Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190 from Abcam

Product Specs
ItemMouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab170190
Quantity100 µg
ApplicationsELISA, Flow Cyt, ICC/IF, IHC-P
Concentration1 mg/ml
Conjugate/TagUnconjugated
IsotypeIgG1
HostMouse
ClonalityMonoclonal
Clone15-6E10A7
Gene Nameccdc113
Antibody TypeIsotype Control Antibody

Questions and Answers

Can this isotype control antibody be used in flow cytometry experiments?

Yes, the Mouse IgG1, kappa monoclonal [15-6E10A7] isotype control has been utilized in flow cytometry applications, as indicated in various studies where it served as a control for specific antibody staining.

Is this antibody suitable for use in immunohistochemistry?

The Mouse IgG1, kappa monoclonal [15-6E10A7] has been shown to be effective in immunohistochemistry, as referenced in studies where it was employed as an isotype control to validate specific antibody staining in tissue sections.

What species does this isotype control antibody react with?

This isotype control antibody is reactive with human samples, specifically Homo sapiens, making it suitable for experiments involving human tissues or cells.

How has this antibody been utilized in scientific research?

The Mouse IgG1, kappa monoclonal [15-6E10A7] has been cited in multiple scientific articles, indicating its application in various experimental contexts, including studies on antigen presentation and immune responses, where it served as a control to ensure the specificity of other antibodies used in the assays.

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Company Profile

Website: http://www.abcam.com

(1) Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repairBioact MaterApril 21, 2026Feng Lin, Lufeng Yao, Yiwen Xu, Lei Xiang, Ning Zhang, Yaping Zhuang, Qinzhe Jiang, Zengjie Zhang, Qihua Cao, Jiayu Chen, et al.ab275977, Abcam, UK; ab30394, Abcam, UK) for 30 min at room temperature. The IgG isotype control antibody (ab170190, Abcam, UK) was used at the same concentration and conditions as the primary antibody. The secondary

(2) Mechanism of N6-methyladenosine (m6A)-mediated upregulation of LINC00958 in the growth of cervical cancerFront OncolMarch 6, 2026Yuting Li, Zhengying Liu, Hongling Jinfollowed by ethanol precipitation and collection. Anti-m6A antibody (ab208577, Abcam) or anti-IgG (ab170190, Abcam) was incubated overnight with Protein A/G beads in IP buffer (150 mM NaCl, 0.1% NP-40, 10 mM

(3) Dental pulp stem cells and hydroxyapatite: a promising combination for maxillary bone regenerationBMC Oral HealthFebruary 12, 2026Nur Atmaliya Luchman, Rohaya Megat Abdul Wahab, Shahrul Hisham Zainal Ariffin, Farinawati Yazid, Nurrul Shaqinah Nasruddin, Seng Fong Laumouse and rabbit-specific HRP/DAB IHC Detection Kit Micro-Polymer (ab236466, Abcam, UK). Isotype-matched control antibodies, including mouse IgG1 (ab170190, Abcam, UK) and rabbit IgG (ab37415, Abcam, UK), were included at the same concentration as the primary

(4) Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cellsFrontiers in Bioengineering and BiotechnologyJanuary 12, 2026Monika Buhl, Arkadiusz Jundziłł, Paweł Dąbrowski, Kamil Szeliski, Marta Rasmus, Tomasz Kloskowski, Łukasz Kaźmierski, Tomasz Drewa, Marta Pokrywczyńska"Mouse IgG1, kappa monoclonal [15-6E10A7]-isotype control",FC: 2.5 μg/ml,"Abcam, Cambridge, United Kingdom, Cat# ab170190" Rabbit monoclonal antibody against CK14,IF: 1.1 μg/ml,"Abcam, Cambridge, United Kingdom, Cat# ab181595" Rabbit

(5) SERSµDrop: A Platform to Study Cell–Cell Communication via SERS ImagingSmallNovember 18, 2025Paula Piñeiro, Judith Langer, Joaquin Seras‐Franzoso, Dorleta Jimenez de Aberasturi, Sara Abalde‐Cela, Malou Henriksen‐Lacey, Luis M. Liz‐MarzánThermoFisher. AF488 goat anti‐mouse IgG (ab150117), AF405 donkey anti‐mouse IgG (ab175659) and IgG isotype (ab170190) control were purchased from Abcam. Matrigel were purchased from Corning. All chemicals were used without

(6) Regulation of fibroblast-like synoviocyte function by cadherin 6 in rheumatoid arthritisArthritis Res TherAugust 29, 2025Camilla R. L. Machado, Eunice Choi, Narayanan B. Perumal, Robert J. Benschop, Gregory R. Dressler, Wei Wang, David L. Boyle, Gary S. Firesteinnegative controls (no primary antibody, polyclonal rabbit IgG control, and mouse IgG control (Abcam, ab170190)). After washing, anti-Rabbit IgG Alexa Fluor 594 and anti-Mouse IgG Alexa Fluor 488 (Invitrogen, A-11012,

(7) miR-424/322 attenuates cardiac remodeling by modulating the nuclear factor-activated T-cell 3/furin pathwayBiomedical journalAugust 1, 2025Tsai HY, Wang JC, Hsu YJ, Lin CY, Huang PH, Tsai MC, Hsu CW, Yang SF, Tsai SH.at room temperature. Exogenous TGF-β1 (PeproTech. Cat#100–21) and mouse IgG1 isotype control (Abcam, ab170190) were employed as positive and negative controls, respectively. Finally, the cells were mounted using

(8) KIAA1429 promotes non-small-cell lung cancer cell proliferation through the TRERNA1/HOXA6 axisSci RepJuly 10, 2025Yunxia Li, Shiyao Tian, Biao Yangin RIP lysis buffer. The cell lysates were incubated with a negative control immunoglobulin G (IgG, ab170190/ab172730, Abcam) or target antibodies at 4 °C overnight to prepare the immunoprecipitated protein-RNA

(9) Association of eosinophil major basic protein with intraocular lens dislocation in atopic dermatitisSci RepMay 15, 2025Koji Komatsu, Yoichiro Masuda, Hoshiho Kubota, Ai Iwauchi, Masanobu Iida, Kosuke Ichihara, Euido Nishijima, Naoki Yamamoto, Masayuki Shimoda, Tadashi NakanoDojindo Laboratory, Kumamoto, Japan) was used. For each specimen, mouse IgG1κ monoclonal antibody (ab170190, Abcam, Cambridge, UK) was used as an isotype control (negative control). An isotype control was used

(10) Molecular mechanism of ZC3H13 -mediated ferroptosis in doxorubicin resistance of triple negative breast cancerCell Biol ToxicolFebruary 26, 2025Li Huang, Lei Han, Shuai Liang, Guohui Hanfragmented at 70℃ for 5 min and then incubated with 3 μg m6A antibody (ab208577, Abcam) or IgG antibody (ab170190, Abcam) at 4℃ for 6 h. After washing, the enriched RNA was eluted for analysis. RNA immunoprecipitation

(11) Protocol for isolating CD163+ Kupffer cells from human liver resectionsSTAR ProtocolsDecember 20, 2024Armando Andres Roca Suarez, Marie-Laure Plissonnier, Maud Michelet, Anaëlle Dubois, Sarah Heintz, Maria Saez-Palma, Marion Delphin, Isabelle Bordes, Jennifer Molle, Audrey Diederichs, et al.μg/mL),Abcam,ab955 Rabbit IgG Ctrl (dilution: 5 μg/mL),Abcam,ab171870 Mouse IgG Ctrl (dilution: 1 μg/mL),Abcam,ab170190 "Anti-rabbit Alexa 488 (dilution: 1/1,000)",Cell Signaling Technology,4412S Anti-mouse Alexa 594 (dilution:

(12) Human rhinovirus 16 induces an ICAM-1-PKR-ATF2 axis to modulate macrophage functionsJournal of VirologyOctober 22, 2024Suzanne Faure-Dupuy, Manon Depierre, Zoé Fremont-Debaene, Floriane Herit, Florence Niedergangincubated with an antibody against human ICAM-1 (Biotechne, BBA3) or a control IgG antibody (Abcam, ab170190) at a final concentration of 10 µg/mL for 1 h before and during the infection with HRV. Then, cells

(13) DNA methylation, but not microRNA expression, is affected by in vitro THC exposure in bovine granulosa cellsBMC Pharmacology and ToxicologyJuly 15, 2024Sabrina Floccari, Reem Sabry, Laurie Choux, Michael S. Neal, Jibran Y. Khokhar, Laura A. Favettaisotype control for 5-mC was added, Mouse igG1, kappa monoclonal isotype control [15-6E10A7] (Abcam ab170190) (1:1000 in PBS + 0.1% Triton™ X-100 + 5% BSA). The no-stain control was incubated in PBS + 0.1% Triton™

(14) Calprotectin Impairs Platelet Survival in Patients With Primary Antiphospholipid SyndromeArthritis & RheumatologyJune 1, 2024Claire K. Hoy, Somanathapura K. NaveenKumar, Sherwin A. Navaz, Kavya Sugur, Srilakshmi Yalavarthi, Cyrus Sarosh, Tristin Smith, Katarina Kmetova, Emily Chong, Noah F. Peters, et al.calprotectin (2–10 μg/mL) were treated with a 1:1 ratio of 27E10 (ab17050, Abcam)/Isotype control IgG (ab170190, Abcam) for 1 hour before treating with purified healthy platelets. After the serum incubation, platelets

(15) Cross species systems biology discovers glial DDR2, STOM, and KANK2 as therapeutic targets in progressive supranuclear palsyNature CommunicationsNovember 2, 2023Yuhao Min, Xue Wang, Özkan İş, Tulsi A. Patel, Junli Gao, Joseph S. Reddy, Zachary S. Quicksall, Thuy Nguyen, Shu Lin, Frederick Q. Tutor-New, et al.hour on ice. Concurrently, mouse IgG1, kappa monoclonal [15-6E10A7] isotype was included as controls (ab170190, Abcam, 1:50). Goat anti-mouse Alexa488 secondary antibodies (ab150113, Abcam, 1:200) were incubated

(16) Ionizing radiation normalizes the features of active breast cancer stromal fibroblasts and suppresses their paracrine pro-carcinogenic effectsTranslational oncologyNovember 1, 2023Aboussekhra A, Alraouji NN, Al-Mohanna FH, Al-Khalaf H.described [18]. Antibodies directed against, α-SMA (ab5694), TGF-β1 (ab-64,715), SDF-1 (ab-10,395), Twist1 (ab170190), IL-6 (ab-9324), Lamin B1 (ab8982) and Vimentin (ab-8978) were purchased from Abcam (Cambridge, MA).

(17) The m6A reader YTHDC1-mediated lncRNA CTBP1-AS2 m6A modification accelerates cholangiocarcinoma progressionHeliyonSeptember 4, 2023Zhe Jin, Yahui Liumagnetic beads pre-coated with 10 μg of anti-m6 A antibody (ab208577, Abcam, UK) or anti-mouse IgG (ab170190, Abcam, UK). N6-methyladenosine 5′-monophosphate sodium salt (6.7 mM) was applied to elute the m6 A-modified

(18) Bio-Enhanced Neoligaments Graft Bearing FE002 Primary Progenitor Tenocytes: Allogeneic Tissue Engineering & Surgical Proofs-of-Concept for Hand Ligament Regenerative MedicinePharmaceuticsJuly 3, 2023Jeannerat A, Meuli J, Peneveyre C, Jaccoud S, Chemali M, Thomas A, Liao Z, Abdel-Sayed P, Scaletta C, Hirt-Burri N, et al.control antibody: Abcam Ref. N°ab172730 (1:100 dilution)Mouse isotype control antibody: Abcam Ref. N°ab170190 (1:100 dilution)Secondary anti-rabbit Alexa 488 antibody: Abcam Ref. N°ab150081 (1:250 dilution)Secondary

(19) Dynamic interactome of the MHC I peptide loading complex in human dendritic cellsProceedings of the National Academy of SciencesJune 20, 2023Martina Barends, Nicole Koller, Christian Schölz, Verónica Durán, Berislav Bošnjak, Jennifer Becker, Marius Döring, Hanna Blees, Reinhold Förster, Ulrich Kalinke, et al.either with 15 µg purified TAP1 antibody (mAb 148.3) or with 15 µg isotype control antibody (Abcam, ab170190). Samples were incubated for 3 h at 4 °C on an overhead rotator. Afterward, beads were washed three

(20) METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cellsCell Death DiscoveryDecember 1, 2022Renbing Jiang, Zhibing Dai, Junshen Wu, Suzhi Ji, Yachao Sun, Wenpeng Yangextracted using TRIzol reagent. Then, RNA was incubated with anti-m6A (ab208577, Abcam) or anti-IgG (ab170190, Abcam) and protein A/G magnetic beads in IP buffer overnight. After ethanol precipitation, the immunoprecipitated

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Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control from Abcam

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Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control from Abcam

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