Fig 2: RRS1 was efficiently knockdown in human colon cancer cells(A-B) Quantitative RT-PCR and Western blot analysis revealed the RRS1 expression was efficiently knockdown in the RKO cells. (C-D) Quantitative RT-PCR and Western blot analysis revealed the RRS1 expression was efficiently knockdown in the HCT116 cells. ***P<0.001.

Fig 3: RRS1 is frequently expressed in human HCC tissues and cell lines. (A) PCR analysis of RRS1 expression in human liver cancer cell lines SMMC-7721, HepG2, Huh-7, and Hep3B. (B) Expression of RRS1 mRNA in 18 cases of HCC and adjacent tissues. N, paracancerous tissue and T, cancerous tissue. Patient cancer tissues were directly compared to the matched adjacent tissues. The levels of RRS1 mRNA in the tissues were detected by RT-qPCR. The smaller the value of ∆Ct, the higher the expression level.

Fig 4: Expression and localization of RRS1 in breast cancer cells. (A) and (B) protein expression of RRS1 in normal human breast epithelial cells and breast cancer cell lines. (C) The mRNA content of RRS1 in normal human breast epithelial cells and breast cancer cell lines as detected by quantitative PCR. (D) The localization of RRS1 in BT549 cells as detected by immunofluorescence. ****P<0.0001. RRS1, regulator of ribosome synthesis 1.

Fig 5: Disruption of multiple key pathways are involved in colon cancer cells after RRS1 knockdown(A) Heat map representation of 905 genes (324 genes up-regulated and 581 genes down-regulated) showed significant differential expression patterns in HCT116 cells infected with lentivirus expressing shCtrl and shRRS1 (criteria P<0.05, absolute fold change >1.5). (B) Functional pathway enrichment of differential genes was analyzed using IPA software. (C) Interactional network was constructed between genes involved in p53 pathway. Blue circles represent down-regulated genes, red circles represent up-regulated and genes of gray circles represent no expression changing. (D) The expression of indicated downstream targets of p53 signaling. (E) The expression of MKI67, CDC25C, CDK1, CDKN1A and p53 that was examined by Western blot assay. GAPDH is used as an internal control.