Fig 1: Relations between DIAPHs expression and prognosis of PAAD in TCGA database. (A–B) Overall survival and disease-free survival analyses between the DIAPHs high-expression and low-expression cohort. High-cutoff (50%) and low-cutoff (50%) values were used as the expression thresholds for separating the high-expression and low-expression cohorts, in log-rank test. HR, hazard ratio. (C) AUC of DIAPH1, DIAPH2, and DIAPH3 expression for PAAD. AUC, area under ROC curve. (D) DIAPHs as risk factors in nomograms for overall survival and progression-free survival in patients with PAAD.
Fig 2: DIAPH3 knockdown suppressed cell proliferation. Stable DIAPH3 knockdown cells, MG-63-shDIAPH3 and HOS-shDIAPH3, and negative control (NC) cells, MG-63-NC and HOS-NC, were harvested. DIAPH3 expression level in MG-63-NC, HOS-NC, MG-63-shDIAPH3, and HOS-shDIAPH3 cells was measured by qRT-PCR (A) and western blotting (B). B, on the left are representative images of western blotting. On the right is the statistical evaluation of relative DIAPH3 protein expression, expressed as a relative ratio of the densitometric value of DIAPH3 protein to the densitometric value of GAPDH. The OD450 nm value of above group cells at 0, 24, 48, and 72 h was ascertained using a CCK8 assay (C). The effect of DIAPH3 knockdown on cell cycle was evaluated using flow cytometry analysis (D). *P < 0.05, when compared to NC group
Fig 3: mRNA expression levels of DIAPHs in pan-cancer. (A) DIAPH1 expression. (B) DIAPH2 expression. (C) DIAPH3 expression. Log2 (TPM+1) transformed the expression data for plotting. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, in Wilcoxon test. TPM, transcripts per million; N and T, normal and tumor tissues.
Fig 4: DIAPH3 knockdown suppressed cell migration and invasion. Stable DIAPH3 knockdown cells, MG-63-shDIAPH3 and HOS-shDIAPH3, and negative control (NC) cells, MG-63-NC and HOS-NC, were harvested. The effect of DIAPH3 knockdown on cell migration (A, B) and invasion (C) was evaluated using Transwell or wound healing assays. A representative image is shown on the left. Statistical evaluation of migrating cell number and percentage of wound closure or invaded cell number is shown on the right. *P < 0.05, when compared to NC group
Fig 5: DIAPH3-related gene network, KEGG pathway analysis, and GO enrichment analysis. (A) A total of 50 available experimentally determined DIAPH3-interacting proteins using STRING tool. (B)An intersection analysis of the DIAPH3-interacting and -correlated genes. (C) The correlation between DIAPH3 expression and four intersected genes expression in PAAD. *** p < 0.001, in Spearman’s rho. TPM, transcripts per million. (D) GO enrichment and KEGG pathway analyses based on the DIAPH3-interacting and -correlated genes. Adjusted p-values were obtained from multiple hypotheses test using the Benjamini–Hochberg procedure; p. adjust <0.05 was considered statistically significant. (E) Correlation between DIAPH3 and intersected four genes expression in pan-cancer, in purity-adjusted partial Spearman’s rho.
Supplier Page from Abcam for Anti-DIAPH3 antibody