Complement Factor D/Adipsin Antibody AF5430 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemComplement Factor D/Adipsin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $469.00Supplier Page View Company Product Page
Catalog NumberAF5430
HostSheep
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetComplement Factor D/Adipsin
ReactivityMouse
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Complement Factor D/Adipsin, Ile26-Ser259, Accession # P03953
NCBI Full Gene Namecomplement factor D (adipsin), complement factor D
NCBI Gene AliasesADN, DF, ADIPSIN, Adn, PFD
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.

Sizes Available: 100 ug (also 25 ug)

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Targeting adipocyte ESRRA alleviates osteoarthritis via interrupting inter-organelle crosstalk of complement C3-CFD-MAC cascadeBone ResApril 29, 2026Tongling Huang, Zihui Wang, Lu Gao, Jun Gao, Zhaocheng Lu, Pengda Li, Chon Him Choy, Zhuolei Yuan, Yanting Zhong, Chang-An Geng, et al.Cell Signaling Technology #13826), Tubulin (1:5 000, BPI #AbM59005-37B-PU), mouse CFD (1:800, R&D Systems #AF5430), human CFD (ABclonal #A23006), C3 (1:5 000, ABclonal #A13283), GAPDH (1:5 000, Proteintech #60004-1-Ig),

(2) CD9 Counteracts Liver Steatosis and Mediates GCGR Agonist Hepatic EffectsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)August 1, 2024Zheng Y, Wang Y, Xiong X, Zhang L, Zhu J, Huang B, Liu X, Liu J, Zhu Z, Yang G, et al.(SA35‐08) and anti‐PGC1α (NBP1‐04676) were obtained from Novus. The anti‐CFD was obtained from R&D (AF5430). The anti‐FLI1 was obtained from GeneTex (GTX112937). The anti‐NOX4 (14347‐1‐AP), anti‐ACC1 (21923‐1‐AP),

(3) PPARγ Acetylation in Adipocytes Exacerbates BAT Whitening and Worsens Age-Associated Metabolic DysfunctionCellsMay 18, 2023He Y, Zhang R, Yu L, Zahr T, Li X, Kim TW, Qiang L.Rosemont, IL, USA, catalog # 13171-1-AP), anti-Adipsin (R&D systems, Minneapolis, MN, USA, catalog # AF5430), anti-Adiponectin (Invitrogen, Waltham, MA, USA, catalog # PA1-054), anti-PPARγ (Cell Signaling Technology,

(4) Uncoupling Lipid Synthesis from Adipocyte DevelopmentBiomedicinesApril 9, 2023Wan Q, Calhoun C, Zahr T, Qiang L.for western blotting analysis. Antibodies of Adipsin (R&D Systems, Minneapolis, MN, USA; catalogue AF5430) and Adiponectin (Thermo Fisher Scientific, Waltham, MA, USA, catalogue PA1-054) were used in this study.

(5) PPARγ Acetylation Orchestrates Adipose Plasticity and Metabolic RhythmsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)January 1, 2023He Y, B'nai Taub A, Yu L, Yao Y, Zhang R, Zahr T, Aaron N, LeSauter J, Fan L, Liu L, et al.ThermoFisher Scientific, #PA1‐523), anti‐ubiquitin (Proteintech, #10201‐2‐AP), anti‐adipsin (R&D systems, #AF5430), anti‐C3 (Proteintech, #21337‐1‐AP), anti‐GAPDH (Proteintech, #HRP‐60004), anti‐HSP90 (Proteintech,

(6) Acetylation of PPARγ in macrophages promotes visceral fat degeneration in obesityLife MetabDecember 1, 2022Aaron N, Zahr T, He Y, Yu L, Mayfield B, Pajvani UB, Qiang L.Scientific). Antibodies used for western blot analysis were as follows: anti-Adipsin (R&D Systems, catalog #AF5430), anti-Adiponectin (Invitrogen, catalog # PA1-054), and anti-HSP90 (Proteintech,catalog #1371-1-AP). Luciferase

(7) Selective targeting of visceral adiposity by polycation nanomedicineNature nanotechnologyDecember 1, 2022Wan Q, Huang B, Li T, Xiao Y, He Y, Du W, Wang BZ, Dakin GF, Rosenbaum M, Goncalves MD, et al.

(8) LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammationeLifeNovember 8, 2022Choi CHJ, Barr W, Zaman S, Model C, Park A, Koenen M, Lin Z, Szwed SK, Marchildon F, Crane A, et al.RRID:AB_1079276",Western blot (1:5000) Antibody,Anti-CFD/Adipsin (sheep polyclonal),R and D Systems,"Cat# AF5430, RRID:AB_1655868",Western blot (1:1000) Antibody,Anti-Cytochrome c (rabbit monoclonal),Cell Signaling Technology

(9) Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell InteractionCancersAugust 23, 2021Mizuno M, Khaledian B, Maeda M, Hayashi T, Mizuno S, Munetsuna E, Watanabe T, Kono S, Okada S, Suzuki M, et al.(No. CTKN001, KAC, Kyoto, Japan), filters were incubated with an anti-mouse adipsin (1:400, Clone: AF5430, R&D Systems, Minneapolis, NM, USA) or an HRP conjugated anti-β-actin (1:5000, Clone: AC-15, Sigma)

(10) Adipsin promotes bone marrow adiposity by priming mesenchymal stem cellseLifeJune 22, 2021Aaron N, Kraakman MJ, Zhou Q, Liu Q, Costa S, Yang J, Liu L, Yu L, Wang L, He Y, et al.Scientific). Antibodies used for western blot analysis were as follows: anti-Adipsin (R&D Systems, catalog# AF5430), anti-adiponectin (Invitrogen, catalog# PA1-054), anti-C3 (Proteintech, catalog# 21337-1-AP), anti-FABP4

(11) Cell-Type-Specific Complement Expression in the Healthy and Diseased RetinaCell reportsNovember 26, 2019Pauly D, Agarwal D, Dana N, Schäfer N, Biber J, Wunderlich KA, Jabri Y, Straub T, Zhang NR, Gautam AK, et al.Germany)","#341272 / (Schäfer et al., 2017); RRID:AB_2082392" sheep anti-CFD,"R&D Systems (Minneapolis, MN, USA)",#AF5430; RRID:AB_1655868 mouse anti-C5,"Quidel (San Diego, CA, USA)",#A217; RRID:AB_452484 goat anti-C6,"Complement

(12) Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from LipodystrophyJournal of immunology (Baltimore, Md. : 1950)April 15, 2018Wu X, Hutson I, Akk AM, Mascharak S, Pham CTN, Hourcade DE, Brown R, Atkinson JP, Harris CA.

(13) Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse RetinaPloS oneJanuary 1, 2016Williams JA, Stampoulis D, Gunter CE, Greenwood J, Adamson P, Moss SE.(product no. A311; Quidel, 1:500), polyclonal sheep immunoglobulin (IgG) to mouse CFD (product no. AF5430; R&D Systems, 1:500) and goat antisera to human C5 (product no. A306; Quidel, 1:500). Secondary antibodies

(14) C3 dysregulation due to factor H deficiency is mannan-binding lectin-associated serine proteases (MASP)-1 and MASP-3 independent in vivoClinical and experimental immunologyApril 1, 2014Ruseva MM, Takahashi M, Fujita T, Pickering MC.for 1 h with either a polyclonal affinity-purified goat anti-mouse FD antibody (2·8 μg, product no. AF5430; R&D Systems, Abingdon, UK) or an immunoglobulin (IgG) fraction of polyclonal goat anti-mouse C3 (2·8

(15) Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsisJournal of immunology (Baltimore, Md. : 1950)December 1, 2013Zou L, Feng Y, Li Y, Zhang M, Chen C, Cai J, Gong Y, Wang L, Thurman JM, Wu X, et al.

Be the First to Write a Review!

Complement Factor D/Adipsin Antibody

Complement Factor D/Adipsin Antibody

Complement Factor D/Adipsin Antibody

Complement Factor D/Adipsin Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (15)

Reviews

Complement Factor D/Adipsin Antibody from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map