Lola29M/F molecular actions. a-hrobo1 promoter-luciferase reporter assays. a The robo1 genomic map (upper) and reporter schematics (lower). The genomic distance (in kb) is indicated on the top with the reference point 0, at which the robo1 genomic fragment was fused with the luciferase-coding sequence (blue box with an arrow). Below the thick line, the exon (box)-intron (thin line) organization of robo1 is shown. Filled and open portions of the box represent the coding and non-coding regions, respectively. b The relative activities (ordinate) of a 4.1 kb robo1-promoter reporter cotransfected without (-) or with (+) Lola29M, FruBM or both. c The activities of reporters (ordinate) carrying a robo1-promoter fragment of different lengths (the fragment length is indicated on the abscissa) with (filled bars) or without (open bars) cotransfection of Lola29M or truncation-resistant Lola29M[K41R]. d The reporter activities were unaffected by cotransfection with Lola29F-like (Lola29M[Δ1-300]). e The suppression effect of Lola29M on reporter activities was inhibited by Lola29M[Δ1-300]. f-m Lola29M binding to the robo1 promoter. f Genomic fragments used for EMSA. Probe DNA B and Probe DNA B ∆DR1 are shown in an expanded scale in the second and third rows. Reporters are schematically illustrated at the bottom. Pal 1: palindrome sequence, FROS: the FruBM-binding region, DR1: direct repeat 1. g The sequence around DR1 and FROS. The deletion in the 0.9 kb reporter is also i