Western blot analysis: Cell lysates of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 antibody (1/2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry: -N PDCD4 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry: -N PDCD4 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry: -N PDCD4 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.