Rabbit (DA1E) mAb IgG XP ® Isotype Control 3900S from Cell Signaling Technology

Product Specs
ItemRabbit (DA1E) mAb IgG XP ® Isotype Control
CompanyCell Signaling Technology
Price $177.00 Inquire
Catalog Number3900S
Quantity250 µg
ApplicationsIP, IHC-P, IF-IC, FC-FP, FC-L, ChIP
HostRabbit
IsotypeIgG
CloneDA1E
ClonalityMonoclonal
Research AreaRelated Products

Questions and Answers

Can this isotype control be used in chromatin immunoprecipitation experiments?

Yes, the Rabbit (DA1E) mAb IgG XP® Isotype Control has been utilized in chromatin immunoprecipitation (ChIP) assays, as indicated in various studies, including those that employed it as a non-specific IgG control to assess specific antibody binding.

What applications has this isotype control been shown to work in?

The Rabbit (DA1E) mAb IgG XP® Isotype Control is applicable in several experimental techniques, including flow cytometry (FCM), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHC-p), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).

Is there any evidence of this isotype control being referenced in scientific literature?

Yes, the Rabbit (DA1E) mAb IgG XP® Isotype Control has been cited in 129 scientific articles, demonstrating its widespread use and acceptance in various research contexts.

What is the significance of using an isotype control like this one in experiments?

Using an isotype control, such as the Rabbit (DA1E) mAb IgG XP® Isotype Control, is crucial for distinguishing specific antibody binding from non-specific background signal, thereby enhancing the reliability of experimental results in applications like immunofluorescence and immunoprecipitation.

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Website: Technical Support

(1) TIPE2 gene knockdown in mice attenuates experimental colitis by diminishing inflammatory cell infiltrationMol Ther Nucleic AcidsJune 16, 2026Yexiao Tang, Ping Li, Minghui Li, Pengchao Zhang, Zhen Lu, Hang Qiao, Jing Wei, Zhiming Xu, Xiaochun Wan, Shu Xu, et al.at 4°C using either anti-TIPE2 (E5Q2S; CST, 53842S; 1:500) or an isotype control (rabbit IgG; CST, 3900S; 1:500). After washing, cells were aliquoted for surface marker staining for 30 min at 4°C using one

(2) iNOS modulates inflammatory responses in an NO-independent manner through direct interaction with IRG1 in mitochondriaNat MetabApril 10, 2026Marina Diotallevi, Carlos Outeiral, Priyanka Patel, Gareth S. D. Purvis, Surawee Chuaiphichai, Thomas Nicol, Faseeha Ayaz, Daniel A. Nissley, Ganna O. Krasnoselska, Svenja Hester, et al.(anti-IRG1 (ab222411)) and control antibodies (rabbit IgG isotype control; Cell Signaling Technology, 3900S). After washing beads from the cell lysate, the target protein was either eluted in 2.5× Laemmli loading

(3) Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopySTAR ProtocMarch 19, 2026Anna Jaeschke, Matt S. Hepburn, Alireza Mowla, Hagen Eckert, Brendan F. Kennedy, Chii Jou Chan"Rabbit (DA1E) mAb IgG XP® Isotype Control, 1:30",Cell Signalling Technologies (Research Instruments Pte Ltd.),3900S ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(4) ACOD1 regulates microglial arginine metabolism and inflammatory responsesFront ImmunolMarch 16, 2026Eleftheria Karadima, Canelif Yilmaz, Anupam Sinha, Georgia Fodelianaki, Sofia Dimothyra, Nikolaos Nirakis, Sofia Traikov, Nicola Zamboni, Ben Wielockx, Panayotis Verginis, et al.anti-histone H3K9ac (Active motif, 39918) or rabbit (DA1E) mAb IgG XP® isotype control (Cell signaling, 3900s) overnight at 4 °C with orbital mixing. Afterwards, the samples were placed on a magnetic stand to clear

(5) A Dimeric Rocaglate Promotes Multivalent eIF4A-RNA AssemblybioRxivMarch 13, 2026Jie Liu, Megan K. Moore, Kevin Lou, Douglas R. Wassarman, Abolfazl Arab, Samuel Ojeda, Barbara Karakyriakou, Ann-Sophie Koglin, Christopher J. Ott, Luke A. Gilbert, et al.anti-eIF4A2 (Santa Cruz Biotechnology, SC-137148), or IgG control antibodies (Cell Signaling Technology, 3900S or 5415S). Where indicated, RNase A (10 μg/mL; NEB, T3018L) was added during the incubation. Protein

(6) Transcriptional regulation of ventral hippocampus-nucleus accumbens circuit excitability drives cocaine seekingSci AdvMarch 6, 2026Andrew L. Eagle, Chiho Sugimoto, Marie A. Doyle, Daniela Anderson, Seyedeh Leila Mousavi, Megan M. Dykstra, Hayley M. Kuhn, Brooklynn R. Murray, Ryan M. Bastle, Sarah Simmons, et al.polyclonal FosB antibody (lot 3; 2251S, Cell Signaling Technology) or 2 μg of rabbit polyclonal IgG isotype (3900S, Cell Signaling Technology). Antibody-bound chromatin fragments were isolated with protein G plus/protein A agarose

(7) UTX coordinates TCF1 and STAT3 to control progenitor CD8+ T cell fate in autoimmune diabetesJournal of Clinical InvestigationFebruary 16, 2026Ho-Chung Chen, Madison F. Bang, Hsing-Hui Wang, Karl B. Shpargel, Lisa A. Kohn, David Sailer, Shile Zhang, Ethan C. McCarthy, Maryamsadat Seyedsadr, Satchel Stevens, et al.Cell Signaling Rabbit monoclonal antibody no. 33510 or IgG isotype control: Cell Signaling Technology, 3900S) in antibody buffer (20 mM HEPES pH 7.5; 150 mM NaCl, 0.5 mM spermidine, 1× protease inhibitor cocktail

(8) Deficiency of lysosomal TMEM175 in myeloid macrophages exerts anti-tumor immunity via inflammasome and cross-presentation pathwayNature CommunicationsFebruary 14, 2026Ziqi Zhang, Xue li, Tianqi Lu, Jingyun Yang, Xuejiao Han, Zhenfei Bi, Jingwen Luo, Aqu Alu, Weiqi Hong, Siyuan Chen, et al.by RIPA. Protein lysates were incubated with 1 µg/mL rabbit IgG isotype (Cell Signaling Technology, 3900S) and 20 µL Protein A + G Agarose (Beyotime, P2012) at 4 °C for 30 min to avoid non-specific combination.

(9) Alterations in Mitochondrial DNA in Corneal Fibroblast and Myofibroblast Post InjuryInvestigative Ophthalmology & Visual ScienceJanuary 16, 2026Nishant R. Sinha, Alexandria C. Hofmann, Laila A. Suleiman, Maxwell T. Jeffrey, Rajnish Kumar, Rajiv R. MohanThermo Fisher Scientific) for 4 hours at room temperature. Rabbit (DA1E) mAb IgG XP Isotype Control (3900S; Cell Signaling Technology) was used for TFAM and Invitrogen Mouse IgG2a, kappa Isotype Control (14-4724-82;

(10) The radiotherapy induced cyclic GMP-AMP-synthase re-localization and immune signature predicts HNSCC treatment efficacyCommunications MedicineJanuary 16, 2026Vera E. Mekers, Paul N. Span, Maaike Looman, Lune van den Bogaard, Vera Kho, Willem J. G. Melchers, Johan Bussink, Johannes H.A.M. Kaanders, Marleen Ansems, Gosse J. Ademacat. no. 32f6; 1:500) and incubated for 30 min at 37 °C. Isotype control (CST; clone DA1E; cat. no. 3900S) for a-mcGAS and a-hcGAS, or buffer without antibody for a-mNG were taken along as negative control.

(11) A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesisScienceDecember 11, 2025Tuan-Anh Nguyen, Jung-Ming G. Lin, Anne-Sophie M. C. Marques, Maximilian Fottner, Ludwig G. Bauer, Andreas Reicher, Diana Daum, Lorenzo Scrofani, Yusi Liu, Carol Cheng, et al.Cleared lysates were spiked with 1 µg of PPAT (15401-1-AP, Proteintech) or rabbit IgG control antibody (3900S, Cell Signaling Technologies) and incubated o.n. at 4 °C while being rotated. 30 µl magnetic protein

(12) CCR2 improves tumor directed CAR-T cell trafficking in ovarian cancerFront PharmacolDecember 5, 2025Raj Kumar, Irva E. Veillard, Mengyao Xu, Linah Al-Alem, Bo R. Rueda, Oladapo O. Yekudilution instructions. Isotypes Anti-Rabbit (DA1E) mAb IgG XP® Isotype Control (Cell Signaling Technology 3900S) and Anti-Mouse IgG1 control isotype (Dako/Agilent X093101-2) were used as controls according to manufacturer’s

(13) Intrinsic mechanotransduction during apical constriction licenses lineage competence in pluripotent stem cellsbioRxivDecember 3, 2025Mehdi Seif Hamouda, Celine Labouesse, George W. Wylde, Daniel Yamamoto, Yekaterina A. Miroshnikova, Srinjan Basu, Waterman M Waterman, Kevin J. Chalutimmunoprecipitation or equivalent mass of Rabbit IgG isotype control (Rabbit (DA1E) mAb IgG XP® Isotype Control #3900S). This was incubated overnight at 4°C on a spinning rotor. The next day, 40uL of washed Protein A Dynabead

(14) MUCIN 1 confers inflammatory memory of tyrosine kinase inhibitor resistance in non-small cell lung cancerSignal Transduction and Targeted TherapyNovember 28, 2025Shinkichi Takamori, Naoki Haratake, Atrayee Bhattacharya, Chie Kikutake, Hiroki Ozawa, Keisuke Shigeta, Ayako Nakashoji, Hideko Isozaki, Mototsugu Shimokawa, Mikita Suyama, et al.described.39 Chromatin immunoprecipitation Chromatin immunoprecipitation (ChIP) was performed using a control IgG (#3900S, CST), anti–MUC1-C (#16564S, CST), anti-STAT1 (#ab109320, Abcam), anti-PBRM1 (A301–591 A, Bethyl Laboratories),

(15) Targeting the Notch1‐YY1‐ICAM1 Signaling Axis Enhances the Efficacy of Immunotherapy in HCC by Activating CD8 + T‐Cell‐Driven Cancer Cell PyroptosisAdvanced ScienceNovember 21, 2025Ke Zhu, Fa‐Peng Zhang, Chao Qin, Zhi‐Xiao Song, Cai‐Ni Yang, Shu‐Sheng Lin, Xian‐Huan Yu, Wen‐Rui Wu, Chao‐Qun Liu, Chao Liu, et al.(Cell Signaling Technology, Cat. no. 46395S) or IgG control antibody (Cell Signaling Technology, Cat. no. 3900S) as a control. The enriched chromatin was purified to obtain DNA, which was used for RT‒PCR analysis,

(16) Preservation of ALYREF Phase Separation Mitigates Doxorubicin‐Induced Cardiomyocyte DNA Damage and CardiotoxicityAdvanced ScienceNovember 1, 2025Xinlu Gao, Yifu Shen, Zhihui Xiao, Zhenbo Han, Xu Liu, Ao Cai, Yanan Tian, Guang Lian, Wenya Ma, Yining Liu, et al.taken from each group, added with ALYREF antibody (10 µg, AB202894, Abcam, UK) or control IgG (cat. no‐3900s, Cell Signaling) and incubated overnight at 4 °C on a rotary shaker. The “antigen–antibody” mixture

(17) Prolonged skin allograft survival by rM180 amelogenin in a murine skin transplantation modelFrontiers in ImmunologyOctober 27, 2025Miyu Shida, Terukazu Sanui, Karen Yotsumoto, Jinfeng Li, Mwannes Ahmad, Meng Xiao, Ziyu Wang, Chikako Hayashi, Yuki Nishimura, Takanori Shinjo, et al.Mouse (G3A1) mAb IgG1 isotype control (5415S, Cell Signaling Technology) at 1:500 dilution, Rabbit (DA1E) mAb IgG XP isotype control (3900S, Cell Signaling Technology) at 1:500 dilution, Anti-Ym-1 + Ym-2 (Chil4) antibody ([EPR15263]; Abcam)

(18) MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progressionOncogeneOctober 7, 2025Hiroki Ozawa, Yin Wang, Henry G. Withers, Naoki Haratake, Ayako Nakashoji, Atrayee Bhattacharya, Atsushi Fushimi, Chie Kikutake, Kazuhiro Yamanoi, Shaowen White, et al.anti-MUC1-C (HM-1630-P1ABX; Thermo Fisher Scientific, Waltham, MA, USA) or a rabbit isotype control IgG (3900S, Cell Signaling Technology (CST), Danvers, MA, USA) using the Pierce Classic Magnetic Co-IP Kit (Thermo

(19) Catalytic inhibition of KAT6/KAT7 enhances the efficacy and overcomes primary and acquired resistance to Menin inhibitors in MLL leukaemiaCancer DiscoveryOctober 6, 2025Shellaina J.V. Gordon, Florian Perner, Laura MacPherson, Katie A. Fennell, Daniela V. Wenge, Wallace Bourgeois, Tabea Klaus, Thomas Plenge, Anelya Murat, Jelena Petrovic, et al.anti-rabbit MLL1(Bethyl, A300–086A, RRID:AB_242510), anti-rabbit IgG XP Isotype Control D1AE (CST, 3900S). 5 μg of antibody was used for MLL1, Menin and IgG isotype control immunoprecipitations. Cut&Tag: anti-rabbit

(20) Three-dimensional quantitative micro-elastography reveals alterations in spatial elasticity patterns in murine ovaries during ageingCommun BiolOctober 2, 2025Anna Jaeschke, Matt S. Hepburn, Alireza Mowla, Brendan F. Kennedy, Chii J. ChanaSMA,Abcam,ab32575,1:500 Rabbit (DA1E) mAb IgG XP® Isotype Control,Cell Signalling Technologies (Research Instruments Pte Ltd),3900S,1:30 IF staining reagents

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Rabbit (DA1E) mAb IgG XP ® Isotype Control

Rabbit (DA1E) mAb IgG XP ® Isotype Control from Cell Signaling Technology

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Cell Signaling Technology

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Rabbit (DA1E) mAb IgG XP ® Isotype Control from Cell Signaling Technology

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