Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) ab199376 from Abcam

Product Specs
ItemRabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab199376
Antibody TypeIsotype Control Antibody
ClonalityMonoclonal
ApplicationsWB, FCM, IA, IHC-p, IP, ChIP-seq, ICC, IF
HostRabbit
CloneEPR25A
IsotypeIgG
Conjugate/TagUnconjugated
Quantity1 mg, 100 µg

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Maternal causation of early-onset pre-eclampsia: excessive endometrial gland-derived apolipoprotein D induces placental ferroptosis and developmental abnormalitiesJ Biomed SciDecember 10, 2025Yang Dong, Cheuk-Lun Lee, Jianlin Li, Xiaofeng Liu, Qunxiong Zeng, Jiangming Zhong, Qingqing Zhang, Ting Wu, Vivian W. Y. Ng, Calvin K. F. Lee, et al.ab108422 Abcam, Cambridge), anti-PAX8 antibody (1:100, ab227707,Abcam, Cambridge) and Isotype (1:100, ab199376, Abcam, Cambridge). To examine the localization and expression of APOD, the sections were stained overnight

(2) Efficacy of modified-vaccinia Ankara vaccine as pre- and post-exposure prophylaxis against monkeypox sexual transmission in non-human primate modelNat CommunAugust 7, 2025Cécile Herate, Audrey Ferrier-Rembert, Francis Relouzat, Anne-Sophie Gallouët, Quentin Pascal, Hélène Letscher, Mariangela Cavarelli, Nathalie Dereuddre-Bosquet, Wesley Gros, Benoit Delache, et al.µg/ml Calprotectine,MAC387,MA5-12213,ThermoScientific,WA3163015,0.1 µg/ml Vaccinia,Polyclonal,Ab35219,Abcam,1022881-2,1 µg/ml IgG Isotype,EPR25A,Ab199376,Abcam,GR3179509-8,1 µg/ml IgG1 Isotype,k Isotype,556648,BD,8181938,1 µg/ml IgG2a Isotype,k isotype,556651,BD,9352875,0.1

(3) Exosome-Delivered EGFR Induced by Acidic Bile Salts Regulates Macrophage M2 Polarization to Promote Esophageal Adenocarcinoma Cell ProliferationOncoTargets and therapyJanuary 1, 2024Chen C, Ding J, Ma Z, Xie Y, Zhang L, Zhu D.polyclonal,Immunohistochemistry,Sigma Anti-CCL18,ab9849,Rabbit polyclonal,Neutralising,Abcam IgG isotype control,ab199376,Rabbit,Neutralising,Abcam Discription of Antibodies Used in the Study

(4) Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulationCell Death DiscoveryDecember 1, 2022Giuseppina E. Grieco et al.anti-cleaved Caspase-3 (cat. ab13847, Abcam) antibody [or 10 μg/mL of rabbit IgG Isotype Control (cat. ab199376 Abcam)] and 1:500 diluted secondary antibody Goat anti-rabbit Alexa Fluor 594 (cat. A11037, Thermo Fisher Scientific

(5) Critical role of FPR1 in splenocyte migration into brain to worsen inflammation and ischemic brain injury in miceTheranosticsJanuary 1, 2022Li J, Chordia MD, Zhang Y, Zong H, Pan D, Zuo Z.immune-labeling was confirmed by incubating sections with pre-immune rabbit IgG (1:250; catalog number: ab199376; Abcam) in place of primary antibody. Under these conditions, no detectable labeling was observed. Images

(6) CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1Scientific reportsJuly 21, 2021Durlanik S, Fundel-Clemens K, Viollet C, Huber HJ, Lenter M, Kitt K, Pflanz S.received either 20 µg/ml αCD276 antibody (ab209895, Abcam) or 20 µg/ml monoclonal rabbit IgG isotype (ab199376, Abcam) for 4 days. Flow cytometry Single cell suspension was washed with 0.2% BSA-PBS and directly

(7) Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor β and S100A8/9 up-regulationJournal of cellular and molecular medicineDecember 1, 2020Wang Z, Zheng G, Li G, Wang M, Ma Z, Li H, Wang XY, Yi H.(ab150113; Abcam). Isotype controls used Rabbit IgG, polyclonal (ab171870; Abcam), Rabbit IgG, monoclonal (ab199376; Abcam), and Mouse monoclonal IgG1(ab170190; Abcam) from Abcam. For intracellular staining, cells were

(8) Glycolysis-Independent Glucose Metabolism Distinguishes TE from ICM Fate during Mammalian EmbryogenesisDevelopmental cellApril 6, 2020Chi F, Sharpley MS, Nagaraj R, Roy SS, Banerjee U.isotype control (MG1–45),Abcam,Cat #ab18447 Rabbit IgG monoclonal isotype control (EPR25A),Abcam,Cat #ab199376 Mouse monoclonal anti-CDX2 (CDX2–88),Biogenex,Cat #MU392A-UC Goat anti-OCT4,Santa Cruz,Cat #sc-8628 Rabbit

(9) SARS-CoV-2 Receptor Angiotensin I-Converting Enzyme Type 2 (ACE2) Is Expressed in Human Pancreatic β-Cells and in the Human Pancreas MicrovasculatureFrontiers in endocrinologyJanuary 1, 2020Fignani D, Licata G, Brusco N, Nigi L, Grieco GE, Marselli L, Overbergh L, Gysemans C, Colli ML, Marchetti P, et al.(cat. X0943-Agilent Technologies, Santa Clara, CA, USA) or negative isotype control rabbit IgG (cat. Ab199376, Abcam, Cambridge, UK) for 60 min. Next, EndoC-βH1 cells were rinsed with PBS without Ca2+ and Mg2+

(10) Regulation of Co-transcriptional Pre-mRNA Splicing by m⁶A through the Low-Complexity Protein hnRNPGMolecular cellOctober 3, 2019Zhou KI, Shi H, Lyu R, Wylder AC, Matuszek Ż, Pan JN, He C, Parisien M, Pan T.Cell Signaling Technology; Ab #3: NBP2–34152, Novus Biologicals), or 20 μg/mL rabbit isotype control antibody (ab199376, Abcam) in a total volume of 90–100 μL per 100-mm plate of cells. After rotating at 4 °C overnight,

(11) Decreased Level of Neurotrophic Factor Neuritin 1 in Women with Ovarian Endometriosis after Receiving Gonadotropin-Releasing Hormone Agonist TreatmentInternational Journal of Molecular SciencesSeptember 5, 2019Endah Rahmawati, Wei-Chung Vivian Yang, Yen-Ping Lei, Pawan Kumar Maurya, Huei-Wen Chen, Chii-Ruey Tzengtreated identically with the exception that the primary antibody was replaced with a rabbit IgG isotype control (ab199376; Abcam). Images were captured using the SPOT imaging software. 4.5. Enzyme-Linked Immunosorbent Assay The

(12) Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophageNature immunologyFebruary 1, 2019Aran D, Looney AP, Liu L, Wu E, Fong V, Hsu A, Chak S, Naikawadi RP, Wolters PJ, Abate AR, et al.presence of Pdgf-aa blocking antibody (catalog number 07–1436, Millipore) or IgG control (catalog number ab199376, lot GR3195151–2, Abcam). EDU was added at 48 hours, and cells were suspended two hours later for EDU

(13) Age-Related Upregulation of Carboxyl Terminal Modulator Protein Contributes to the Decreased Brain Ischemic Tolerance in Older RatsMolecular neurobiologyJuly 1, 2018Li J, Shan W, Zuo Z.immune-labeling was confirmed by incubation of sections with pre-immune rabbit IgG (1:250, catalog number: ab199376; Abcam) in place of primary antibody. No detectable labeling was observed under these conditions. Images

(14) SNHG6 Promotes Tumor Growth via Repression of P21 in Colorectal CancerCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Li Z, Qiu R, Qiu X, Tian T.manufacturer’s instructions. Antibodies for RIP-assay were EZH2 (21800-1-AP, Proteintech) or normal IgG, (ab199376, Abcam). Chromatin Immunoprecipitation (ChIP) Assay The ChIP assays were performed as previously reported

(15) HMGB1 suppresses colon carcinoma cell apoptosis triggered by co‑culture with dendritic cells via an ER stress‑associated autophagy pathwayMolecular Medicine ReportsDecember 6, 2017Yingcheng Zheng, Guoguo Zhuv/v) was applied to neutralize HMGB1 in cell cultures, and rabbit IgG monoclonal antibody (cat. no. ab199376; Abcam; 1:50) was used as its isotype control. RNA transfection. Small interfering (si)RNA duplexes

(16) Efficient Differentiation of TBX18⁺/WT1⁺ Epicardial-Like Cells from Human Pluripotent Stem Cells Using Small Molecular CompoundsStem cells and developmentApril 1, 2017Zhao J, Cao H, Tian L, Huo W, Zhai K, Wang P, Ji G, Ma Y.Biotech, Tianjin, China) were used as secondary antibodies. Goat IgG (sc3887; Santa Cruz), Rabbit IgG (ab199376; Abcam), mouse IgG1-PE (IC002P; R&D), and mouse IgG1 (M5284; Sigma) were used as isotype controls. Samples

(17) Suramin increases cartilage proteoglycan accumulation in vitro and protects against joint damage triggered by papain injection in mouse knees in vivoRMD openJanuary 1, 2017Guns LA, Monteagudo S, Kvasnytsia M, Kerckhofs G, Vandooren J, Opdenakker G, Lories RJ, Cailotto F.were treated at day 1 with 10 µg of anti-TIMP3 (Abcam ab39184) or 10 µg of isotype control IgG (Abcam ab199376). Antibody treatments were renewed every 3 days. Plates were collected at days 7 and 14. Each time point

(18) Retargeted adenoviruses for radiation-guided gene deliveryCancer gene therapySeptember 1, 2016Kaliberov SA, Kaliberova LN, Yan H, Kapoor V, Hallahan DE.105 cells were incubated with anti-GRP78 rabbit monoclonal antibody or isotype control (ab108614 and ab199376, respectively, Abcam, Cambridge, MA, USA) for 1 h on ice. After washing with PBS, cells were incubated

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Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) from Abcam

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Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) from Abcam

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