Fig 1: Low‐dose ATO downregulated expression of stemness‐related genes and inhibited self‐renewal and tumorigenic capacity of HCC CSCs. (A) Relative mRNA expression of stemness‐related genes OCT4, NOTCH1, SOX2, ABCG2, and NANOG altered by ATO treatment in Huh7, Hep3B, and primary HCC cells. (B) Immunofluorescent staining of stemness‐related proteins ABCG2, SOX2, OCT4, and CD133 in Huh7 cell spheres with or without ATO treatment (magnification: 200×). (C) ATO impaired sphere‐forming ability of Huh7 and Hep3B cells (magnification: 200×). (D) Upon exposure to ATO, the number of clones diminished dramatically in Huh7 cells and Hep3B cells. (E) NOD/SCID mice were subcutaneously inoculated with CD133+ or CD133‐ Huh7 cells to explore the tumorigenic potential altered by ATO