Fig 1: NK92-Neo2/15-OX40L promotes an OX40-OX40L positive feedback loop (*p < 0.05). (A) Western-blot detection of NK-κB activation for indicated treatment. (B) 4-1BB and OX40 expression in expanded NK cells from 5 donors, measured by Western-blot before and after feeder cell treatment. (C) Relative gray value statistics of protein level in (B). (D) Immunoblot of TRAF5, phosphorylated-p65, and p65 was detected. (E) FACS analysis of OX40L expression on expanded NK. (F) NK cells from PBMC, treated by IL-2 or NK92-Neo2/15-OX40L expanded NK cells at a ratio of 19:1, freshly collected NK cells were used as control. Phosphorylated p65 were determined by western blot.