Human IgG Isotype Control 02-7102 from Thermo Fisher Scientific

Product Specs
ItemHuman IgG Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number02-7102
Concentration5 mg/mL
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
Antibody TypeIsotype Control
Storage-20°C, Avoid Freeze/Thaw Cycles
ClonalityPolyclonal
ApplicationsFlow Cytometry (FCM)
HostHuman
IsotypeIgG
TargetHuman IgG
FormatPBS with 0.1% sodium azide; pH 7.4
Conjugate/TagUnconjugated
Quantity10 mg

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Caspase-activation powers anti-Desmoglein 3-induced acantholysis in human epidermisCell Death DiscoveryFebruary 19, 2026Morna F. Schmidt, Maria A. Feoktistova, Diana Panayotova-Dimitrova, Eva Miriam Buhl, Peter Boor, Tim Ruhl, Jens Waschke, Ritva Tikkanen, Martin Röcken, Jens M. Baron, et al.Darmstadt, Hessen). The following stimuli and reagents were used: the IgG human isotype control (# 02-7102, Thermo Fisher Scientific Inc., Waltham, USA), Dulbecco´s Phosphate Buffered Saline (DPBS, P04-36500,

(2) Enhancement of drug delivery through fibroblast activation protein–targeted near-infrared photoimmunotherapyJCI InsightDecember 22, 2025Seitaro Nishimura, Kazuhiro Noma, Tasuku Matsumoto, Yasushige Takeda, Tatsuya Takahashi, Hijiri Matsumoto, Kento Kawasaki, Hotaka Kawai, Tomoyoshi Kunitomo, Masaaki Akai, et al.Abraxane, panitumumab, or isotype. Sibrotuzumab (1 mg; TAB-211, Creative Biolabs) and the isotype control (02-7102, Thermo Fisher Scientific) were mixed with a 5-fold molar excess of IRDye700DX N-hydroxysuccinimide

(3) Conversion of natural cytokine receptors into orthogonal synthetic biosensorsNature chemical biologyNovember 1, 2025Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN.µl of fresh FACS buffer and 10 µl of human IgG (human IgG isotype control; Thermo Fisher Scientific, 02-7102, RRID: AB_2532958; stock concentration 1 mg ml−1) were added. Cells were incubated in this mixture at

(4) EGFR activation in cholangiocytes promotes extrahepatic bile duct regeneration after injuryHepatol CommunOctober 14, 2025Ashley N. Calder, Takuki Sakaguchi, Mirabelle Peter, John Tobias, Timothy Frankel, Nataliya Razumilava10 μg/mL trastuzumab (MedChemExpress, Cat#: HY-P9907) 21 or a human IgG isotype (Invitrogen, Cat#: 027102) control was added to cells 24 hours after seeding and replaced every 2 days until the final analysis. Immunohistochemistry Mouse

(5) Spatially-restricted inflammation-induced senescent-like glia in multiple sclerosis and patient-derived organoidsNature CommunicationsSeptember 26, 2025Francesca Fagiani, Edoardo Pedrini, Maria Sofia Martire, Gaia Gastoldi, Colin Vanden Bulcke, Jing-Ping Lin, Dragan Maric, Elena Brambilla, Francesca Ruffini, Carolina Peri, et al.#300-02-100 μg], and 400 ng/mL C1q [Mybiosource, #MBS147305) for 24 h; (4) immunoglobulins IgG (Invitrogen, #02-7102) at the concentration of 8 μL/mL for 24 h. For drug treatments, after 6 h-treatment with a pool of 6

(6) First-generation and preclinical evaluation of an EphA5-targeted antibody-drug conjugate in solid tumorsJ Clin InvestJuly 15, 2025Fernanda I. Staquicini, Fenny H.F. Tang, Vanessa de Oliveira, Sun-Young Kim, Ethan R. Chen, Christopher Markosian, Daniela I. Staquicini, Yongjian Wu, J. Kellogg Parsons, Kirstin F. Barnhart, et al.custom humanized anti-EphA5 primary antibody or an isotype control (ThermoFisher Scientific; catalog 02-7102) at 4°C for 30 minutes. After the incubation, cells were washed twice with FACS buffer and resuspended

(7) Placental cytotrophoblast microvillar stabilization is required for cell-cell fusionDevelopmentApril 1, 2025Wendy K. Duan, Sumaiyah Z. Shaha, Juan F. Garcia Rivas, Bethan L. Wilson, Khushali J. Patel, Ivan K. Domingo, Meghan R. Riddellpermeabilized in blocking buffer (5% normal donkey serum, 0.5% Triton-X100, 1:100 human IgG (Invitrogen, 02-7102, lot WI327305). Tissue was then incubated overnight at 4°C in blocking buffer with primary antibodies.

(8) EphA2 regulates vascular permeability and prostate cancer metastasis via modulation of cell junction protein phosphorylationOncogeneFebruary 1, 2025Offenhäuser C, Dave KA, Beckett KJ, Smith FM, Jayakody BA, Cooper LT, Agyei-Yeboah H, McCarron JK, Li Y, Bastick K, et al.human IgG1Fc; custom production by CSL) or Fc control proteins (human IgG; Thermo Fisher Scientific #02-7102) were clustered with Fc fragment-specific rabbit anti-human secondary antibodies (Jackson ImmunoResearch

(9) HaloTag display enables quantitative single‐particle characterisation and functionalisation of engineered extracellular vesiclesJournal of Extracellular VesiclesJuly 5, 2024Roxana E. Mitrut, Devin M. Stranford, Beth N. DiBiase, Jonathan M. Chan, Matthew D. Bailey, Minrui Luo, Clare S. Harper, Thomas J. Meade, Muzhou Wang, Joshua N. LeonardFACS buffer (50 µL) and blocked with 10 µL of 1 mg/mL IgG (Thermo Fisher, Human IgG Isotype Control, 02–7102, RRID: AB_2532958) for 5 min at 4°C. Next, FLAG‐tag antibody (Bio‐Techne, DYKDDDDK Epitope Tag Alexa Fluor® 488‐conjugated Antibody

(10) HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesiclesbioRxiv : the preprint server for biologyMay 3, 2024Mitrut RE, Stranford DM, DiBiase BN, Chan JM, Bailey MD, Luo M, Harper CS, Meade TJ, Wang M, Leonard JN.FACS buffer (50 μL) and blocked with 10 μL of 1 mg/mL IgG (Thermo Fisher, Human IgG Isotype Control, 02-7102, RRID: AB_2532958) for 5 min at 4°C. Next, FLAG-tag antibody (Bio-Techne, DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody

(11) Building Synthetic Biosensors Using Red Blood Cell ProteinsACS Synthetic BiologyApril 19, 2024Taylor B. Dolberg, Taylor F. Gunnels, Te Ling, Kelly A. Sarnese, John D. Crispino, Joshua N. Leonardfresh FACS buffer was added, and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02–7102, RRID: AB_2532958, stock concentration 1 mg/mL) was added. Cells were incubated at 4°C for 5 min. 2.5

(12) An exhausted-like microglial population accumulates in aged and APOE4 genotype Alzheimer’s brainsImmunityJanuary 9, 2024Alon Millet, Jose Henrique Ledo, Sohail F. Tavazoieintraperitoneally with 0.5mg of either aducanumab (Cardinal Health) or human IgG isotype control (Invitrogen #02–7102) daily for 5 days prior to immune cell isolation and library prep as for the 10-week-old and 20-week-old

(13) Genetically encoding multiple functionalities into extracellular vesicles for the targeted delivery of biologics to T cellsNature Biomedical EngineeringNovember 27, 2023Devin M. Stranford, Lacy M. Simons, Katherine E. Berman, Luyi Cheng, Beth N. DiBiase, Michelle E. Hung, Julius B. Lucks, Judd F. Hultquist, Joshua N. Leonardwas decanted, and cells were resuspended in 50 μL of FACS buffer. 10 μL of human IgG (Thermo Fisher 027102) was added, cells were flicked to mix, and were incubated at 4°C for 5 min. Conjugated primary antibody

(14) Perturbations in podocyte transcriptome and biological pathways induced by FSGS associated circulating factorsAnnals of Translational MedicineJune 30, 2023Priyanka Rashmi, Tara K. Sigdel, Dmitry Rychkov, Izabella Damm, Andrea Alice Da Silva, Flavio Vincenti, Andre L. Lourenco, Charles S. Craik, Jochen Reiser, Minnie M. Sarwalpodocytes were treated with patient sera in the presence of a control antibody (human IgG, Invitrogen #02-7102) or anti-uPAR antibody (2G10) or anti-CD40 antibody (BMS, 986090) at 1 µg/mL for 24 h. The 2G10 antibody

(15) DOG1 as a novel antibody-drug conjugate target for the treatment of multiple gastrointestinal tumors and liver metastasisFrontiers in immunologyJanuary 1, 2023Wu Y, Li W, Chen X, Wang H, Su S, Xu Y, Deng X, Yang T, Wei M, Li L, et al.medium were trypsinized, washed and incubated with anti-DOG1 antibodies or human IgG isotype control (02-7102, Invitrogen) at a concentration of 10 μg/ml at 37°C for 45 min. The samples were washed and incubated

(16) Stabilization of the SARS-CoV-2 Receptor Binding Domain by Protein Core Redesign and Deep Mutational ScanningbioRxivMay 26, 2022Alison C. Leonard, Jonathan J. Weinstein, Paul J. Steiner, Annette H. Erbse, Sarel J. Fleishman, Timothy A. WhiteheadBackground binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02–7102). RBD designs were produced recombinantly in Pichia pastoris as follows. pPICZα vectors (ThermoFisher

(17) Elucidating Design Principles for Engineering Cell-Derived Vesicles to Inhibit SARS-CoV-2 InfectionSmall (Weinheim an der Bergstrasse, Germany)May 1, 2022Gunnels TF, Stranford DM, Mitrut RE, Kamat NP, Leonard JN.FACS buffer (50 µL) and blocked with 10 µL of 1 mg mL−1 IgG (Thermo Fisher, Human IgG Isotype Control, 02‐7102, RRID: AB_2532958) for 5 min at 4 °C. After blocking, 2.5 µL of 0.2 µg µL−1 α‐ACE2 antibody (R&D Systems,

(18) Pitfalls in the Application of Dispase-Based Keratinocyte Dissociation Assay for In Vitro Analysis of Pemphigus VulgarisVaccines (Basel)January 28, 2022Morna F. Schmidt, Maria Feoktistova, Diana Panayotova-Dimitrova, Ramona A. Eichkorn, Amir S. Yazdi[20])Human immunoglobulin isotype control (Thermo Fisher Scientific Inc., Waltham, MA, USA, Cat. no. 02-7102) 2.2. Reagents and Equipment 24-Well Cell Culture Multiwell Plate(sterile, with lid) (Greiner AG,

(19) Elucidating design principles for engineering cell-derived vesicles to inhibit SARS-CoV-2 infectionbioRxiv : the preprint server for biologyDecember 10, 2021Gunnels TF, Stranford DM, Mitrut RE, Kamat NP, Leonard JN.50 μL FACS buffer and blocked with 10 μL of 1 mg/mL IgG (Thermo Fisher, Human IgG Isotype Control, 02–7102, RRID: AB_2532958) for 5 min at 4°C. After blocking, 2.5 μL of 0.2 μg/μL α-ACE2 antibody from R&D Systems

(20) Targeted biologic inhibition of both tumor cell-intrinsic and intercellular CLPTM1L/CRR9-mediated chemotherapeutic drug resistanceNPJ precision oncologyMarch 2, 2021Parashar D, Geethadevi A, McAllister D, Ebben J, Peterson FC, Jensen DR, Bishop E, Pradeep S, Volkman BF, Dwinell MB, et al.where indicated to account for any effect of diluent constituents. Normal mouse IgG (Thermo Fisher, 02-7102) was used as a non-specific antibody control for monoclonal antibody treatment where indicated. Rabbit

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Human IgG Isotype Control from Thermo Fisher Scientific

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Human IgG Isotype Control from Thermo Fisher Scientific

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