Western blot analysis: The extracts of mouse kidney (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).