Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093 from Abcam

Product Specs
ItemAlexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab199093
HostRabbit
Antibody TypeIsotype Control Antibody
ReactivityHuman (Hu)
ClonalityMonoclonal
ApplicationsFCM, IHC-p, ICC, IF
CloneEPR25A
IsotypeIgG
Conjugate/TagAlexa Fluor® 647
Concentration0.5 mg/ml
Quantity100 µL

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Characterisation of Bespoke Patient-Derived In Vitro Models of Ewing SarcomaCancers (Basel)February 4, 2026Elizabeth A. Roundhill, Elton J. R. Vasconcelos, John Davies, Susan A. Burchill(Thermo Fisher Scientific), Negative Control Mouse IgG1, X0931 (Dako), Alexa Fluor 647 and 488 Rabbit IgG isotype controls (ab199093 and ab199091, Abcam Plc.)). Expression was detected for unconjugated primary antibodies using a goat

(2) Targeting tumor metabolic flexibility enhances radiotherapeutic efficacy via mitochondrial complex I Inhibition in an intracranial S180 sarcoma mouse modelScientific ReportsNovember 28, 2025Zhipeng Liu, Sa-Hoe Lim, Shin Junganti-p53 antibody (Abcam, USA; ab302697) at a 1:500 dilution. An isotype control antibody (Abcam, USA; ab199093) was used at the same concentration and under identical conditions as the primary antibody. Flow cytometry

(3) Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human braineLifeSeptember 25, 2025Bonnifet T, Sinnassamy S, Massiani-Beaudoin O, Mailly P, Monnet H, Loew D, Lombard B, Servant N, Joshi RL, Fuchs J.the coupled antibody NeuN Alexa 647 (Abcam, ab190565) or the control isotype IgG Alexa 647 (Abcam, ab199093). Stained cells were filtered a last time with a 40-µm filter before FACS sorting (FACS ARIA II). Neuronal

(4) Hepatic ASPG-mediated lysophosphatidylinositol catabolism impairs insulin signal transductionThe EMBO JournalAugust 4, 2025Feiyan Li, Hua-Sheng Huang, Qingwen Zhao, Wei Zhang, Ting Shi, Wenjing Lv, Qi Zhu, Haojie Liu, Yingjiang Xu, Haiyan Huang, et al.obtain 400–500 bp DNA fragments. The sonicated DNA solution was incubated with 2 μg control IgG (Abcam, #ab199093) or anti-FOXO1 antibody (Cell Signaling Technology, #2880) overnight at 4 °C with rotation. Protein

(5) In vitro HIV DNA integration in STAT3 drives T cell persistence-A model of HIV-associated T cell lymphomaPLoS PathogJuly 8, 2025Michael Rist, Machika Kaku, John M. CoffinCat#: ab300101) and GZMB (Biolegend, Cat#: 372219). Isotype control for STAT3 staining (Abcam, Cat#: ab199093) and isotype control for GZMB (Biolegend, Cat#: 400130). For pSTAT3 staining, T cells were fixed and

(6) miR-23a-mediated TRF2 repression in CD4 T cells from PLWHMolecular ImmunologyJune 1, 2025Lam N.T. Nguyen, Juan Zhao, Jaeden S. Pyburn, Ling Wang, Madison Schank, Puja Banik, Addison C. Hill, Xiao Y. Wu, Yi Zhang, Shunbin Ning, et al.350514) (all from Biolegend); Alexa Fluor 647 Anti-TRF2 (Cat# ab198597), Alexa Fluor 647 Rabbit IgG (Cat# ab199093) (all from Abcam, Cambridge, MA). For apoptosis analysis, the PE and FITC Annexin V apoptosis detection

(7) Role of N-linked glycosylation sites in human ACE2 in SARS-CoV-2 and hCoV-NL63 infectionJournal of virologyMay 20, 2025Noettger S, Zech F, Nchioua R, Pastorio C, Jung C, Jacob T, Stenger S, Kirchhoff F.(eBioscience, #65-0863-14), anti-ACE2 AF647 (Abcam, #ab283658), and anti-IgG Isotype control AF647 (Abcam, #ab199093) antibodies, and flow cytometric measurements were performed using a BD FACS Canto II flow cytometer.

(8) α-Synuclein fibrils enhance HIV-1 infection of human T cells, macrophages and microgliaNat CommunJanuary 18, 2025Lia-Raluca Olari, Sichen Liu, Franziska Arnold, Julia Kühlwein, Marta Gil Miró, Ajeet Rijal Updahaya, Christina Stürzel, Dietmar Rudolf Thal, Paul Walther, Konstantin M. J. Sparrer, et al.Cat: 304005 and isotype Cat: 400107), Alexa Fluor® 647 anti-human IBA1 Ab (Abcam, Ab225261 and isotype Ab199093), Brilliant Violet 605™ anti-human CD4 Ab (BioLegend Cat: 317438 and isotype Cat: 400350), APC anti-human

(9) DNA-PKcs inhibition improves sequential gene insertion of the full-length CFTR cDNA in airway stem cellsMolecular Therapy - Nucleic AcidsDecember 10, 2024Jacob T. Stack, Rachael E. Rayner, Reza Nouri, Carlos J. Suarez, Sun Hee Kim, Karen L. Kanke, Tatyana A. Vetter, Estelle Cormet-Boyaka, Sriram VaidyanathanAF647-anti-KRT5 (Abcam; ab193895), and anti-P63 (Biolegend; 687202). Isotype antibodies AF647 Rabbit IgG (Abcam; ab199093) for KRT5 and Mouse IgG2b (Biolegend; 401202) for P63 were used. The P63 antibody and isotype were unconjugated

(10) Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertionCell Rep MethodsMay 22, 2023Tompkins J, Lizhar E, Shokrani A, Wu X, Berley J, Kamali D, Hussey D, Cerneckis J, Kang TH, Wang J, et al.conducted according to prior CM methylation mapping work.21 Antibodies included: K5 isotype (Abcam, ab199093), human K5 (Abcam, ab193895), K8 isotype (Abcam, ab91356), or human K8 (Abcam, ab176533). Samples were

(11) Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane DamageAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2023Raj N, Greune L, Kahms M, Mildner K, Franzkoch R, Psathaki OE, Zobel T, Zeuschner D, Klingauf J, Gerke V.(EPR12790) (1:100, Abcam, ab198949) and the corresponding isotype control rabbit α‐IgG (1:100, Abcam, ab199093) were used. Immunofluorescence stainings employed the following secondary antibodies: goat α‐rabbit

(12) RAGE inhibition alleviates lipopolysaccharides-induced lung injury via directly suppressing autophagic apoptosis of type II alveolar epithelial cellsRespiratory researchJanuary 24, 2023Xiong X, Dou J, Shi J, Ren Y, Wang C, Zhang Y, Cui Y.Cell Signaling Technology, MA, USA), anti-RAGE (1:1000, ab37647; Abcam, Cambridge, Mass.), and rabbit IgG isotype (1:1000, ab199093; Abcam, Cambridge, Mass.) at 4 °C overnight, followed by incubation with fluorescently labelled secondary

(13) BTG1 mutation yields supercompetitive B cells primed for malignant transformationScience (New York, N.Y.)January 20, 2023Mlynarczyk C, Teater M, Pae J, Chin CR, Wang L, Arulraj T, Barisic D, Papin A, Hoehn KB, Kots E, et al.following isotype control IgGs at the same concentration as their corresponding anti-MYC antibodies: Abcam ab199093, clone EPR25A, for the AF647 anti-MYC antibody; and Abcam ab172730, clone EPR25A, for the unconjugated

(14) A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutationsNature communicationsNovember 14, 2022Deng H, Lei Q, Wang C, Wang Z, Chen H, Wang G, Yang N, Huang D, Yu Q, Yao M, et al.Alexa Fluor 647 anti-EGFR (#ab192982, Abcam, 1:100), and isotype control (Alexa fluor 647 Rabbit IgG, ab199093, Abcam, 1:100). The data analysis of multicolor FACS experiments and the calculated ratios of EGFR(+)HX103(+)

(15) Oncolytic Herpes Simplex Virus Type 1 Induces Immunogenic Cell Death Resulting in Maturation of BDCA-1(+) Myeloid Dendritic CellsInternational journal of molecular sciencesApril 27, 2022Kalus P, De Munck J, Vanbellingen S, Carreer L, Laeremans T, Broos K, Dufait I, Schwarze JK, Van Riet I, Neyns B, et al.anti-calreticulin-AF700 (1/100; Abcam, Cambridge, UK; ab196195) antibody or rabbit Ig G isotype control (1/100; Abcam, ab199093) and 49.5 µL FACS buffer. Cells were incubated for 30 min at 4 °C and in the dark. Afterwards, cells

(16) MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinomaCancer communications (London, England)December 1, 2021Wu Q, Zhang W, Wang Y, Min Q, Zhang H, Dong D, Zhan Q.Fluor 647 (1:100; ab209960; Abcam, Cambridge, UK,) and the control were incubated with isotype (1:100; ab199093; Abcam) at 4°C for 30 min. After incubation, the cells were washed twice and suspended in phosphate‐buffered

(17) Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarkerNature cell biologyJune 1, 2021Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.and 0.25 μg of its recommended Alexa Fluor 647-conjugated Isotype Control rabbit IgG [EPR25A] (Abcam #ab199093); 0.5 μg of APC-conjugated ITGB1/CD29 [TS2/16] (BioLegend #303007) and 0.5 μg of its recommended isotype

(18) Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune SurveillancePancreasMarch 1, 2021Fujiwara Y, Tsunedomi R, Yoshimura K, Matsukuma S, Fujiwara N, Nishiyama M, Kanekiyo S, Matsui H, Shindo Y, Tokumitsu Y, et al.NJ), and anti-CD47 PE (556046; BD). Rabbit immunoglobulin G (IgG) Isotype Control Alexa Fluor 647 (ab199093; Abcam), Mouse IgG1 Isotype Control FITC (A07795; BECKMAN COULTER, Tokyo, Japan), recombinant human

(19) Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomesOncology lettersJanuary 1, 2021Fujimoto T, Tsunedomi R, Matsukuma S, Yoshimura K, Oga A, Fujiwara N, Fujiwara Y, Matsui H, Shindo Y, Tokumitsu Y, et al.eBioscience; Thermo Fisher Scientific, Inc.), rabbit IgG Isotype Control Alexa Fluor 647 (cat. no. ab199093; Abcam) and mouse IgG2a κ monoclonal Isotype Control (cat. no. ab18415; Abcam) were used as nega‑ tive

(20) High-Efficiency, Selection-free Gene Repair in Airway Stem Cells from Cystic Fibrosis Patients Rescues CFTR Function in Differentiated EpitheliaCell stem cellFebruary 6, 2020Vaidyanathan S, Salahudeen AA, Sellers ZM, Bravo DT, Choi SS, Batish A, Le W, Baik R, de la O S, Kaushik MP, et al.RRID: AB_2728796 "Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647)",Abcam,Cat#: ab199093; RRID: AB_2818935 Alexa Fluor® 488 anti-human/mouse CD49f Antibody,Biolegend,Cat#: 313608 ;RRID: AB_493635 Anti-Cytokeratin

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Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control from Abcam

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Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control from Abcam

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