Cas9 ( S. pyogenes ) (7A9-3A3) Mouse mAb 14697S from Cell Signaling Technology

Product Specs
ItemCas9 ( S. pyogenes ) (7A9-3A3) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number14697S
Quantity100 µl
ApplicationsW, IF-F, IF-IC, FC-FP
ReactivityAll
TargetCas9 bacteria
HostMouse
ClonalityMonoclonal
Research AreaRelated Products
Molecular Weight160

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) AMPK activation induces immunogenic cell death in AMLBlood AdvancesDecember 26, 2023Johanna Mondesir, Margherita Ghisi, Laura Poillet, Robert A. Bossong, Oliver Kepp, Guido Kroemer, Jean-Emmanuel Sarry, Jérôme Tamburini, Andrew A. Lane(Cell Signaling Technology, no. 2532S); CALR (Cell Signaling Technology, no. 12238S); Cas9 (Cell Signaling Technology, no. 14697S); eIF2α (Cell Signaling Technology, no. 9722S); phospho-AMPKα (Thr172); Cell Signaling Technology no.

(2) Efficient gene editing in induced pluripotent stem cells enabled by an inducible adenine base editor with tunable expressionSci RepDecember 11, 2023Krittika Nandy, Dinesh Babu, Sonam Rani, Gaurav Joshi, Smitha Ijee, Anila George, Dhavapriya Palani, Chitra Premkumar, Praveena Rajesh, S. Vijayanand, et al.Triton X-100 for 45 min and incubated with anti-SpCas9 antibody (Cell Signaling Technology Cat. No. 14697) at a 1:200 dilution in the blocking buffer overnight at 4 °C, followed by incubation with goat anti-mouse

(3) Non-Toxicological Role of Aryl Hydrocarbon Receptor in Obesity-Associated Multiple Myeloma Cell Growth and SurvivalCancers (Basel)November 1, 2023Jonathan D. Diedrich, Craig E. Cole, Matthew J. Pianko, Justin A. Colacino, Jamie J. Bernard(1:1000). The rabbit anti-human AhR (83200S), Histone H3 (4499S), GAPDH (2118S), and mouse-anti Cas9 (14697T) were purchased from Cell Signaling Technologies (Beverly, MA, USA). The rabbit-anti human Actin was

(4) Guide-specific loss of efficiency and off-target reduction with Cas9 variantsNucleic Acids ResearchOctober 13, 2023Liang Zhang, Wei He, Rongjie Fu, Shuyue Wang, Yiwen Chen, Han Xufollowed by blocking with 5% non-fat milk and incubation with primary antibody against Cas9 (CST, #14697, 1:2000), YAP1 (CST, #14074, 1:2000), MTAP (CST, #4158, 1:2000) or β-actin (Sigma, #A5316, 1:10000).

(5) Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse developmentNature structural & molecular biologyOctober 1, 2023Bona N, Crossan GP.(1:500, Proteintech 20285-1-AP), anti-XRCC3 (1:600, Proteintech 18494-1-AP); anti-Cas9 (1:1,000, 7A9-3A3 14697, Cell Signaling); anti-FLAG (1:200, M2 clone, F1804, Sigma-Aldrich); anti-LAMIN B1 (1:500, ab16048,

(6) Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunityNature CommunicationsSeptember 15, 2023Yazhong Cui, Yang Miao, Longzhi Cao, Lifang Guo, Yue Cui, Chuanzhe Yan, Zhi Zeng, Mo Xu, Ting HanDanvers, USA; 1:2000), anti-p-CREB (#9198; Cell Signaling Technology, Danvers, USA; 1:2000), anti-Cas9 (#14697; Cell Signaling Technology, Danvers, USA; 1:2000), anti-GNAS (#10150-2-AP; Proteintech, Rosemont, USA;

(7) Nanoblades allow high-level genome editing in murine and human organoidsMolecular Therapy - Nucleic AcidsSeptember 12, 2023Victor Tiroille, Adrien Krug, Emma Bokobza, Michel Kahi, Mattijs Bulcaen, Marjolein M. Ensinck, Maarten H. Geurts, Delilah Hendriks, François Vermeulen, Frédéric Larbret, et al.PBS/0.05%Tween/3%BSA (Sigma). Subsequently, the wells were washed and the primary anti-Cas9 antibody (Cas9-7A9-3A3, 14697P; Cell Signaling Technology) was added at 1/1,000 dilution in PBS/3%BSA, and incubated at room temperature

(8) Systematic characterization of therapeutic vulnerabilities in Multiple Myeloma with Amp1q reveals increased sensitivity to the combination of MCL1 and PI3K inhibitorsbioRxivAugust 3, 2023Romanos Sklavenitis-Pistofidis, Elizabeth D. Lightbody, Mairead Reidy, Junko Tsuji, Michelle P. Aranha, Daniel Heilpern-Mallory, Daisy Huynh, Stephen J. F. Chong, Liam Hackett, Nicholas J. Haradhvala, et al.LentiCas9-Blast (Addgene #52962) and Cas9 expression was confirmed by Western Blot (Cell Signaling Technology #14697). Subsequently, Cas9-positive cells were virally transduced with subpools of the Avana library of single

(9) Vascular endothelial profilin-1 drives a protumorigenic tumor microenvironment and tumor progression in renal cancerThe Journal of biological chemistryAugust 1, 2023Gau D, Daoud A, Allen A, Joy M, Sagan A, Lee S, Lucas PC, Duensing S, Boone D, Osmanbeyoglu HU, et al.Conditions for the various antibodies for immunoblotting purpose were as follows: monoclonal Cas9 (CST, 14697; 1:1000), monoclonal Tubulin (Sigma, T9026, 1:3000), polyclonal VHL (Thermo Fisher Scientific, PA5-87428;

(10) Deep Characterization and Comparison of Different Retrovirus-like Particles Preloaded with CRISPR/Cas9 RNPsInternational Journal of Molecular SciencesJuly 13, 2023Max Wichmann, Cecile L. Maire, Niklas Nuppenau, Moataz Habiballa, Almut Uhde, Katharina Kolbe, Tanja Schröder, Katrin Lamszus, Boris Fehse, Dawid Główand subsequently incubated with 100 μL diluted (s. below) anti-Cas9-antibody (mouse, Cas9-7A9-3A3 #14697; Cell Signaling Technology, Danvers, MA, USA) at 21 °C for 1h. Four washing steps with 300 μL washing

(11) Functional mapping of microRNA promoters with dCas9 fused to transcriptional regulatorsFrontiers in GeneticsMay 5, 2023Pradeep Kumar, Mathilde Courtes, Céline Lemmers, Anne Le Digarcher, Ilda Coku, Arnaud Monteil, Charles Hong, Annie Varrault, Runhua Liu, Lizhong Wang, et al.using antibodies directed against (species; provider; catalogue number): CAS9 (mouse; Cell signalling; #14697); NANOG (mouse; BD Pharmingen; #560259); NESTIN (mouse; Santa Cruz; sc-33677); PAX6 (mouse; Covance;

(12) Genome-wide CRISPR Screen Reveals RAB10 as a Synthetic Lethal Gene in Colorectal and Pancreatic Cancers Carrying SMAD4 LossCancer Research CommunicationsMay 4, 2023Hélène Erasimus, Vanessa Kolnik, Frédéric Lacroix, Sukhvinder Sidhu, Stéphane D'Agostino, Olivier Lemaitre, Alexandre Rohaut, Isabelle Sanchez, Gilbert Thill, Michel Didier, et al.anti-SMAD4 1:200 (#ab40759, Abcam), anti-GAPDH 1:200 (#2118, Cell Signaling Technology), anti-Cas9 1:200 (#14697, Cell Signaling Technology), anti-RAB10 1:100 (#8127, Cell Signaling Technology), anti SMAD3 1:100 (#9523),

(13) A kinome-wide CRISPR screen identifies CK1α as a target to overcome enzalutamide resistance of prostate cancerCell Reports MedicineApril 18, 2023Jinghui Liu, Yue Zhao, Daheng He, Katelyn M. Jones, Shan Tang, Derek B. Allison, Yanquan Zhang, Jing Chen, Qiongsi Zhang, Xinyi Wang, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Mouse monoclonal anti-Cas9,Cell Signaling Technology,Cat# 14697; RRID:AB_2750916 Rabbit monoclonal anti-AR,Cell Signaling Technology,5153Cat# 5153; RRID:AB_10691711 Rabbit

(14) Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic miceNature communicationsMarch 30, 2023Yan N, Feng H, Sun Y, Xin Y, Zhang H, Lu H, Zheng J, He C, Zuo Z, Yuan T, et al.least 1 h at room temperature, and reacted with primary antibodies including Cas9 (7A9-3A3) mouse mAb (14697S, Cell Signaling Technology, 1:1000) and HRP-conjugated Beta Actin (2D4H5) monoclonal antibody (HRP-66009,

(15) Guide-specific loss of efficiency and off-target reduction with Cas9 variantsbioRxivMarch 21, 2023Liang Zhang, Wei He, Rongjie Fu, Han Xufollowed by blocking with 5% non-fat milk and incubation with primary antibody against Cas9 (CST, #14697, 1:2,000), YAP1 (CST, #14074, 1:2,000), MTAP (CST, #4158, 1:2,000) or β-actin (Sigma, #A5316, 1:10,000).

(16) CRISPR-Cas9 genetic screen leads to the discovery of L-Moses, a KAT2B inhibitor that attenuates Tunicamycin-mediated neuronal cell deathScientific reportsMarch 9, 2023Pavlou S, Foskolou S, Patikas N, Field SF, Papachristou EK, Santos C, Edwards AR, Kishore K, Ansari R, Rajan SS, et al.were used to enrich for nuclear proteins. PVDF membranes were incubated overnight with 1/1000 Cas9 (#14697, Cell Signalling)18, 1/2000 B-Actin (#ab8227, Abcam)19, 1/10,000 GAPDH (Abcam, ab128915)20, 1/100 KAT2B

(17) Development of a CRISPRi Human Retinal Pigmented Epithelium Model for Functional Study of Age-Related Macular Degeneration GenesInternational journal of molecular sciencesFebruary 8, 2023Wang JH, Urrutia-Cabrera D, Lees JG, Mesa Mora S, Nguyen T, Hung SSC, Hewitt AW, Lim SY, Edwards TL, Wong RCB.0.1% Triton X-100 (Sigma-Aldrich). The samples were then immunostained with antibodies against Cas9 (#14697, Cell Signaling, Danvers, United States) or ZO-1 (#339100, Thermo Fisher), Alexa Fluor 488 secondary

(18) A Molecular Switch between Mammalian MLL Complexes Dictates Response to Menin–MLL InhibitionCancer DiscoveryJanuary 9, 2023Yadira M. Soto-Feliciano, Francisco J. Sánchez-Rivera, Florian Perner, Douglas W. Barrows, Edward R. Kastenhuber, Yu-Jui Ho, Thomas S Carroll, Yijun Xiong, Disha Anand, Alexey A. Soshnev, et al.antibodies (GE Healthcare). Primary antibodies used included anti-Cas9 (Cell Signaling Technology, 14697), anti-UTX (Cell Signaling Technology, 33510), anti-Menin (Bethyl, A300-105A), anti-NF-YA (Santa Cruz Biotechnology

(19) Genome Editing Using Cas9 Ribonucleoprotein Is Effective for Introducing PDGFRA Variant in Cultured Human Glioblastoma Cell LinesInternational Journal of Molecular SciencesDecember 28, 2022Taiji Hamada, Seiya Yokoyama, Toshiaki Akahane, Kei Matsuo, Akihide Tanimotoanti-PDGFRβ monoclonal antibody (#3169; Cell Signaling Technology), mouse anti-Cas9 monoclonal antibody (#14697; Cell Signaling Technology), and rabbit anti-β-actin antibody (#4970; Cell Signaling Technology). 4.4.

(20) A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem CellsGenes (Basel)December 14, 2022Shawna L. Jurlina, Melissa K. Jones, Devansh Agarwal, Diana V. De La Toba, Netra Kambli, Fei Su, Heather M. Martin, Ryan Anderson, Ryan M. Wong, Justin Seid, et al.and rinsed 3 times with PBS for 5 min each. A mouse Cas9-specific monoclonal antibody (clone 7A9-3A3 #14697; Cell Signaling Technology, Danvers, MN, USA) was diluted at 1:800 in PBS containing 2% horse serum

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(1)

6/22/2022Good Western Blot Antibody for Cas9 Protein Marta BaroWe transduced a Cas9 plasmid in multiple cancer cell lines. We used this antibody to validate the expression of Cas9 in these cell lines using Western blot technique. We used this antibody to screen ... Read Review
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3/2/2020Great Antibody to Detect Full-Length Cas9 Scott ScarneoThis product worked well and was reliable.

Cas9 ( S. pyogenes ) (7A9-3A3) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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Cas9 ( S. pyogenes ) (7A9-3A3) Mouse mAb from Cell Signaling Technology

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