Notch-1 Antibody AF5267 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemNotch-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF5267
ApplicationsIHC-F,Flow Cytometry,Immunohistochemistry,Western Blot
IsotypeIgG
HostSheep
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
ReactivityHamster,Human,Mouse
TargetNotch-1
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse Notch-1, Ala19-Gln526, Accession # AAM28905
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namenotch receptor 1
NCBI Gene AliasesAOS5, AOVD1, TAN1, hN1

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Notch-1 in direct ELISAs and Western blots. In direct ELISA, approximately 10% cross-reactivity with recombinant human Notch-1 and recombinant rat Notch-1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Notch-2 and rmNotch-3 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) In vivo evidence for GDP-fucose transport in the absence of transporter SLC35C1 and putative transporter SLC35C2The Journal of biological chemistryDecember 1, 2023Lu L, Varshney S, Yuan Y, Wei HX, Tanwar A, Sundaram S, Nauman M, Haltiwanger RS, Stanley P.

(2) EOGT enables residual Notch signaling in mouse intestinal cells lacking POFUT1Scientific reportsOctober 14, 2023Nauman M, Varshney S, Choi J, Augenlicht LH, Stanley P.°C. Flow cytometry performed within 1–6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems,

(3) Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cellsThe Journal of biological chemistryJuly 1, 2022Matsumoto K, Kumar V, Varshney S, Nairn AV, Ito A, Pennarubia F, Moremen KW, Stanley P, Haltiwanger RS.(eBioscience; catalog no.: #17-0081, Clone:53-6.7, 1:200), and anti-N1 Ab (R&D Systems; catalog no.: #AF5267, 1:50) was added. After incubation for 30 min at 4 °C in the dark, 1 ml LBB was added, cells were pelleted,

(4) Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2The Journal of biological chemistryOctober 23, 2020Kakuda S, LoPilato RK, Ito A, Haltiwanger RS.

(5) Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migrationBMC neuroscienceApril 29, 2019Wiszniak S, Schwarz Q.rabbit anti-N1ICD, 1:100 (Cell Signaling Technologies 4147); sheep anti-Notch1, 1:200 (R&D Systems AF5267). Antigen retrieval Immunostaining for AP2α and N1ICD required antigen retrieval [44]. Cryosections

(6) Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signalingProceedings of the National Academy of Sciences of the United States of AmericaSeptember 4, 2018Takeuchi H, Schneider M, Williamson DB, Ito A, Takeuchi M, Handford PA, Haltiwanger RS.solution, pH 7.4 (Gibco)]. Cells were incubated with 100 μL of anti-mN1 (ECD) antibody (R&D Systems, AF5267) at 10 μg/mL for 1 h at 4 °C. Cells were washed with binding buffer and then incubated with PE–anti-sheep

(7) Inhibition of Delta-induced Notch signaling using fucose analogsNature chemical biologyJanuary 1, 2018Schneider M, Kumar V, Nordstrøm LU, Feng L, Takeuchi H, Hao H, Luca VC, Garcia KC, Stanley P, Wu P, et al.solution pH 7.4 (Gibco)). Cells were incubated with 100 μl of anti-mN1 (ECD) antibody (R&D systems, AF5267) at 10 μg/ml for 1 h at 4 °C. Cells were washed with binding buffer and then incubated with PE-anti-sheep

(8) Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1Nature communicationsSeptember 18, 2017Wang Y, Wu B, Lu P, Zhang D, Wu B, Varshney S, Del Monte-Nieto G, Zhuang Z, Charafeddine R, Kramer AH, et al.µl ligand binding buffer containing 7.5 µg/ml DLL4-Fc or anti-hamster NOTCH1 antibody (R&D Systems, AF5267, 1:50 in ligand binding buffer) at 4 °C for 1 h. Soluble DLL4-Fc ligand was prepared as described previously66.

(9) O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammalseLifeApril 11, 2017Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, et al.ECD antibody (8G10, Santa Cruz [sc-32756]), sheep anti-hamster NOTCH1 ECD antibody (R and D Systems, AF5267), rabbit anti-human NOTCH1 ECD antibody (H-131, Santa Cruz [sc-9170]), rabbit anti-human NOTCH1 ICD

(10) Cell-Cell Contact Area Affects Notch Signaling and Notch-Dependent PatterningDevelopmental cellMarch 13, 2017Shaya O, Binshtok U, Hersch M, Rivkin D, Weinreb S, Amir-Zilberstein L, Khamaisi B, Oppenheim O, Desai RA, Goodyear RJ, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,, anti-mouse-Notch1 (1:100),R&D Systems,AF5267; RRID: AB_1655874 Alexa Fluor 488-conjugated AffiniPure Rabbit-anti-Sheep (1:200),Jackson ImmunoResearch,313-545-003;

(11) Lunatic, Manic, and Radical Fringe Each Promote T and B Cell DevelopmentJournal of immunology (Baltimore, Md. : 1950)January 1, 2016Song Y, Kumar V, Wei HX, Qiu J, Stanley P.clone H1.2F3; FoxP3-APC rat clone FJK-16s; antigen-purified polyclonal sheep anti-NOTCH1 (aa 19–526) AF5267 (R&D Systems, Minneapolis, MN); rabbit polyclonal IgG anti-NOTCH2 (aa 25–255) sc-5545 (Santa Cruz, Dallas,

(12) Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteinsThe Journal of biological chemistryApril 18, 2014Tashima Y, Stanley P.

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Mouse Notch-1 Antibody

Notch-1 Antibody

Notch-1 Antibody

Notch-1 Antibody

Notch-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (12)

Reviews

Notch-1 Antibody from R&D Systems, a Bio-Techne Brand

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