Fig 1: TRAM deletion abrogates the molecular machineries responsible for elastase productionWT and Tram−/− murine neutrophils were treated with oxLDL (10 μg/mL) for 48 h.(A) Secretion of elastase by neutrophils was quantified by ELISA (n = 5).(B) Neutrophils were labeled with CellROX, and ROS levels were quantified by FACS (n = 5).(C) Neutrophils were stained with anti-PMP70 and anti-LAMP1 antibodies, and the localization of peroxisomes and lysosomes was examined by confocal microcopy. Scale bar, 5 μm.(D) Confocal microscopy imaging of the subcellular distribution of PMP70+ peroxisomes and CaMKII in neutrophils. Scale bar, 5 μm.(E) Confocal microscopy imaging of the subcellular distribution of PMP70+ peroxisomes and SYK in neutrophils. Scale bar, 5 μm.(F) Protein level of SESN1 was examined by western blotting, and relative expression was normalized to β-actin (n = 3). Data are presented as means ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; one-way ANOVA.