Expression of VPAC1 in rat amygdala. Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (extracellular) antibody, (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images.

Expression of VPAC1 in human HT-29 cells. Immunocytochemical staining of live intact human HT-29 colorectal adenocarcinoma cells. A. Extracellular staining of live cells with Anti-VPAC1 (extracellular) antibody, (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells.

Indirect flow cytometry analysis of live intact Jurkat (human T cell leukemia cells) cell line: black line Cells + goat-anti-rabbit-DyLight-488. Green line Cells + Anti-VPAC1 (extracellular) antibody, (1:20) + goat-anti-rabbit-DyLight-488.