The Calnexin-CT Antibody: RPE from MyBioSource.com is a Rabbit Polyclonal antibody to Calnexin, CANX, and IP90. This antibody recognizes Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Drosophila/Arthropod, Guinea Pig, Hamster, Non-Human Primate, Porcine, Rabbit, Sheep, and Xenopus/Amphibian antigen. The Calnexin-CT Antibody: RPE has been shown to work in the following applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.
Description
Background Info: Detects ~90kDa.
Scientific Background: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7)