Fig 2: GSEA of KEGG pathway gene sets in IFI16 (A), LMNB1 (B), RHBDF2 (C), and TACC3 (D) high versus low samples from TCGA database. The normalized enrichment scores (NES) are shown in each plot.

Fig 3: The landscape of immune infiltration in TCGA ccRCC patients. (A) The abundance fraction of 22 tumor-infiltrating lymphocytes (TILs) in the 396 ccRCC samples. Each column represents a sample, and each column with a different color and height indicates the abundance fraction of TILs in that sample. (B) The correlation between the abundance fractions of various immune cells. The value represents the correlation value. Red represents a positive correlation, and blue represents a negative correlation. (C) The relationship between expression of IFI16, LMNB1, RHBDF2 and TACC3 and various TILs.

Fig 4: Kaplan-Meier survival analysis showing the higher expressions of IFI16 (A), LMNB1 (B), RHBDF2 (C), and TACC3 (D) that were correlated with poor survival of ccRCC patients in the TCGA database.

Fig 5: The expression of these Hub genes in human ccRCC specimens and adjacent normal tissues (ANT). (A–D) q-RTPCR analysis of IFI16 (A), TACC3 (B), LMNB1 (C), and RHBDF2 (D) in paired ccRCC tissues (n = 15). GAPDH was used as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001.