TNF alpha Antibody AMC3012 from Thermo Fisher Scientific

Product Specs
ItemTNF alpha Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberAMC3012
Storage Temperature4° C
Antibody TypePrimary
Concentration1 mg/mL
Storage4°C
PurityProtein A
ReactivityHu, Ms
ClonalityPolyclonal
NCBI Gene AliasesTNFalpha, TNLG1F, DIF, Tnfsf1a, TNF-a, Tnfa, TNFSF2, TNF-alpha, Tnlg1f, TNFA
NCBI Full Gene Nametumor necrosis factor
ApplicationsWB, ELISA, ICC, Neut
ImmunogenRecombinant mouse TNF-alpha produced in E. coli
HostRabbit
IsotypeIg
TargetTNF alpha
FormatPBS with 125mM trehalose and no preservative
Conjugate/TagUnconjugated
Quantity500 µg

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Effects of surface-exposed sialic acid and LOS outer core on the ability of native Neisseria meningitidis outer membrane vesicles (nOMVs) to induce cytokine expression and pyroptotic pathways in THP-1-derived macrophagesScientific ReportsDecember 1, 2025Silvia Caterina Resta, Adelfia Talà, Antonio Baccante, Giovanni Saudino, Pasquale Petruccelli, Vito Di Cioccio, Cecilia Bucci, Pietro Alifanoanti-gasdermin E (Abcam, ab215191), anti-gasdermin D (Cell Signaling Technology, #39754), anti-TNF-α (Invitrogen, #AMC3012) and anti-IL1β (Invitrogen, #P420B). Anti-tubulin (Santa Cruz Biotechnology, sc-23948) was used as a

(2) Intralipid Infusion to Fetal Sheep ( Ovis aries ) Promotes Differentiation and Lipid Accumulation of Adipose TissuesFASEB JNovember 15, 2025Xinrui Li, Sarah M. Alaniz, Samantha Louey, Jeanene Marie Deavila, Sonnet S. Jonker, Min Duanti‐PGC‐1α (1:1000 dilution, #20658–1‐AP, Proteintech, Rosemont, IL), anti‐TNFα (1:1000 dilution, #AMC3012, Invitrogen), anti‐PDGFRα (1:1000 dilution, #5241, Cell Signaling Technology), anti‐p65 (1:1000 dilution,

(3) Intralipid Infusion to Fetal Sheep ( Ovis aries ) Promotes Differentiation and Lipid Accumulation of Adipose TissuesFASEB JOctober 29, 2025Xinrui Li, Sarah M. Alaniz, Samantha Louey, Jeanene Marie Deavila, Sonnet S. Jonker, Min Duanti‐PGC‐1α (1:1000 dilution, #20658–1‐AP, Proteintech, Rosemont, IL), anti‐TNFα (1:1000 dilution, #AMC3012, Invitrogen), anti‐PDGFRα (1:1000 dilution, #5241, Cell Signaling Technology), anti‐p65 (1:1000 dilution,

(4) Dysregulated inflammation, oxidative stress, and protein quality control in diabetic HFpEF: unraveling mechanisms and therapeutic targetsCardiovascular diabetologyMay 14, 2025Delalat S, Sultana I, Osman H, Sieme M, Zhazykbayeva S, Herwig M, Budde H, Kovács Á, Kaçmaz M, Göztepe E, et al.Antibody,Product NO,Company,Dilution TNF-α,AMC3012,Invitrogen,1:500 IL-6,Sc.32296,Santa Cruz Biotechnology,1:1000 IL-6 Receptor,MA5-29721,Invitrogen,1:1000 eNOS,32027,Cell Signaling Technology

(5) Response Gene to Complement 32 is associated with poor patient survival and an inflamed tumor-immune microenvironment in clear cell renal cell carcinomaTranslational oncologyFebruary 1, 2025Li L, Bu X, Wang S, Liu Y, Chen C, Zhang W, Zhao P.the neutralized antibodies (NAbs), 1ug/ml anti-IL-1β (Cat: P420B, Invitrogen) and anti-TNF-α (Cat: AMC3012, Invitrogen), and then were used to stimulate renal cancer cells. shRNA transfection and plasmid construct Renal cancer

(6) The antiosteoporotic effect of oxymatrine compared to testosterone in orchiectomized ratsJ Orthop Surg ResJanuary 9, 2025Anwaar M. Shaban, Eman A. Ali, Sara G. Tayel, Sara Kamal Rizk, Dalia F. El Agamyconducted. Then, the sections were treated with a primary TNF-α antibody (rabbit polyclonal antibody AMC3012, Thermo Fisher Scientific, USA), followed by the addition of a biotinylated goat-polyvalent secondary

(7) Macrophage-Hepatocyte Circuits Mediated by Grancalcin Aggravate the Progression of Metabolic Dysfunction Associated SteatohepatitisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 1, 2024Su T, He Y, Wang M, Zhou H, Huang Y, Ye M, Guo Q, Xiao Y, Cai G, Zhao M, et al.For neutralizing antibody experiments, the concentrations of anti‐TNFα (Thermo Fisher Scientific,Cat#AMC3012), anti‐IL‐1β (Thermo Fisher Scientific,Cat#16‐7012‐81), and anti‐IL‐6 (Thermo Fisher Scientific,Cat#16‐7061‐81)

(8) Human 3D Ovarian Cancer Models Reveal Malignant Cell-Intrinsic and -Extrinsic Factors That Influence CAR T-cell ActivityCancer researchAugust 1, 2024Joy JD, Malacrida B, Laforêts F, Kotantaki P, Maniati E, Manchanda R, Annibaldi A, Hopkins S, Garrobo-Calleja I, Gautrot J, et al.

(9) Tolerance to Haemophilus influenzae infection in human epithelial cells: Insights from a primary cell-based modelPLoS pathogensJuly 1, 2024Kappler U, Henningham A, Nasreen M, Yamamoto A, Buultjens AH, Stinear TP, Sly P, Fantino E.Sigma-Aldrich, 1:2000), NGAL (PA5115502, ThermoFisher, 1:500), IL-8 (PA579113, Thermo Fisher, 1:500), TNFα (AMC3012, ThermoFisher, 1:1000), an Anti-Rabbit–Alexa conjugate antibody (A27041, ThermoFisher, 1:10 000) was

(10) A modified Mediterranean-style diet enhances brain function via specific gut-microbiome-brain mechanismsGut MicrobesMarch 6, 2024Gwoncheol Park, Saurabh Kadyan, Nathaniel Hochuli, Julie Pollak, Bo Wang, Gloria Salazar, Paramita Chakrabarty, Philip Efron, Julia Sheffler, Ravinder Nagpalpurchased from Invitrogen, Waltham, MA, USA), including IL-1β (cat# P420B), IL-6 (cat# P620), TNF-α (cat# AMC3012), claudin-5 (cat# 35–2500) zonulin-1 (cat# 40–2200), GPR81 (cat# PA5–114741), Aβ (cat# 51–2700), total-tau

(11) Protein Kinase D Plays a Crucial Role in Maintaining Cardiac Homeostasis by Regulating Post-Translational Modifications of Myofilament ProteinsInternational journal of molecular sciencesFebruary 28, 2024Herwig M, Begovic M, Budde H, Delalat S, Zhazykbayeva S, Sieme M, Schneider L, Jaquet K, Mügge A, Akin I, et al.UK)",ab19534,1:1000 IL-6,"Invitrogen (Waltham, MA, USA)",P620,1:1000 IL-18,Invitrogen,PA5-80719,1:1000 TNF-α,Invitrogen,AMC3012,1:1000 NF-κB p65,Cell Signaling Technology,8242S,1:1000 Phospho-NF-κB p65 (S536),Cell Signaling Technology,3033S,1:1000 Myosin-binding

(12) Neutrophil extracellular traps aggravate intestinal epithelial necroptosis in ischaemia-reperfusion by regulating TLR4/RIPK3/FUNDC1-required mitophagyCell proliferationJanuary 1, 2024Chu C, Wang X, Chen F, Yang C, Shi L, Xu W, Wang K, Liu B, Wang C, Sun D, et al.IL‐1β (P420B, Thermo Fisher, China), human & mouse IL‐6 (M620, Thermo Fisher), human & mouse TNF‐α (AMC3012, Thermo Fisher) and human & mouse MCP‐1 (MA5‐17040, Thermo Fisher) in serum or intestinal tissue were

(13) Neisseria meningitidis activates pyroptotic pathways in a mouse model of meningitis: role of a two-partner secretion systemFrontiers in cellular and infection microbiologyJanuary 1, 2024Pagliuca C, Colicchio R, Resta SC, Talà A, Scaglione E, Mantova G, Continisio L, Pagliarulo C, Bucci C, Alifano P, et al.(Cell Signaling Technology, #9662), IL-1β (Invitrogen, #P420B), IL-18 (abcam, ab207323) or TNF-α (Invitrogen, #AMC3012). Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-mouse monoclonal antibody

(14) LILRB2/PirB mediates macrophage recruitment in fibrogenesis of nonalcoholic steatohepatitisNature communicationsJuly 22, 2023Li DP, Huang L, Kan RR, Meng XY, Wang SY, Zou HJ, Guo YM, Luo PQ, Pan LM, Xiang YX, et al.

(15) Pyruvate Dehydrogenase Kinase 1 inhibition mediated oxidative phosphorylation enhancement in cartilage promotes osteoarthritis progressionBMC musculoskeletal disordersJuly 20, 2023Yang X, Jiang Q, Luan T, Yu C, Liu Z, Wang T, Wan J, Huang J, Li K.collagen (BS-10589R, Bioss), aggrecan (BA2967-1, Boster), F4/80 (14,480,181, eBioscience) and TNF-a (AMC3012, Thermo) 14 h at 4℃, followed by subsequent experiments according to the ABC-HRP kit instruction (PK4001,

(16) Thymol in Trachyspermum ammi seed extract exhibits neuroprotection, learning, and memory enhancement in scopolamine-induced Alzheimer's disease mouse modelPhytotherapy research : PTRJuly 1, 2023Timalsina B, Haque MN, Choi HJ, Dash R, Moon IS.

(17) Endometriosis leads to central nervous system-wide glial activation in a mouse model of endometriosisJournal of neuroinflammationMarch 6, 2023Bashir ST, Redden CR, Raj K, Arcanjo RB, Stasiak S, Li Q, Steelman AJ, Nowak RA.AB5541), rabbit anti-interleukin (IL)-6 (1:250; Abcam, ab208113), rabbit anti-TNF (1:500; ThermoFisher, AMC3012), and rabbit anti-KI67 (1:2,000; Abcam, ab15580). Primary and secondary antibody controls were used

(18) Oxidative stress and inflammation distinctly drive molecular mechanisms of diastolic dysfunction and remodeling in female and male heart failure with preserved ejection fraction ratsFrontiers in cardiovascular medicineJanuary 1, 2023Zhazykbayeva S, Hassoun R, Herwig M, Budde H, Kovács Á, Mannherz HG, El-Battrawy I, Tóth A, Schmidt WE, Mügge A, et al.,Cell Signaling,"1:1,000" Nox2,MA5-35348,Invitrogen,"1:1,000" Nox4,MA5-32090,Invitrogen,"1:1,000" SQSTM1/P62,39749S,Cell Signaling,"1:1,000" TnF alpha,AMC3012,Invitrogen,"1:1,000" GAPDH,G9545-200UL,Sigma,"1:10,000" Primary antibody list.

(19) Characteristic Features of Deep Brain Lymphatic Vessels and Their Regulation by Chronic StressResearch (Washington, D.C.)January 1, 2023Chang J, Guo B, Gao Y, Li W, Tong X, Feng Y, Abumaria N.anti-IL-1β rabbit antibody (1:1,000, Abcam, #ab9722), anti-TNF-α rabbit antibody (1:3,000, Invitrogen, #AMC3012), anti-VEGF-C mouse antibody (1:1,000, Arigo, #ARG56212, China), anti-VEGFR3 rabbit antibody (1:1,000,

(20) Isolation of DiNP-Degrading Microbes from the Mouse Colon and the Influence DiNP Exposure Has on the Microbiota, Intestinal Integrity, and Immune Status of the ColonToxicsFebruary 6, 2022Chiu KK, Bashir ST, Abdel-Hamid AM, Clark LV, Laws MJ, Cann I, Nowak RA, Flaws JA.anti-IL-6 (ab208113, 1:500), anti-MUC1 (Invitrogen PA5-95487, 1:50), and anti-TNF alpha (Invitrogen AMC3012, 1:1000). The following day, slides were washed, incubated with the appropriate secondary antibody,

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3/23/2021TNF Alpha Antibody Works Well for Immunofluorescence Xiaoguang LiWe used this TNF alpha antibody to detect TNF alpha in cell lines or in mice tissue sections (skin tissue) using immunofluorescence.

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TNF alpha Antibody from Thermo Fisher Scientific

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TNF alpha Antibody from Thermo Fisher Scientific

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