Phospho-c-Raf (Ser621) Antibody 44-504G from Thermo Fisher Scientific

Product Specs
ItemPhospho-c-Raf (Ser621) Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number44-504G
ModificationsPhosphorylated: Ser621
Storage Temperature-20°C
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C
ReactivityHuman (Hu)
ClonalityPolyclonal
NCBI Gene AliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
NCBI Full Gene NameRaf-1 proto-oncogene, serine/threonine kinase
ApplicationsWB, IP
ImmunogenThe antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains serine 621. The sequence is conserved in mouse and rat
HostRabbit
IsotypeIgG
TargetPhospho-c-Raf (Ser621)
FormatDulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
Conjugate/TagUnconjugated
Quantity100 µL

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) RAF1 promotes successful human cytomegalovirus replication and is regulated by AMPK-mediated phosphorylation during infectionJournal of VirologyMarch 18, 2025Diana M. Dunn, Ludia J. Pack, Joshua C. Munger,Cell Signaling Technology,9427 Phospho-Raf1 (S621),Abcam,ab4767 Phospho-Raf1 (c-Raf) (Ser621),Thermo Fisher Scientific,44–504G Raf1 (c-Raf) (D4B3J),Cell Signaling Technology,53745 Phospho-MEK1/2 (Ser217/S221),Cell Signaling Technology,8211 MEK1/2

(2) Oncogenic non-V600 mutations evade the regulatory machinery of RAF including the Cdc37/Hsp90 chaperone and the 14-3-3 scaffoldTheranosticsJanuary 6, 2025Xiaoyu Wan, Jiajun Yap, Junjun Chen, Yifan Li, Regina Faruk, Nazereth Chor Boon Tan, Yiying Ma, Yiting Lim, Karlina Bte Jubri, Jingyi Hu, et al.anti-ERK1/2 (sc-514302) and anti-BRAF (sc-5284) (Santa Cruz Biotechnology); anti-phospho-CRAF (Ser621) (44504G, Invitrogen); anti-Ki67 (ab16667, Abcam); and HRP-labeled secondary antibodies (#31460, #31430, Invitrogen).

(3) Echinacoside Induces Mitochondria-Mediated Pyroptosis through Raf/MEK/ERK Signaling in Non-Small Cell Lung Cancer CellsJournal of immunology researchJanuary 1, 2022Shi Y, Cao H, Liu Z, Xi L, Dong C.anti-p-ERK1/2 [ab201015, 1 : 1000, Abcam], anti-Raf [PA5-29333, 1 : 2000, Invitrogen], anti-p-Raf [44-504G, 1 : 1000, Invitrogen], anti-MEK1/2 [ab178876, 1 : 20000, Abcam], anti-p-MEK1/2 [ab278564, 1 : 1000,

(4) 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia CellsPloS oneJanuary 1, 2015Bunaciu RP, Jensen HA, MacDonald RJ, LaTocha DH, Varner JD, Yen A.polyclonal IgG (unconjugated) #2729 (for FRET),were from Cell Signaling (Danvers, MA). The pS621c-Raf (44504G) rabbit antibody (for WB) was from Invitrogen. The goat anti-rabbit IgG (H+L) Alexa Fluor 594 conjugated

(5) Heterogeneous effects of calorie restriction on in vivo glucose uptake and insulin signaling of individual rat skeletal musclesPloS oneJanuary 1, 2014Sharma N, Sequea DA, Castorena CM, Arias EB, Qi NR, Cartee GD.were from Cell Signaling Technology (Danvers, MA). Anti-phospho-IR Tyr1162/1163 (pIRTyr1162/1163; #44–504G) was from Invitrogen (Camarillo, CA). Anti-sheep IgG horseradish peroxidase conjugate (#12–342) was

(6) Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscleBiochimica et biophysica actaNovember 1, 2012Sharma N, Arias EB, Sequea DA, Cartee GD.were from Cell Signaling Technology (Danvers, MA). Anti-phospho IR Tyr1162/1163 (pIRTyr1162/1163; #44-504G) and anti-IR (#AHR0271) were from Invitrogen (Camarillo, CA). Anti-AS160 (#07-741), anti-GLUT4 (#CBL243)

(7) Mechanisms for increased insulin-stimulated Akt phosphorylation and glucose uptake in fast- and slow-twitch skeletal muscles of calorie-restricted ratsAmerican journal of physiology. Endocrinology and metabolismJune 1, 2011Sharma N, Arias EB, Bhat AD, Sequea DA, Ho S, Croff KK, Sajan MP, Farese RV, Cartee GD.

(8) Nitric oxide increases p21(Waf1/Cip1) expression by a cGMP-dependent pathway that includes activation of extracellular signal-regulated kinase and p70(S6k)The Journal of biological chemistryApril 14, 2000Gu M, Lynch J, Brecher P.

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Phospho-c-Raf (Ser621) Antibody from Thermo Fisher Scientific

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Phospho-c-Raf (Ser621) Antibody from Thermo Fisher Scientific

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