Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RAPGEF1 mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RAPGEF1mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-RAPGEF clone UMAB140 mouse monoclonal antibody. HIER ACCEL buffer (B22C-125) (pH8.7) at 110C for 10 min, UM500102 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).