuclei were labeled with DAPI (blue). Scale bar = 125 μm. (B, C) The pRb and p-pRb protein level was determined by immunoblotting assay. The relative band intensities were evaluated by ImageJ software. Data are presented as the mean SD. **p < 0.01 by t-test. (D) The gene expression levels of E2F1-3 were determined. The relative band intensities were evaluated by ImageJ software. Data are reported as the mean SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 by t-test.