Fig 1: Neurotensin/neuromedin N expression in the heart of rats with pulmonary arterial hypertension (PAH). Male Sprague Dawley rats were subcutaneously injected with SU5416 (20 mg/kg body weight), maintained in hypoxia in a chamber set to maintain 10% O2 [12,26] for three weeks and then in normoxia for 17 weeks [27,28]. Heart tissues were immersed in buffered 10% formalin, embedded in paraffin, cut and mounted on glass slides. Tissue sections were subjected to immunohistochemistry using the neurotensin/neuromedin N (NTS) antibody (catalog # MBS8505326; MyBioSource, Inc., San Diego, CA, USA). (A) Representative immunohistochemistry images of the RVs from 3 pairs of control and PAH animals. (B) The bar graph represents means ± SEM of % NTS antibody-negative cells in the LV and RV. * denotes values significantly different from each other at p < 0.001.
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