GST Tag Antibody 71-7500 from Thermo Fisher Scientific

Product Specs
ItemGST Tag Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number71-7500
Quantity100 µg
ApplicationsELISA, Immunocytochemistry, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 0.1% sodium azide; pH 7.4
ReactivityTag
TargetGST Tag
IsotypeIgG
HostRabbit
ImmunogenPurified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pGEX vector
ClonalityPolyclonal
Concentration0.25 mg/mL
Storage Temperature-20°C
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) p53 regulates DREAM complex-mediated repression in a p21-independent mannerThe EMBO JournalMarch 4, 2025Ritu Agrawal, Sagar Sengupta5017, RRID: AB_10693385 (WB 1:4000)" Rabbit anti-GST (used for WB),Thermo Fisher Scientific,"Cat# 71-7500, RRID: AB_2533994 (WB 1:5000)" Oligonucleotides and other sequence-based reagents,Oligonucleotides

(2) Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b releaseJournal of cell scienceNovember 1, 2024Min KW, Choi KM, Mun H, Ko S, Lee JW, Sagum CA, Bedford MT, Kim YK, Delaney JR, Cho JH, et al.min. For detection of all possible proteins, the chips were then incubated with rabbit anti-GST antibody (71-7500, Invitrogen) at 1:5000 for 1 h at room temperature, washed three times for 15 min in RNase-free PBS

(3) DNA sequence and chromatin differentiate sequence-specific transcription factor binding in the human malaria parasite Plasmodium falciparumNucleic Acids ResearchSeptember 23, 2024Victoria A Bonnell, Yuning Zhang, Alan S Brown, John Horton, Gabrielle A Josling, Tsu-Pei Chiu, Remo Rohs, Shaun Mahony, Raluca Gordân, Manuel Llináschecked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution]. The homeodomain from HDP1 was tagged and purified similarly with modifications

(4) Evidence for an interaction of paracingulin with microtubulesmicroPublication biologyJanuary 1, 2024Flinois A, Mutero-Maeda A, Montessuit S, Citi S.the following proteins were used at the indicated dilution for immunoblotting (IB): rabbit GST tag (71-7500; Thermo Fisher Scientific; IB: 1/2000); mouse β-tubulin (32-2600; Thermo Fisher Scientific; IB: 1/3500)

(5) Deterministic early endosomal maturations emerge from a stochastic trigger-and-convert mechanismNature communicationsAugust 2, 2023York HM, Joshi K, Wright CS, Kreplin LZ, Rodgers SJ, Moorthi UK, Gandhi H, Patil A, Mitchell CA, Iyer-Biswas S, et al.8 µg/mL recombinant GST-2xFYVE70 which was detected using a GST primary antibody at 1:500 dilution(71–7500, Invitrogen) and a Goat anti-Rabbit AlexaFluor647 secondary antibody (A-21245, Thermo Fisher Scientific)

(6) PI(4,5)P(2)-dependent regulation of endothelial tip cell specification contributes to angiogenesisScience advancesMarch 31, 2023Davies EM, Gurung R, Le KQ, Roan KTT, Harvey RP, Mitchell GM, Schwarz Q, Mitchell CA.(322600), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; am4300), glutathione S-transferase (GST; 71-7500), Alexa Fluor 488–conjugated phalloidin (A12379), Ki67 (RM-9106-S0), 4′,6-diamidino-2-phenylindole (DAPI),

(7) A Broad-Based Characterization of a Cell-Penetrating, Single Domain Camelid Bi-Specific Antibody Monomer That Targets STAT3 and KRAS Dependent CancersInternational journal of molecular sciencesJuly 8, 2022Singh S, Murillo G, Richner J, Singh SP, Berleth E, Kumar V, Mehta R, Ramiya V, Parihar AS.(Jackson ImmunoResearch, West Grove, PA, USA, Cat. No. 711-545-152), rabbit polyclonal anti-GST: (Thermo Fisher #71-7500, Waltham, MA, USA). The blocking solution, 1° and 2° antibody diluent were 1% BP: (1% BSA in PBS). All

(8) Selective endocytosis of Ca²⁺-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticityScience advancesMay 27, 2022Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, et al.(Cell Signaling Technology, catalog no. 4207; RRID:AB_1264223), GST tag (Thermo Fisher Scientific, catalog no. 71-7500; RRID:AB_2533994), HA tag (GeneTex, catalog no. GTX115044; RRID:AB_10622369), HIP1 (Abcam, catalog no.

(9) Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAPNature communicationsMarch 4, 2022García-García M, Sánchez-Perales S, Jarabo P, Calvo E, Huyton T, Fu L, Ng SC, Sotodosos-Alonso L, Vázquez J, Casas-Tintó S, et al.

(10) Structural basis of the human Scribble-Vangl2 association in health and diseaseThe Biochemical journalApril 16, 2021How JY, Stephens RK, Lim KYB, Humbert PO, Kvansakul M.polyclonal anti-β-PIX antibody (4515S; Cell Signaling Technology) and rabbit polyclonal anti-GST antibody (71-7500; Invitrogen). © 2021 The Author(s). This is an open access article published by Portland Press Limited

(11) WW, PH and C-Terminal Domains Cooperate to Direct the Subcellular Localizations of PLEKHA5, PLEKHA6 and PLEKHA7Frontiers in cell and developmental biologyJanuary 1, 2021Sluysmans S, Méan I, Jond L, Citi S.(Harvard Medical School),R40.76 RRID: AB_2205518 Rabbit polyclonal anti-GST,Thermo Fisher Scientific,Cat# 71-7500 RRID: AB_2533994 Alexa Fluor 488-AffiniPure Donkey anti-Rabbit IgG,Jackson ImmunoResearch,"Cat# 711-545-152,

(12) Toll-like receptor 2 stimulation augments esophageal barrier integrityAllergyDecember 1, 2019Ruffner MA, Song L, Maurer K, Shi L, Carroll MC, Wang JX, Muir AB, Spergel JM, Sullivan KE.(trimethylated H3 lysine 4 ,Active Motif 39159), and anti-GST (glutathione-S-transferase, ThermoFisher 71-7500) antibodies were used for immunoprecipitation. Antibody-bound complexes were collected, eluted and extracted

(13) An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered CellsMolecular CellJuly 1, 2019Yoshiki Narimatsu et al."Goat anti-Human IgG (H+L), Alexa Fluor™ 647",Invitrogen,Cat#A-21445 GST tag polyclonal antibody,Invitrogen,Cat#71-7500 "Goat anti-Rabbit IgG (H+L), Alexa Flouor™ 647",Invitrogen,CatA-21245 NUH2 antibody,"Nudelman et al.,

(14) SUMOs Mediate the Nuclear Transfer of p38 and p-p38 during Helicobacter Pylori InfectionInternational journal of molecular sciencesAugust 22, 2018Wang PY, Hsu PI, Wu DC, Chen TC, Jarman AP, Powell LM, Chen A.(#2032, Cell Signaling), rabbit anti-RFP (632496, Clontech, Mountain View, CA, USA), rabbit anti-GST (71-7500, Life Technologies), rabbit anti-His (SC-803, Santa Cruz Biotechnology, Dallas, TX, USA), horseradish

(15) The major leaf ferredoxin Fd2 regulates plant innate immunity in ArabidopsisMolecular plant pathologyJune 1, 2018Wang M, Rui L, Yan H, Shi H, Zhao W, Lin JE, Zhang K, Blakeslee JJ, Mackey D, Tang D, et al.

(16) Small GTPase Rab8a-recruited Phosphatidylinositol 3-Kinase γ Regulates Signaling and Cytokine Outputs from Endosomal Toll-like ReceptorsThe Journal of biological chemistryMarch 17, 2017Wall AA, Luo L, Hung Y, Tong SJ, Condon ND, Blumenthal A, Sweet MJ, Stow JL.

(17) SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophagesNature communicationsJanuary 18, 2017Luo L, Bokil NJ, Wall AA, Kapetanovic R, Lansdaal NM, Marceline F, Burgess BJ, Tong SJ, Guo Z, Alexandrov K, et al.

(18) C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less OrganellesCellOctober 20, 2016Lee KH, Zhang P, Kim HJ, Mitrea DM, Sarkar M, Freibaum BD, Cika J, Coughlin M, Messing J, Molliex A, et al.anti-SRSF7 (11044-1-AP, Proteintech), anti-Flag (F7425, Sigma), anti-His (ab18184, Abcam), and anti-GST (71-7500 or MA4-004, Thermo). Species-specific IRDye® secondary antibodies were used and visualized using Odyssey®

(19) PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3eLifeMay 25, 2016Chung HR, Xu C, Fuchs A, Mund A, Lange M, Staege H, Schubert T, Bian C, Dunkel I, Eberharter A, et al.The interactions were evaluated by SDS-PAGE and detected using a GST specific antibody (Invitrogen 71– 7500). GST Pull downs Nuclei were prepared from HeLa in a hypotonic buffer (10 mM Tris HCl pH 7.4, 10 mM

(20) Rab8a interacts directly with PI3Kγ to modulate TLR4-driven PI3K and mTOR signallingNature communicationsJuly 15, 2014Luo L, Wall AA, Yeo JC, Condon ND, Norwood SJ, Schoenwaelder S, Chen KW, Jackson S, Jenkins BJ, Hartland EL, et al.anti-Rab8 (610845) was purchased from BD Transduction Laboratories (Lexington, KY, USA). GST antibody (71-7500) was purchased from Invitrogen, Sydney, Australia. Anti-TLR4 for western blotting was from Abcam (ab22048).

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GST Tag Antibody from Thermo Fisher Scientific

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