FLAG antibody MBS533391 from MyBioSource.com

Description

Mouse monoclonal FLAG antibody
FLAG tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis

Product Specs
ItemFLAG antibody
CompanyMyBioSource.com
Price $405.00Supplier Page View Company Product Page
Catalog NumberMBS533391
Quantity0.1 mg
ApplicationsDot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Concentration1 mg/ml
Conjugate/TagUnconjugated
FormatSupplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3
PurityFLAG antibody was purified by Protein A affinity chromatography
TargetFLAG
IsotypeIgG2b
CloneFG4R
HostMouse
ImmunogenFLAG tag
ClonalityMonoclonal

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Questions and Answers

Can this FLAG antibody be used for studying protein interactions in complex mixtures?

Yes, the FLAG antibody is suitable for isolating protein complexes with multiple subunits. By tagging proteins with the FLAG sequence, researchers can utilize this antibody to pull down and study interactions within complex mixtures.

What applications can I use this FLAG antibody for in my experiments?

The FLAG antibody can be employed in various applications including Western Blotting, ELISA, Immunoprecipitation, and Detection by SDS PAGE protein electrophoresis. These applications allow for effective detection and analysis of FLAG-tagged proteins.

Is this antibody effective for cellular localization studies?

Yes, the FLAG antibody can be used for cellular localization studies through immunofluorescence. By tagging proteins with the FLAG sequence, researchers can visualize the localization of these proteins within cells using this antibody.

What is the host species for this FLAG antibody, and what is its isotype?

The FLAG antibody is a mouse monoclonal antibody, specifically of the IgG2b isotype. This information is important for understanding its compatibility with various experimental setups and potential cross-reactivity.