TGF alpha antibody orb248563 from Biorbyt

Description

TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody

Product Specs
ItemTGF alpha antibody
CompanyBiorbyt
Price $370.00Supplier Page
Catalog Numberorb248563
Quantity20 ug, 100 ug
ApplicationsFACS, IF, IHC-P
Conjugate/TagUnconjugated
ReactivityHuman
TargetTGF alpha
IsotypeMouse IgM, kappa
Clone1E8-G6
HostMouse
ImmunogenA 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

Specifications/Features

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Application Dilutions: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Add to Compare List