Neuropilin-1 (D62C6) Rabbit mAb 3725S from Cell Signaling Technology

Product Specs
ItemNeuropilin-1 (D62C6) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3725S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R
TargetNeuropilin1
HostRabbit
NCBI Gene AliasesBDCA4, CD304, NP1, NRP, VEGF165R
ClonalityMonoclonal
NCBI Full Gene Nameneuropilin 1
Research AreaNeuroscience
IsotypeIgG
CloneD62C6
Molecular Weight120

Questions and Answers

Can this antibody be used to study the role of Neuropilin-1 in angiogenesis?

Yes, the Neuropilin-1 (D62C6) Rabbit mAb has been utilized in studies investigating its role in angiogenesis. For instance, it was shown that Neuropilin-1 is involved in endothelial cell migration and focal adhesion remodeling during sprouting angiogenesis, as detailed in the study by Cao et al. (2017) .

Is this antibody effective for Western blot analysis in different species?

The Neuropilin-1 (D62C6) Rabbit mAb has been demonstrated to work effectively in Western blot analysis for multiple species, including human, mouse, and rat, as indicated by its reactivity profile.

How has this antibody been applied in research related to cancer?

This antibody has been cited in research examining the role of Neuropilin-1 in cancer metastasis. For example, Huang et al. (2019) reported that Neuropilin-1 splice variants promote colorectal cancer metastasis by activating endosomal signals, highlighting the importance of this receptor in tumor biology .

What experimental techniques can this antibody be used with?

The Neuropilin-1 (D62C6) Rabbit mAb is suitable for use in Western blotting and immunoprecipitation, as evidenced by its application in various studies, including those investigating the signaling pathways involved in neuronal morphogenesis and cancer progression.

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Vascular Endothelial Growth Factor B Modulates Cardiac Functions via Ferroptosis Pathways in Post-Myocardial InfarctionCellsOctober 21, 2025Sai Manasa Varanasi, Ankit Sabharwal, Shreyartha Mukherjee, Huzaifa Muhammad, Riya Kar, Carter Magnano, Anya Dorairaj, Enfeng Wang, Shamit Dutta, Pritam Das, et al.(CST #9272S), pAKT (CST #9271S), BAX (CST #2772S), BCL2 (CST #15071S), GPX4 (CST #52455S), NRP-1 (CST #3725S), cleaved caspase-3 (CST #9661S), caspase-3 (CST #9662), PGC1α (CST #2178S), β-actin (Sigma)) was carried

(2) Glycosaminoglycan modification of NRP1 exon 4-skipping variant drives colorectal cancer metastasis via endosomal-exosomal traffickingCancer LettersJune 28, 2025Yiwei Gu, Qing Ye, Xiuping Huang, Yanan Cao, Luksana Chaiswing, Qing-Bai Sheformvar carbon-coated nickel grids, blocked, and incubated with rabbit anti-NRP1 monoclonal antibody (#3725, Cell Signaling Technology), followed by incubation with an anti-rabbit secondary antibody conjugated

(3) Modeling acquired TKI resistance and effective combination therapeutic strategies in murine RET+ lung adenocarcinomabioRxivJune 7, 2025Trista K Hinz, Anh T Le, Tristan Doan, Abby Ast, Sophia Jaramillo, Samantha Haines, Andre Navarro, Tejas Patil, Raphael A Nemenoff, Lynn E HeasleyCell Signaling Technology (Danvers, MA): RET-pY905 (cs-3221), MET-pY1234/1235 (sc-3129), total MET (cs-3127), NRP-1 (cs-3725), pT202/pY204-ERK (cs-9101), pS473-AKT (cs-4060), total AKT (cs-9272), pY542-PTPN11 (cs-3751), pY580-PTPN11

(4) Repeated social defeat in male mice induced unique RNA profiles in projection neurons from the amygdala to the hippocampusBrain, Behavior, & Immunity - HealthFebruary 1, 2025Rebecca G. Biltz, Wenyuan Yin, Ethan J. Goodman, Lynde M. Wangler, Amara C. Davis, Braedan T. Oliver, Jonathan P. Godbout, John F. SheridanSynaptic Systems, #195004, Synaptic Systems) and rabbit anti-mouse HA (1:500; Cell Signaling, cat# 3725 S). Sections were incubated overnight at 4 °C. Next, sections were washed in PBS and incubated with

(5) NRP1 knockdown inhibits the invasion and migration of rhabdoid tumor of the kidney cellsPediatric Surgery InternationalAugust 12, 2024Bin Yamaoka, Eri Nagasaki-Maeoka, Shota Uekusa, Eri Muto-Fujita, Naoko Abe, Kyoko Fujiwara, Tsugumichi Koshinaga, Shuichiro Uehararabbit monoclonal antibodies (Cell Signaling Technology, Beverly, MA, USA) for NRP1 (catalog no. D62C6; 3725S; dilution, 1:500) or β-actin (catalog no. 13E5; 4970S; dilution, 1:1,000) at 4 °C. After 24 h of incubation,

(6) Conditional, tissue-specific CRISPR/Cas9 vector system in zebrafish reveals the role of neuropilin-1b in heart regenerationArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2023Ramcharan Singh Singh Angom, Ying Wang, Enfeng Wang, Shamit Kumar Dutta, Debabrata Mukhopadhyaypurchased from Cell Signaling (Danvers, MA). BrdU (Bu20a) Mouse mAb #5292, Neuropilin-1 (D62C6) Rabbit mAb #3725, VE-Cadherin (D87F2) XP® Rabbit mAb #2500. Anti-MYH6 antibody was purchased from Abcam (Anti-MYH6 antibody [3–48 G5C7

(7) Beta IV spectrin inhibits the metastatic growth of melanoma by suppressing VEGFR2‐driven tumor angiogenesisCancer MedicineSeptember 7, 2023Eun‐A. Kwak, Tasmia Ahmed, Paola Cruz Flores, Hannah R. Ortiz, Paul R. Langlais, Karthikeyan Mythreye, Nam Y. LeeCell Signaling Technology [(VEGFR2‐ #9698, p‐AKT S473‐ #4060, p‐p44/42‐ #9101, p‐p38‐ #3871, p‐PLCγ2‐#4511, neuropilin 1‐ #3725)]. Antibodies to βIV‐spectrin and β‐actin were purchased from Santa Cruz Biotechnology (#SC514744) and

(8) Conditional, tissue-specific CRISPR/Cas9 vector system in zebrafish reveals the role of neuropilin-1b in heart regenerationArterioscler Thromb Vasc BiolAugust 31, 2023Ramcharan Singh Angom, Ying Wang, Enfeng Wang, Shamit Dutta, Debabrata Mukhopadhyaypurchased from Cell Signaling (Danvers, MA). BrdU (Bu20a) Mouse mAb #5292, Neuropilin-1 (D62C6) Rabbit mAb #3725, VE-Cadherin (D87F2) XP® Rabbit mAb #2500. Anti-MYH6 antibody was purchased from Abcam (Anti-MYH6 antibody [3–48 G5C7

(9) Piezo1 as a force-through-membrane sensor in red blood cellseLifeDecember 14, 2022Vaisey G, Banerjee P, North AJ, Haselwandter CA, MacKinnon R.(MatTeK) and blocked in blocking buffer before incubation with 1:500 dilution of rabbit anti-HA primary (3725; Cell Signaling Technology) for 1 hr at room temperature. Cells were then washed in blocking buffer

(10) Endothelial VEGFR Coreceptors Neuropilin-1 and Neuropilin-2 Are Essential for Tumor AngiogenesisCancer Res CommunDecember 1, 2022Benwell CJ, Johnson RT, Taylor JAGE, Price CA, Robinson SD.antibodies: p-VEGFR-2Y1175 (2478, CST; RRID:AB_331377), VEGFR-2 (2479, CST; RRID:AB_2212507), NRP1 (3725, CST; RRID:AB_2155231), NRP2 (3326, CST), HSC70 (Sc-7298, SCB; RRID:AB_627761), EDA-FN (F6140, Sigma;

(11) Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual systemNature communicationsOctober 13, 2022Vilallongue N, Schaeffer J, Hesse AM, Delpech C, Blot B, Paccard A, Plissonnier E, Excoffier B, Couté Y, Belin S, et al.anti-Sema7A (1:1000, Rabbit, Abcam, ab23578), anti-Neuropilin-1 (1:1000, Rabbit, Cell signalling Technology, #3725), anti-Neuropilin-2 (1:1000, Rabbit, Cell signalling Technology, #3366), anti-Plexin-A4 (1:1000, Rabbit,

(12) Differential dependency of human glioblastoma cells on vascular endothelial growth factor‑A signaling via neuropilin‑1International journal of oncologyOctober 1, 2022Lee J, Chong K, Lee J, Kim C, Kim JH, Choi K, Choi C.no. 8556), FAK (cat. no. 71433), p-AKT (Ser473; cat. no. 4060), AKT (cat. no. 9272), NRP-1 (cat. no. 3725), caspase-3 (cat. no. 9662), cleaved caspase-3 (cat. no. 9664), and glyceraldehyde 3-phosphate dehydrogenase

(13) Syndecan-2 selectively regulates VEGF-induced vascular permeabilityNat Cardiovasc ResMay 1, 2022Corti F, Ristori E, Rivera-Molina F, Toomre D, Zhang J, Mihailovic J, Zhuang ZW, Simons M.Münster, Germany), DEP1 (WB 1:200, Santa Cruz #sc-21761 (143–41)) NRP1 (WB 1:1000, Cell signaling #3725,), HA-tag (WB 1:1000, Cell signaling #3724), rabbit anti-Rab5 (Cell Signaling, #3547, IHC 1:200); goat

(14) Inactivation of EGLN3 hydroxylase facilitates Erk3 degradation via autophagy and impedes lung cancer growthOncogeneMarch 1, 2022Jin Y, Pan Y, Zheng S, Liu Y, Xu J, Peng Y, Zhang Z, Wang Y, Xiong Y, Xu L, et al.Santa Cruz Biotechnology, sc-40), anti-GST (Yeasen, 30902ES60), anti-NRP1 (Cell Signaling Technology, #3725), anti-NRP2 (Cell Signaling Technology, #3366), anti-EGLN3 (Novus, NB100–303), anti-Erk3 (Santa Cruz Biotechnology

(15) Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mousePhysiological reportsSeptember 1, 2021Tamura Y, Jee E, Kouzaki K, Kotani T, Nakazato K.NBP3‐00492, Novus Biologicals); FURIN (1:2000, 18413‐1‐AP, Proteintech); and Neuropilin‐1 (1:2000, 3725, Cell Signaling Technology). 2.7 Statistical analysis Data were expressed as mean ± SE for individual

(16) Interactions between semaphorins and plexin–neuropilin receptor complexes in the membranes of live cellsJournal of Biological ChemistryAugust 1, 2021Shaun M. Christie, Jing Hao, Erin Tracy, Matthias Buck, Jennifer S. Yu, Adam W. Smithconfirm the expression of the samples. Primary antibodies used are Nrp1 (Cell Signaling; catalog no. 3725), plexin A2 (R&D; catalog no. MAB5486), plexin D1 (R&D; catalog no. AF4160), and plexin A4 (R&D; catalog

(17) Activation of the STAT5 Signaling Pathway by Yiqi Jiedu Formula Induces Regulatory T Cell-Mediated Alleviation of Corneal Immunopathological Damage in Mice With Recurrent Herpes Simplex KeratitisFrontiers in pharmacologyJanuary 1, 2021Xiao S, Yang Y, Miao W, Lyu C, Tao J, Yu Y.antibodies (anti-p-STAT5, 1:2000, CST, 4322; anti-STAT5, 1:2000, CST, 94205; anti-Neuropilin-1, 1:1500, CST, 3725; and anti-β-actin, 1:2000, CST, 4970). Next, the PVDF membranes were washed with TBST three times and

(18) Modular and Distinct Plexin-A4/FARP2/Rac1 Signaling Controls Dendrite MorphogenesisThe Journal of neuroscience : the official journal of the Society for NeuroscienceJuly 8, 2020Danelon V, Goldner R, Martinez E, Gokhman I, Wang K, Yaron A, Tran TS.#3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6) anti-mouse (Cell Signaling Technology, catalog #3725), and 1:30,000 mouse anti-actin antibody C4 (MP Biomedicals, catalog #08691001). Antibodies were diluted

(19) CircHIPK3 Promotes Metastasis of Gastric Cancer via miR-653-5p/miR-338-3p-NRP1 Axis Under a Long-Term Hypoxic MicroenvironmentFrontiers in oncologyJanuary 1, 2020Jin Y, Che X, Qu X, Li X, Lu W, Wu J, Wang Y, Hou K, Li C, Zhang X, et al.37°C. Reagents and Antibodies AKT (#9272), phosphorylated (p)-AKT (#9271), p-ERK (#4370), and NRP1 (#3725) antibodies were obtained from Cell Signaling Technology (Danvers, United States). β-actin (sc-47778)

(20) MicroRNA-148a/b-3p regulates angiogenesis by targeting neuropilin-1 in endothelial cellsExperimental & molecular medicineNovember 13, 2019Kim H, Ko Y, Park H, Zhang H, Jeong Y, Kim Y, Noh M, Park S, Kim YM, Kwon YG.antibodies were obtained from Cell Signaling Technology and were used at a 1:1000 dilution: NRP1 (Cat. No. 3725), phospho-VEGFR2 (Cat. No. 2478), VEGFR2 (Cat. No. 3479), phospho-FAK (Cat. No. 2541), FAK (Cat. No.

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Neuropilin-1 (D62C6) Rabbit mAb

Neuropilin-1 (D62C6) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Neuropilin-1 (D62C6) Rabbit mAb from Cell Signaling Technology

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