Fig 1: MiR‐10b‐3p inhibition suppressed cell growth and motility and enhanced radiosensitivity in ESCA cells by targeting RHCG. (a) The protein level of RHCG in EC109 and KYSE150 cells transfected with si‐NC or si‐RHCG was measured by western blot assay. (b) MiR‐10b‐3p expression in EC109 and KYSE150 cells with anti‐NC or anti‐miR‐10b‐3p transfected was detected by qRT‐PCR. (c–j) EC109 and KYSE150 cells were transfected with anti‐NC+si‐NC, anti‐miR‐10b‐3p+si‐NC or anti‐miR‐10b‐3p+si‐RHCG. (c–g) The proliferation, migration, invasion and survival fraction of EC109 and KYSE150 cells were estimated by EdU, wound‐healing, transwell and colony formation assays, respectively. (h) The apoptosis of EC109 and KYSE150 cells under radiation exposure was analyzed by flow cytometry analysis. (i and j) The protein levels of bcl‐2, bax and c‐caspase3 in EC109 and KYSE150 cells upon radiation were examined via western blot assay. *p < 0.05, **p < 0.01
Fig 2: CircATIC overexpression inhibited tumorigenesis and promoted radiosensitivity in vivo. (a) The bodyweight in different groups was examined. (b and c) Tumor volume and weight were measured. (d) The expression of Ki67, RHCG and c‐caspase3 in xenograft tumors was examined. **p < 0.01
Fig 3: CircATIC/miR‐10b‐3p/RHCG affected ESCA cell progression. (a) The protein level of RHCG in EC109 and KYSE150 cells transfected with vector+miR‐NC, oe‐circATIC+miR‐NC or oe‐circATIC+miR‐10b‐3p was determined by western blot assay. (b) A schematic diagram of circATIC in regulating ESCA progression. **p < 0.01
Fig 4: RHCG was targeted by miR‐10b‐3p. (a) The target genes of miR‐10b‐3p were predicted by Targetscan and GEPIA. (b) Low expression of RHCG in ESCA. (c) The mRNA level of RHCG in ESCA tissues and normal tissues was detected by qRT‐PCR. (d and e) The mRNA level of RHCG in ESCA patients with different TNM stages, positive and negative lymph node metastasis was detected by qRT‐PCR. (f and g) The protein level of RHCG in tumor tissues and cells was measured via western blot assay. (h) The binding sites between RNCG and miR‐10b‐3p. (i–l) The interaction between miR‐10b‐3p and RHCG was demonstrated by dual‐luciferase reporter assay. (m) The protein level of RHCG in EC109 and KYSE150 cells transfected with miR‐NC or miR‐10b‐3p was measured by western blot assay. *p < 0.05, **p < 0.01
Supplier Page from Abcam for Anti-RHCG antibody