Rig-I (D14G6) Rabbit mAb 3743S from Cell Signaling Technology

Product Specs
ItemRig-I (D14G6) Rabbit mAb
CompanyCell Signaling Technology
Price $336.00 Inquire
Catalog Number3743S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH, M, R, Hm, Mk
TargetRIG-I
HostRabbit
NCBI Gene AliasesDDX58, RIG-I, RIG1, RLR-1, SGMRT2
ClonalityMonoclonal
NCBI Full Gene NameRNA sensor RIG-I
Research AreaImmunology and Inflammation
IsotypeIgG
CloneD14G6
Molecular Weight102

Questions and Answers

Can this antibody be used to study RIG-I signaling in response to viral infections?

Yes, the Rig-I (D14G6) Rabbit mAb has been shown to be effective in studies investigating RIG-I signaling pathways, particularly in the context of viral infections. For instance, it has been utilized to analyze the activation of the mitochondrial antiviral signaling complex upon recognition of viral double-stranded RNA, which is crucial for initiating the immune response ().

What is the recommended dilution for using this antibody in Western blotting?

The recommended dilution for the Rig-I (D14G6) Rabbit mAb in Western blotting is 1:1000, as indicated in various studies that have successfully employed this antibody for protein detection (, ).

Is this antibody suitable for use in immunoprecipitation assays?

Yes, the Rig-I (D14G6) Rabbit mAb is suitable for immunoprecipitation assays. It has been referenced in the literature for its application in isolating RIG-I protein complexes, which is essential for studying the interactions and signaling pathways involved in innate immunity ().

What species does this antibody react with?

The Rig-I (D14G6) Rabbit mAb is reactive with multiple species, including human, mouse, rat, hamster, and non-human primates, making it versatile for various experimental models in immunology and inflammation research.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) TRF2 enriched cytoplasmic chromatin drives cGAS-STAT1-mediated inflammation in senescenceiScienceAugust 21, 2026Renuka Kandhaya-Pillai, Hee Yeon Kim, Jakub Belhadj, Julia M. Sidorova, Tamar Tchkonia, James L. Kirkland, Junko OshimaRabbit mAb,Cell Signaling,Cat #6945; RRID:AB_2210340 "Rig-I (D14G6) Rabbit mAb,",Cell Signaling,Cat #3743; RRID:AB_2269233 "Rad51 (D4B10) Rabbit mAb,",Cell Signaling,Cat #8875; RRID: AB_2799967 KU80,Cell Signaling

(2) VRK3 promotes KSHV infection by suppressing the antiviral type I interferon responsePLoS PathogJuly 27, 2026Caroline J. Yu, Blossom Damania(Cell Signaling Technology, Cat.#18799), RIG-I (D14G6) rabbit monoclonal (Cell Signaling Technology, Cat.# 3743), phospho-STAT1 Tyr701 (58D6) rabbit monoclonal (Cell Signaling Technology, Cat.# 9167), STAT1 (D1K9Y)

(3) Fagopyrum acutatum extract attenuates influenza A virus-stimulated inflammation and acute lung injury in vitro and in vivoJournal of Thoracic DiseaseJune 30, 2026Jinbing Liu, Yingxuan Zhou, Hanwei Huang, Conglin Li, Jiayi Ke, Lifu Liu, Yintong Chen, Taotao Gao, Xiaoli Liang, Youhua Xu, et al.sequences. Western blot Cell Signaling Technology provided the primary antibodies against RIG-I (3743), MDA5 (5321), phosphorylated NF-κB P65 (P-P65) (3033), NF-κB P65 (P65) (8242), phosphorylated IkBα

(4) RNA‐Binding Protein RBM25 Targets the mRNA Stability of GTPase Rab22a to Restrict Viral Entry and InfectionAdvanced ScienceJune 16, 2026Yingying Ding, Huiying Chen, Yuyu Jiang, Chunyan Zhao, Jie Bai, Yan Xiang, Zeting Wang, Xixi Wang, Bing Rui, Wanda Tang, et al.Antibody against RBM25 was from Novus Biologicals (NB100‐57505). Antibodies against β‐Actin (4967), RIG‐I (3743), MAVS (3993), VSV‐G (81454S), p‐TBK1 (5483S), TBK1 (3504), IRF3 (4302S), p‐STAT1(9167S), STAT1 (9172S),

(5) LPAR4 mediates resistance to interferon-induced stress in soft tissue sarcomaSci AdvJune 12, 2026Jin-Fen Xiao, Emily Y. Ko, Ashley Smith, Roberta Piras, Annaliese Fowler, Kristin Ishaya, Longqiang Wang, Bin Wang, Adriana Paulucci-Holthauzen, Sharon M. Landers, et al.protein separation. Antibodies used in the study were obtained from Cell Signaling Technology: RIG-I (3743), MDA-5 (5321), β-Actin (4970), PSMB8 (13635), PSMB9 (87667), STAT1(14994), and p-STAT1(9167). dsRNA

(6) MAVS is important for antiviral defense against influenza A virus in a human respiratory epithelium modelPLoS OneJune 3, 2026Maja Hemberg, Anne Louise Hansen, Jacob Storgaard, Julia Blay-Cadanet, Alice Pedersen, Anne Laugaard Thielke, Christian Kanstrup Holmfollowing antibody solutions diluted in PBS/Tween: anti-RIGI (Cell Signaling, monoclonal, rabbit, #3743, 1:1000), anti-MDA5 (Cell Signaling, #5321, monoclonal, rabbit, 1:1000), anti-MAVS (Cell Signaling,

(7) Cell autonomous inflammation in VEXAS is mediated by cGAS-STINGbioRxivMay 29, 2026Samuel J. Magaziner, Jason C. Collins, Brecca Miller, Patrick Zheng, Amy K. Wang, Jerome Hadjadj, Juan Carlos Baladrán, Maria Sirenko, Maya English, James Bertlin, et al.13647S), phopsho-STING (Cell Signaling, 19781S), cGAS (Cell Signaling, 15102S), RIG-I (Cell Signaling, 3743T), MDA-5 (Cell Signaling, 5321T), IRF-3 (Cell Signaling, 11904T), phospho-IRF3 (Cell Signaling, 4947T),

(8) DHX36 is a regulatory switch in the interferon-mediated antiviral responseSci AdvMay 29, 2026Lisa Weixler, Daniel Hilbig, Philipp S. Simon, Stefan Juranek, Julia Mahlberg, Martin Schlee, Eva Bartok, Markus Hafner, Katrin Paeschkeab76493); anti-TUBA4A (1:5000; Sigma-Aldrich; T5168); anti-RIG-I (1:1000; Cell Signaling Technology; 3743); anti-IFIT1 (1:2000; Cell Signaling Technology; 14769S); anti-phospho-NF-κB p65 (Ser536) (1:500; Cell Signaling Technology

(9) FGF8-mediated TRIM16 regulation promotes K48-linked ubiquitination and degradation of RIG-I to facilitate Influenza a virus immune evasionVirulenceMay 22, 2026Ran Wei, Huixia Zhang, Kaihui Cheng, Song Wang, Zhen Yuan, Sisi Ma, Zhijun YuNP,GeneTex,GTX636199,1:2000 Influenza A virus PB1,GeneTex,GTX125923,1:2000 Influenza A virus PB2,GeneTex,GTX125926,1:2000 Rig-I,CST,3743T,1:1000 TBK1/NAK,CST,3504T,1:1000 Phospho-TBK1/NAK,CST,5483T,1:1000 IRF-3,CST,4302T,1:1000 Phospho-IRF-3,CST,79945T,1:1000 β-Actin,CST,4967S,1:1000 Anti-FGF8

(10) Porcine hemagglutinating encephalomyelitis virus nucleocapsid protein targets RIG-I and IRF3 to evade IFN immunityJournal of VirologyApril 21, 2026Shaoqian Mu, Yuanmao Bai, Ruizhao Qiu, Feilin Zhang, Junchao Shi, Yungang Lan, Feng Gao, Wenqi He, Zi Lihumidity. Antibodies and reagent The following primary antibodies were used: anti-RIG-I antibody (CST, 3743S), anti-MAVs antibody (CST, 4983S), anti-IRF7 antibody (CST, 72073S), anti-IRF3 antibody (CST, 4302S),

(11) STING causes replication stress and nascent DNA degradation via SAMHD1bioRxivMarch 31, 2026Barbara Teodoro-Castro, Rafael Cancado Cancado de Faria, Elena V. Shashkova, Atika Malique, Madison B. Adolph, Lilian N.D. Silva, Susana Gonzalo(1:1000-Cell Signaling-13647), S366p-STING (1:1000-Cell Signaling-50907), RIG-I (1:1000-Cell Signaling-3743S), S33-p-RPA (1:1000 Bethyl-PLA0070), SAMHD1 (1:1000-Cell Singaling-49158), λH2AX (1:1000-Cell Signaling-2577).

(12) RIG-I Stimulation Enhances the Effector Function and Proliferation of Primary Human CD8+ T CellsInternational Journal of Molecular SciencesMarch 27, 2026Adham Abuelola Mohamed, Christina Wallerath, Charlotte Hunkler, Gunther Hartmann, Sanda Stankovic, Andrew G. Brooks, Martin Schleeanti-phospho-TBK1 rabbit mAb (Cell Signaling, #5483), anti-RIG-I rabbit mAb (Cell Signaling, Danvers, MA, USA, #3743), anti-STAT2 rabbit mAb (Cell Signaling, Danvers, MA, USA, #72604) and anti-phospho-STAT2 rabbit mAb

(13) Human RIG-I Antiviral Deficiency Caused by a Dominant-Negative Variant Locked in a Signaling-Inactive StatemedRxivMarch 6, 2026Mihai Solotchi, Huie Jing, Emma Gebauer, Scott J Novick, Bruce D Pascal, Wesley Tung, Pranita Hanpude, Talia Madura, Yu Zhang, Camille Alba, et al.blocked with 5% nonfat milk in 1X PBST and immunoblotted with 1:500 anti-RIG-I (CST D14G6 Rabbit mAb #3743S) and 1:3000 anti-actin (CST 8H10D10 Mouse mAb #3700S) antibodies at 4°C overnight. After washing, the

(14) WNT inhibition activates interferon stimulated gene expression by alleviating epigenetic repression of endogenous retrovirusesScientific ReportsMarch 5, 2026Courtney M. Williams, Jessica Harper Calderon, Varenka Rodriguez DiBlasi, Louis Jinrui Liu, Samer Nuwayhid, Hannah Cevasco, Wei Wang, Rekha Soni, Christina Adler, David D’Ambrosio, et al.GBP2 (53311), phospho-TBK1/NAK (5483), TBK1/NAK (3504), phospho-IRF-3 (37829), MDA-5 (5321), RIG-I (3743), P-STING (19781),STING (13647), LSD1 (2184), DNMT1 (5032), EZH2 (5246), ERK (4695), phospho-ERK (4370

(15) METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune EvasionCancer ResMarch 5, 2026Xiaowei She, Jingqin Lan, Haokun Zhang, Gen Lu, Jialu Xu, Jiakun Zhang, Wenli Zhan, Shengjie Feng, Zejun Rao, Chengxin Yu, et al.Cell Signaling Technology; RRID:AB_2798396), N6-methyladenosine (m6A; ab284130; Abcam; RRID:AB_3717333), RIG-I (3743; Cell Signaling Technology; RRID:AB_2269233), STING (13647; Cell Signaling Technology; RRID:AB_2732796),

(16) Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8Nucleic Acids ResMarch 5, 2026Ana Gázquez-Gutiérrez, Priscilla Chin, Guillermo Peris, Katrina Gordon, Pilar G Marchante, Jeroen Witteveldt, Pablo Tristán-Ramos, Jerome O Rouvière, Lisanne I Knol, Alasdair Ivens, et al.anti-pEIF2α (1:1000, 3398, Cell Signalling), anti-IFIT3 (1:500, ab76818, Abcam), anti-RIG-I (1:1000, 3743, Cell Signalling), anti-DHX9 (1:1000, 70 998 Cell Signalling), anti-ZCCHC8 (1:1000, A301-806 Thermo Fisher Scientific

(17) RIG‐I Mediated Neuron‐Specific IFN Type 1 Signaling in FUS‐ALS Induces Neurodegeneration and Offers New Biomarker‐Driven Individualized Treatment Options for (FUS‐)ALSAdvanced ScienceMarch 1, 2026Marcel Naumann, Theresa M. Wierschin, Stefanie Kretschmer, Banaja P. Dash, Aaron Held, Andrea Salzinger, Kevin Peikert, Anže Karlek, Hannes Glaß, Dajana Großmann, et al.Signaling, D74E4); rabbit anti‐TBK1 (Invitrogen, PA5‐17478), rabbit anti‐RIG‐I (D14G6,Cell Signaling, #3743), rabbit anti‐phospho‐TBK1 (Ser172, D52C2, Cell Signaling, #5483), mouse anti‐IRF3 (Invitrogen, 14‐9947‐82

(18) The human antibacterial factor APOL3 couples lysosomal damage to mitochondrial DNA efflux and type I IFN inductionMol CellFebruary 24, 2026Dominic A. Ritacco, Hamna Shahnawaz, Antonia Oduguwa, Jacob Hawk, Brianna Vizcaino, Donna L. Farber, Ryan G. Gaudetanti-Phospho-STING (Ser366),Cell Signaling,19781 Rabbit anti-TFAM,Cell Signaling,7495 Rabbit anti-RIG-I,Cell Signaling,3743 Rabbit anti-MDA-5,Cell Signaling,5321 anti-HA.11 epitope tag,Biolegend,16B12 Human anti-cardiolipin,USBiological,C1375 Rabbit

(19) RNF20 dynamically regulates RIG-I and MDA5 transcription and degradation via nucleocytoplasmic translocation to balance antiviral signalingPLOS PathogensFebruary 19, 2026Jie Wang, Qiuju Liu, Shurui Zhao, Qi Shao, Feiyu Fu, Kehui Zhang, Jingjiao Ma, Zhaofei Wang, Yaxian Yan, Hengan Wang, et al.21625–1-AP), anti-RNF20 (Santa Cruz biotechnology, sc-517358), anti-RIG-I (Cell Signaling Technologies, 3743), anti-MDA5 (Cell Signaling Technologies, 5321), anti-MAVS (Cell Signaling Technologies, 3993), anti-TBK1

(20) N6-methyladenosine within transmissible gastroenteritis virus genomic RNA inhibits its replication via efficient recognition by RNA sensor RIG-IJournal of VirologyFebruary 17, 2026Jianing Chen, Shengyu Lin, Qianzi Liu, Mengling Gao, Zemei Wang, Jiao Tang, Yaru Cui, Chen Tan, Guangliang Liuanti-YTHDF2 mAb (ab220163, Abcam), rabbit anti-YTHDF3 mAb (ab220161, Abcam), rabbit anti-RIG-I mAb (3743, CST), rabbit anti-IRF3 mAb (4302, CST), rabbit anti-pIRF3 mAb (29047, CST), rabbit anti-MDA5 mAb (5321,

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7/27/2015Review for Rabbit Anti-RIG-I Antibody Liyong Z.Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with PMSF, protease inhibitor mixture, sodium orthovanadate, or ... Read Review

Rig-I (D14G6) Rabbit mAb

Rig-I (D14G6) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Rig-I (D14G6) Rabbit mAb from Cell Signaling Technology

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