Fig 1: Confirmation of regulation of selected genes at 24 suture IN and OUT, by qPCR and immunohistochemistry.Gene expression confirmation by qPCR, and protein level expression by immunohistochemistry. Panel (a) indicates gene expression by qPCR, (b) indicates the microarray gene expression and (c) is the protein expression in the tissue for Vegfa, Cxcl5, Fgf7 and Rasa2, respectively. For qPCR, ANOVA p-values were 0.006, <0.001, <0.001 and 0.009, for Vegfa, Cxcl5, Fgf7 and Rasa2, respectively. Pairwise significance between groups with p < 0.05 is indicated by asterisks. For microarray, p-values between suture IN and suture OUT were 4.49E-04, 3.89E-04, 3.66E-04 and 8.56E-03, for Vegfa, Cxcl5, Fgf7 and Rasa2, respectively. The asterisks represent a significant difference p < 0.05 between groups. Error bars for qPCR and microarray represent standard deviation. Microarray data represents 4 corneas/group and qPCR data 3 corneas/group with 2 technical replicates/sample.