Fig 2: Regulation of Gene Expression by Dietary Se(A) Principal components ofthe global transcriptome of liver and brain of mice fed 0, 0.1, 0.4, or 2.25 ppm Se diets. Each point represents one mouse and color indicates Se dietary groups.(B) Selenoprotein gene expression in liver and brain. The y axis indicates the total read counts for the indicated transcripts. Positive values indicate that there are more reads in the liver than in the brain, and negative values that there are more reads in the brain than in the liver.(C) Volcano plot of transcripts affected by Se deficiency (comparison between 0 and 0.1 ppm Se diets). Each dot corresponds to a single transcript, and pink dots are significant in both -log p values (y axis) and fold change (x axis). Right: this corresponds to transcripts decreased by Se deficiency. Left: those increased by Se deficiency.(D) Network analysis of enriched pathways and interactions. A total of 81 transcripts with lower expression in response to Se deficiency (based on -log p value) were analyzed and visualized with STRING (https://string-db.org). Highly clustered transcripts circled in red correspond to biosynthesis of amino acids (FDR, 4.27E–06).(E) Western blot analyses of the livers of mice subjected to the indicated Se diets (0, 0.1, 0.4, and 2.25 ppm). Proteins probed are indicated on the right. Ulk1 is highly phosphorylated by Se deficiency.