Mouse anti Lactoferrin MBS570346 from MyBioSource.com

Description

Lactoferrin (LF) is an iron-binding protein with bactericidal and bacteriostatic activity, which is stored within the secondary granules of granulocytes. LF expression is restricted to the post-mitotic maturation compartment of the granulocytic lineage, starting from the myelocyte stage. Normal and malignant myeloblasts are LF negative.

The 4C5 antibody permits the identification and enumeration of human granulocytes using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analysis performed with this antibody should be paralleled by positive and negative controls

Product Specs
ItemMouse anti Lactoferrin
CompanyMyBioSource.com
Price $280.00Supplier Page View Company Product Page
Catalog NumberMBS570346
Quantity0.2 mg
ApplicationsFlow Cytometry (FC/FACS), Indirect Immunofluorescence (IF)
Conjugate/TagUnconjugated
FormatPurified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3
ReactivityHuman
TargetLactoferrin
IsotypeIgG1
Molecular Weight73,161 Da
Clone4C5
HostMouse
ImmunogenThe LF mAb (clone 4C5) recognizes Lactoferrin stored within secondary granules of postmitotic granulocyte-committed cells.

The sensitivity of 4C5 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7
cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity
NCBI Full Gene Namelactotransferrin
NCBI Gene AliasesGIG12, HEL110, HLF2, LF
ClonalityMonoclonal