Quantitative immunofluorescence of MIC-1 in human prostate tissues.(A-D) MIC-1 expression levels (indicated as signal intensities [pixel count]) in disease-free, tumor adjacent, and tumor tissues; the types of analysis were the following (as per Materials and Methods): (A and B) Whole slide analysis (WSA) for all (A) and non-matched (B) cases in the UNMH/CHTN cohort; (C and D) region of interest (ROI) analysis for all (C) and non-matched (D) cases in the UNMH/CHTN cohort. Individual data points are shown as small black squares (partially overlapping); the boxes represent group medians (line across middle) and quartiles (25th and 75th percentiles) at its ends; lines above and below boxes indicate 10th and 90th percentiles, respectively. For each analysis, the number of images and cases is indicated; p values above the panels denote the level of statistical significance for the differences between groups, as calculated by the student’s t-test (p(t)) and by the Wilcoxon rank sums test (p(WRS)).