Expression of NCX2 in rat cerebellum. Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody, (1:100). A. NCX2 staining (red) is expressed in the molecular layer (M) and Purkinje cells (arrows). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of NCX2 expression to the molecular layer and Purkinje cells.