RPA2 [p Ser4, p Ser8] Antibody

Product Specs
ItemRPA2 [p Ser4, p Ser8] Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $549.00Supplier Page View Company Product Page
Catalog NumberNBP1-23017
Concentration1.0 mg/ml
ImmunogenImmunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID
ReactivityHuman,Mouse
HostRabbit
Quantity100 ul
FormatTris-Citrate/Phosphate (pH 7.0 - 8.0)
TargetRPA2
Conjugate/TagUnconjugated
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Knockdown Validated,Immunocytochemistry/ Immunofluorescence
Modificationsp Ser4, p Ser8
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaPhospho Specific
IsotypeIgG
Antibody TypePrimary Antibodies
StorageStore at 4C. Do not freeze
NCBI Full Gene Namereplication protein A2
NCBI Gene AliasesREPA2, RP-A p34, RPA32, RP-A p32

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 100 ul

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) p53-dependent crosstalk between DNA replication integrity and redox metabolism mediated through a NRF2-PARP1 axisNucleic acids researchNovember 11, 2024Elfar GA, Aning O, Ngai TW, Yeo P, Chan JWK, Sim SH, Goh L, Yuan J, Phua CZJ, Yeo JZZ, et al.

(2) HMGN1 loss sensitizes lung cancer cells to chemotherapyScientific reportsMay 6, 2024Wu X, Cai G, Feng J, Lin W.12302), CHK1 (1:1000, CST 2G1D5), β-actin (1:1000, TransGen HC201-02), p-RPA2 (1:1000,NOVUS,Ser4, Ser8, NBP1-23017), RPA2 (1:1000, Abcam ab2175), HMGN1 (1:1000, proteintech 11695-1-AP). Secondary antibodies were Rabbit

(3) Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA double-strand breaksThe Journal of biological chemistryMarch 1, 2024Chen L, Gai X, Yu X.4499S), anti-RAD51 (Abcam; rabbit, catalog no.: ab133534), and anti-RPA (Novus; rabbit, catalog no.: NBP1-23017). RNA interference Cells were transfected with siRNAs against nonspecific control or RAD51AP1(5 nM)

(4) Synergistic lethality between auranofin-induced oxidative DNA damage and ATR inhibition in cancer cellsLife sciencesNovember 1, 2023Zhang S, Zhao Y, Wang X, Qi C, Tian J, Zou Z.

(5) Pre-rRNA Facilitates TopBP1-Mediated DNA Double-Strand Break ResponseAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2023Xin D, Gai X, Ma Y, Li Z, Li Q, Yu X.(GeneTex, Mouse, GTX70107), anti‐pATM (S1981) (ROCKLAND, Mouse, 200‐301‐400), anti‐pRPA (Novus, Rabbit, NBP1‐23017), anti‐Rad9 (Santa Cruz, Mouse, sc‐74464), anti‐RAD51 (abcam, Rabbit, ab63801). PAR‐CLIP, RNA Sequencing,

(6) A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance geneNature communicationsJanuary 24, 2023Gao Y, Guitton-Sert L, Dessapt J, Coulombe Y, Rodrigue A, Milano L, Blondeau A, Larsen NB, Duxin JP, Hussein S, et al.

(7) RAD51 Is Implicated in DNA Damage, Chemoresistance and Immune Dysregulation in Solid TumorsCancersNovember 20, 2022Liao C, Talluri S, Zhao J, Mu S, Kumar S, Shi J, Buon L, Munshi NC, Shammas MA.Cell Signaling, Danvers, MA, USA), a marker of DNA breaks and RPA32 phosphorylated on ser4 and ser8 (pRPA32; CAS NBP1-23017; Novus Biologicals, Littleton, CO, USA), a marker of DNA end resection [29,30] by Western blotting.

(8) A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome InstabilitySSRN Electronic JournalApril 4, 2022Katerina Fagan-Solis et al.RRID:AB_244321 Rabbit Anti-RPA2, (phospho Ser4, Ser8) Polyclonal, Unconjugated antibody (1:500 for IF) Novus Cat# NBP1-23017, RRID:AB_1726226 Rabbit Anti-53BP1 Polyclonal Antibody (1:500 for IF) Bethyl Cat# A300-272A, RRID:AB_185520 Goat

(9) Induction of APOBEC3 Exacerbates DNA Replication Stress and Chromosomal Instability in Early Breast and Lung Cancer EvolutionCancer discoveryOctober 1, 2021Venkatesan S, Angelova M, Puttick C, Zhai H, Caswell DR, Lu WT, Dietzen M, Galanos P, Evangelou K, Bellelli R, et al.anti-RPA2(S33) antibody was not reactive against murine tissue, therefore the rabbit anti-RPA2 S4/S8 (NBP123017, Novus Biologicals) was used instead. For senescence detection by SenTraGor™, a GL13-based (a biotinylated

(10) MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repairNature communicationsSeptember 14, 2021de Krijger I, Föhr B, Pérez SH, Vincendeau E, Serrat J, Thouin AM, Susvirkar V, Lescale C, Paniagua I, Hoekman L, et al.Invitrogen, 1:500), H3 (ab1791, Abcam, 1:1000), H2B (07-371, Millipore, 1:1000), p-RPA (RPA2 pS4/pS8: NBP1-23017, Novus Biologicals, 1:500 and A300-245A, Bethyl, 1:1000), p-Kap1 (S824, A300-767A, Bethyl, 1:1000),

(11) Cinobufagin-induced DNA damage response activates G(2)/M checkpoint and apoptosis to cause selective cytotoxicity in cancer cellsCancer cell internationalAugust 23, 2021Niu J, Wang J, Zhang Q, Zou Z, Ding Y.(A300-272A) (Bethyl, Montgomery, TX, USA); Phospho-CDK1 (AF5761) (Beyotime, Shanghai, China); RPA2 (NBP1-23017) (NOVUS, Shanghai, China). Secondary antibodies were goat anti-mouse-Alexa 488 (115-545-003) and goat

(12) The dystonia gene THAP1 controls DNA double-strand break repair choiceMolecular cellJune 17, 2021Shinoda K, Zong D, Callen E, Wu W, Dumitrache LC, Belinky F, Chari R, Wong N, Ishikawa M, Stanlie A, et al.NB100-305; RRID: AB_10001695 "Rabbit polyclonal anti-RPA32/RPA2 (pS4, S8)",Novus Biologicals,Cat# NBP1-23017; RRID: AB_1726226 Rat monoclonal anti-RPA32/RPA2 (4E4),Cell Signaling Technology,Cat# 2208; RRID: AB_2238543 Mouse

(13) Integrated genomics and comprehensive validation reveal drivers of genomic evolution in esophageal adenocarcinomaCommunications biologyMay 24, 2021Kumar S, Buon L, Talluri S, Roncador M, Liao C, Zhao J, Shi J, Chakraborty C, Gonzalez G, Tai YT, et al.

(14) FK228 sensitizes radioresistant small cell lung cancer cells to radiationClinical epigeneticsFebruary 25, 2021Li H, Ma L, Bian X, Lv Y, Lin W.

(15) Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cellsOncogeneApril 1, 2020Wang H, Zhang S, Song L, Qu M, Zou Z.H3 (abs131869), H2AX (abs131731), CHK2 (abs131635) (Absin Bioscience, Shanghai, China); RPA32-pS4/8 (NBP1-23017) (NOVUS, Centennial, CO, USA), 53BP1 (A300-272A) (Bethyl, Montgomery, TX, USA), γH2AX-pS139 monoclonal

(16) Cancer cell stemness, responses to experimental genotoxic treatments, cytomegalovirus protein expression and DNA replication stress in pediatric medulloblastomasCell cycle (Georgetown, Tex.)April 1, 2020Bartek J Jr, Merchut-Maya JM, Maya-Mendoza A, Fornara O, Rahbar A, Bröchner CB, Sehested A, Söderberg-Nauclér C, Bartek J, Bartkova J.

(17) A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome InstabilityCell reportsFebruary 4, 2020Fagan-Solis KD, Simpson DA, Kumar RJ, Martelotto LG, Mose LE, Rashid NU, Ho AY, Powell SN, Wen YH, Parker JS, et al.RRID:AB_244321" "Rabbit Anti-RPA2, (phospho Ser4, Ser8) Polyclonal, Unconjugated antibody (1:500 for IF)",Novus,"Cat# NBP1-23017, RRID:AB_1726226" Rabbit Anti-53BP1 Polyclonal Antibody (1:500 for IF),Bethyl,"Cat# A300-272A, RRID:AB_185520" "Goat

(18) The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung CancerFrontiers in oncologyJanuary 1, 2020Bian X, Wang X, Zhang Q, Ma L, Cao G, Xu A, Han J, Huang J, Lin W.

(19) Differential Sensitivity to CDK2 Inhibition Discriminates the Molecular Mechanisms of CHK1 Inhibitors as Monotherapy or in Combination with the Topoisomerase I Inhibitor SN38ACS pharmacology & translational scienceJune 14, 2019Warren NJH, Donahue KL, Eastman A.

(20) Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastropheThe Journal of biological chemistryFebruary 8, 2019Warren NJH, Eastman A.

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