GATA-1 (D52H6) XP ® Rabbit mAb 3535S from Cell Signaling Technology

Product Specs
ItemGATA-1 (D52H6) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number3535S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC, FC-FP
ReactivityH, M, R
TargetGATA1
HostRabbit
NCBI Gene AliasesERYF1, GATA-1, GF-1, GF1, HAEADA, NF-E1, NFE1, XLANP, XLTDA, XLTT
ClonalityMonoclonal
NCBI Full Gene NameGATA binding protein 1
Research AreaDevelopmental Biology
IsotypeIgG
CloneD52H6
Molecular Weight43

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) TKI-mediated inhibition of NLRP1 inflammasome restores erythropoiesis in DBA syndromeEMBO Molecular MedicineJanuary 9, 2026Juan M Lozano-Gil, Lola Rodríguez-Ruiz, Manuel Palacios, Jorge Peral, Susana Navarro, José L Fuster, Cristina Beléndez, Andrés Jérez, Laura Murillo-Sanjuán, Cristina Díaz-de-Heredia, et al.BSA as standard (Rodriguez-Ruiz et al, 2023). The primary antibodies used were human GATA1 (1:1000, #3535, Cell Signaling), human NLRP1 (1:1000 #AF6788, R&D Systems), human ZAKα (1:1000, #A301-993A, Bethyl Laboratories

(2) Selective AMPKβ1 activation induces fetal hemoglobin in human erythroid cells and sickle cell mice via the noncanonical NRF2 pathwayScience AdvancesNovember 21, 2025Yannis Hara, Viktor T. Lemgart, Samuel Lessard, Courtney J. Mercadante, Jean-Antoine Ribeil, Alexandra Hicks, Pauline Rimmele, David R. Light(Cell Signaling Technology, #50565), anti-ALAS2 (Abcam, ab184964), anti–GATA-1 (Cell Signaling Technology, #3535), anti-AMPKα (Cell Signaling Technology, #5831), anti–phospho-AMPKα-Thr172 (Cell Signaling Technology,

(3) Mitochondrial translation regulates terminal erythroid differentiation by maintaining iron homeostasisSci AdvFebruary 21, 2025Tatsuya Morishima, Md. Fakruddin, Yohei Kanamori, Takeshi Masuda, Akiko Ogawa, Yuxin Wang, Vivien A. C. Schoonenberg, Falk Butter, Yuichiro Arima, Takaaki Akaike, et al.(Thermo Fisher Scientific). The following antibodies were used: a rabbit monoclonal antibody to GATA-1 (#3535, Cell Signaling Technology, Danvers, MA), a rabbit monoclonal antibody to β-actin (#4970, Cell Signaling Technology

(4) GATA1-mediated macrophage polarization via TrkB/cGMP-PKG signaling pathway to promote the development of preeclampsiaEur J Med ResJanuary 7, 2025Wushan Li, Fei Hou, Di Cheng, Fengchun Gao, Jin Wang, Baoxia Cuibought from Abcam, UK; GAPDH (14C10, 1:1000) Rabbit mAb #2118、GATA-1 (D52H6, 1:1000) XP® Rabbit mAb #3535 were bought from Cell Signaling Technology, USA. Dual-luciferase assay The dual-luciferase experiment

(5) Genistin: A Novel Estrogen Analogue Targeting ERβ to Alleviate ThrombocytopeniaInternational Journal of Biological SciencesMarch 25, 2024Xiaoqin Tang, Rui Liao, Ling Zhou, Taian Yi, Mei Ran, Jiesi Luo, Feihong Huang, Anguo Wu, Qibing Mei, Long Wang, et al.(Abmart, TA3241), PI3K (Abmart, TA5112), p-AKT (Abmart, TA0016S), AKT (Abmart, TU421951S), GATA1 (CST, 3535S), NF-E2 (Proteintech, USA, 11089-1-AP), RUNX1 (Proteintech, USA, 25315-1-AP), EGR1 (Proteintech, USA,

(6) ZAKα/P38 kinase signaling pathway regulates hematopoiesis by activating the NLRP1 inflammasomeEMBO molecular medicineOctober 11, 2023Rodríguez-Ruiz L, Lozano-Gil JM, Naranjo-Sánchez E, Martínez-Balsalobre E, Martínez-López A, Lachaud C, Blanquer M, Phung TK, García-Moreno D, Cayuela ML, et al.nitrocellulose membranes (BioRad) for 60 min at 100 V. The primary antibodies used were rabbit human GATA1 (#3535, Cell Signaling), human NLRP1 (#AF6788, R&D Systems and #67980, Biolegend), human LRRFIP1 (#A303‐079A,

(7) PTH regulates osteogenesis and suppresses adipogenesis through Zfp467 in a feed-forward, PTH1R-cyclic AMP-dependent mannereLifeApril 26, 2023Liu H, Wada A, Le I, Le PT, Lee AWF, Zhou J, Gori F, Baron R, Rosen CJ.Antibody,Supplier,Cat Num ACTIN,Santa Cruz Bio,SC47778 PTH1R,Sigma,SAB4502493 GATA1,Cell Signaling Technology,3535T NFκB1 (IP),Cell Signaling Technology,13586S NFκB1 (IB),Cell Signaling Technology,13681S Histone H3,Cell Signaling Technology

(8) Heme-dependent induction of mitophagy program during differentiation of murine erythroid cellsExperimental HematologyFebruary 1, 2023Masatoshi Ikeda, Hiroki Kato, Hiroki Shima, Mitsuyo Matsumoto, Eijiro Furukawa, Yan Yan, Ruiqi Liao, Jian Xu, Akihiko Muto, Tohru Fujiwara, et al.(TreeStar). Immunoblot analysis Immunoblot analysis was performed as described previously 18. Anti-GATA1 antibody (3535s; Cell Signaling Technology) and anti-β-actin antibody (ab3280; Abcam) were used. Heme quantification Cultured

(9) The N(6)-methyladenosine methyltransferase METTL16 enables erythropoiesis through safeguarding genome integrityNature communicationsOctober 28, 2022Yoshinaga M, Han K, Morgens DW, Horii T, Kobayashi R, Tsuruyama T, Hia F, Yasukura S, Kajiya A, Cai T, et al.anti-cleaved caspase-3 (Cell Signaling Technology, #9664, 1:2000), anti-GATA-1 (Cell Signaling Technology, #3535, 1:500), anti-FASN (Santa Cruz Biotechnology, #sc-55580, 1:1000), anti-XPO5 (Santa Cruz Biotechnology,

(10) Caulis Polygoni Multiflori Accelerates Megakaryopoiesis and Thrombopoiesis via Activating PI3K/Akt and MEK/ERK Signaling PathwaysPharmaceuticals (Basel, Switzerland)September 28, 2022Yang X, Wang L, Zeng J, Wu A, Qin M, Wen M, Zhang T, Chen W, Mei Q, Qin D, et al.TA0016S), AKT (Abmart, TU421951S), TAL1 (Proteintech, 55317-1-AP), EGR1 (Abmart, T57177), GATA1 (CST, 3535S), PBX1 (Proteintech, 18204-1-AP). 4.20. Statistical Analysis All the results in this study were presented

(11) Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndromeScientific reportsAugust 26, 2022Ochi T, Fujiwara T, Ono K, Suzuki C, Nikaido M, Inoue D, Kato H, Onodera K, Ichikawa S, Fukuhara N, et al.MA, USA), RNH1 (10345-1-AP, Proteintech, USA), FLAG (#14793, Cell Signaling Technology) and GATA-1 (#3535, Cell Signaling Technology). Statistical analysis All statistical analyses were performed using GraphPad

(12) Downregulation of SATB1 by miRNAs reduces megakaryocyte/erythroid progenitor expansion in preclinical models of Diamond-Blackfan anemiaExperimental hematologyJuly 1, 2022Wilkes MC, Scanlon V, Shibuya A, Cepika AM, Eskin A, Chen Z, Narla A, Glader B, Roncarolo MG, Nelson SF, et al.Cambridge, UK), SATB1 (Abcam No. 49061, RRID: AB_882454,1:500 dilution), GATA1 (Cell Signaling No. 3535, RRID: AB_2108288, 1:500 dilution) HSP70 (Cell Signaling No. 4873, RRID: AB_2119694, 1:1000 dilution),

(13) Targeting GATA1 and p2x7r Locus Binding in Spinal Astrocytes Suppresses Chronic Visceral Pain by Promoting DNA DemethylationNeuroscience bulletinApril 1, 2022Wu YY, Zhang HL, Lu X, Du H, Li YC, Zhang PA, Xu GY.spinal cord were incubated with the primary antibody against GATA1 (1:200, Cell Signaling Technology, 3535), P2X7R (1:100, Alomone labs, APR004), TET2 (1:200, Merck Millipore, ABE364), or TET3 (1:50, Santa Cruz,

(14) GATA binding protein 1 recruits histone deacetylase 2 to the promoter region of nuclear receptor binding protein 2 to affect the tumor microenvironment and malignancy of thyroid carcinomaBioengineeredApril 1, 2022Li M, Jiang H, Chen S, Ma Y.ultrasonicated and centrifuged. The supernatant was diluted in IP dilution buffer. Anti-GATA1 (1:50, #3535, CST), anti-H3K9ac (1:100, GTX128944, Genetex) and IgG (1:1,000, Cat#2729S, CST) were used for IP reaction.

(15) Zkscan3 affects erythroblast development by regulating the transcriptional activity of GATA1 and KLF1 in miceJournal of molecular histologyApril 1, 2022Li Z, Sheng B, Zhang T, Wang T, Chen D, An G, Wang X, Meng H, Yang L.Biolegend), FITC anti-Ter119 (cat: 11-5921-82, eBioscience), anti-GATA-1 (D52H6) XP Rabbit mAb (cat:3535, Cell Signaling Technology), anti-GAPDH (cat:100118,Gene Tex, Irvine, CA, USA), anti-rabbit lgG (cat:HAF008,

(16) Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitmentCell Rep MethodsMarch 28, 2022Baskar R, Chen AF, Favaro P, Reynolds W, Mueller F, Borges L, Jiang S, Park HS, Kool ET, Greenleaf WJ, et al.RRID:AB_314346" CD11c-159Tb,Biolegend,"Cat# 301602, RRID:AB_314172" GATA-1-160Gd,Cell Signalling,"Cat# 3535, RRID:AB_2108288" CD7-162Dy,BD Biosciences,"Cat# 555359, RRID:AB_395762" CD49f-164Dy,Biolegend,"Cat#

(17) Elemental characterization of crystalline silicotitanate following Hanford tank waste processingSeparation Science and TechnologyMay 24, 2021Emily L. Campbell, Amy M. Westesen, Ferdinan C. Colon, Daria Boglaienko, Tatiana G. Levitskaia, Reid A. Petersonspectroscopic measurements and energy band calculations. Journal of Physics: Condensed Matter 11(17), 3535-3545. Walker, D. D. (2004). Digestion of Crystalline Silicotitanate (CST). WSRC-TR-200400526, Rev. 0,

(18) Single nuclei RNA-seq of mouse placental labyrinth developmenteLifeNovember 3, 2020Marsh B, Blelloch R.antibody,R and D Systems,AF1280,IF (1:100) Antibody,Anti-Gata1 Monoclonal antibody,Cell Signaling,3535,IF (1:100) Antibody,Anti-Hbegf Polyclonal antibody,R and D Systems,AF8239,IF (1:20) "Software, algorithm",R,https://www.r-project.org/,, "Software,

(19) FAM122A Inhibits Erythroid Differentiation through GATA1Stem cell reportsSeptember 8, 2020Chen J, Zhou Q, Liu MH, Yang YS, Wang YQ, Huang Y, Chen GQ.specific primary antibodies overnight at 4°C. The following primary antibodies were used: anti-GATA1 (3535, Cell Signaling Technology), anti-GATA2 (ab70366, Abcam), anti-hemoglobin α (14537-1-AP, Proteintech),

(20) Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinaseNature communicationsJuly 3, 2020Wilkes MC, Siva K, Chen J, Varetti G, Youn MY, Chae H, Ek F, Olsson R, Lundbäck T, Dever DP, et al.dilution, #PAS-21877; Invitrogen; 1:1000, #PAS-25953; Invitrogen; 1:1000, #94350; Cell Signaling; 1:1000, #3535; Cell Signaling; 1:500), phosphoThr298-NLK (orb157946; biorbyt; 1:350 dilution), c-Myb (#12319; Cell Signaling

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GATA-1 (D52H6) XP ® Rabbit mAb

GATA-1 (D52H6) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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GATA-1 (D52H6) XP ® Rabbit mAb from Cell Signaling Technology

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