Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb 3377S from Cell Signaling Technology

Product Specs
ItemPhospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $431.00 Inquire
Catalog Number3377S
ClonalityMonoclonal
NCBI Full Gene NameH3 clustered histone 1
ReactivityHu, Ms, Rt, Mk, Zf
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
CloneD2C8
Molecular Weight17
ApplicationsWB, FCM, ICC, IF
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
HostRabbit
ModificationsH3 (Ser10) phosphate
TargetH3 (Ser10) phosphate
Quantity100 µl

Questions and Answers

What specific applications has the Phospho-Histone H3 (Ser10) antibody been used for in research studies?

The Phospho-Histone H3 (Ser10) antibody has been shown to work in applications such as immunocytochemistry and Western blotting, particularly in studies investigating cell cycle regulation and mitotic progression. For instance, it has been utilized to assess histone H3 phosphorylation levels, which are critical markers for mitotic entry and chromosomal stability during cell division.

How does the Phospho-Histone H3 (Ser10) antibody contribute to understanding cell cycle dynamics?

This antibody is pivotal in studies examining the role of phosphorylation in cell cycle regulation. Research has demonstrated that phosphorylation of histone H3 at Ser10 is associated with chromatin condensation during mitosis, thus providing insights into the mechanisms of mitotic progression and the regulation of chromosomal stability, as highlighted in various studies including those focused on the anaphase-promoting complex/cyclosome.

Can the Phospho-Histone H3 (Ser10) antibody be used to study cancer cell behavior?

Yes, the antibody has been referenced in studies exploring cancer cell behavior, particularly in the context of mitotic checkpoints and chromosomal instability. For example, it has been used to investigate the effects of various treatments on histone modifications in cancer cells, which can reveal insights into therapeutic resistance and cell proliferation dynamics.

Is there any evidence of the Phospho-Histone H3 (Ser10) antibody being used in specific cancer types?

The antibody has been cited in research related to various cancer types, including studies on head and neck squamous cell carcinoma and breast cancer. These studies often focus on the role of histone modifications in tumor progression and response to therapies, indicating its relevance in cancer research.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Glycine promotes cardiomyocyte proliferation and heart regeneration via the GCN2/AKT signaling axisTheranosticsApril 23, 2026Feng Wang, Xia Zhang, Yang Tang, Yanji He, Wei Wang, Andy Zeng, Lijia Liang, Jing Wang, Chunyu Zengtroponin T (CTNT, Invitrogen, MA5-12960, 1:200), Ki67 (CST, 9027, 1:200), phospho-histone H3 (CST, 3377, 1:200), GCN2 (Santa Cruz, sc-374609, 1:200), and AKT (CST, 4691S, 1:200). For co-localization analysis,

(2) Wnt signaling patterns a central-peripheral body axis in the early-diverging metazoan Trichoplax adhaerensMol Biol EvolApril 8, 2026Guotao Di, Minjun Jin, Wanqing Li, Meng Yuan, Yifan Zhang, Chengtian Zhaoovernight at 4 °C with the primary antibody against phospho-histone H3 (pH3) (Cell Signaling Technology, #3377), diluted 1:500 in blocking solution. Afterward, secondary antibodies were applied and incubated overnight

(3) BMAL2 is a druggable target for ovarian clear cell carcinoma (OCCC)EMBO Molecular MedicineApril 3, 2026Grace Y T Tan, Pei-Yi Lin, Li-Tzu Cheng, Yu-Sheng Tsai Yuan, Shih-Han Huang, Chen-Hsin Albert Yu, Chao-Tsen Chen, Peter Chi, Wendy W Hwang-Versluesfor 1 h at room temperature and incubated with primary antibodies against phospho-histone H3 (pHH3; #3377 s, Cell signaling technology; RRID:AB_1549592, 1:800) or γH2AX (#2577 s, Cell signaling technology,

(4) Mosaic gastruloids reveal a temporal restriction for developmental cell competitionNature Cell BiologyApril 1, 2026Joshua D. Frenster, Stephen Babin, Pablo Casani-Galdon, Joel B. Josende-Garcia, Pau Pascual-Mas, Gaëlle Robertson, Shlomit Edri, Alexandra E. Wehmeyer, Sebastian J. Arnold, Jordi Garcia Ojalvo, et al.clone 1C12; Cell Signaling, 2524S), rabbit anti-phospho-histone 3 (1:1,600, clone D2C8, Cell Signaling, 3377), rabbit anti-cleaved caspase 3 (1:5,000, clone 5A1E, Cell Signaling, 9664), rabbit anti-YAP (1:200,

(5) Hyperinnervation inhibits organ-level regeneration in mammalian skinCellMarch 20, 2026Hannah T. Tam, Jingyu Peng, Rebecca Freeman, Yulia Shwartz, Shlomi Brielle, Sakshi Garg, Siti Rahmayanti, Stephen J. Crocker, Devin Coon, Ya-Chieh Hsu(1:1000),Abcam,Cat#ab108986;RRID:AB_10891773 Rabbit anti Phospho-Histone H3 (Ser10) (1:2000),Cell Signaling Technology,Cat#3377S;RRID:AB_1549592 Rabbit anti RFP (pre-adsorbed) (1:1000),Rockland,Cat#600-401-379;RRID:AB_2209751 Rabbit

(6) Spatiotemporal dynamics of Mcl-1 abundance and its influence on apoptosis susceptibilitybioRxivFebruary 12, 2026Fabian Klötzer, Nadine Pollak, Anna Baatz, Batuhan Kisakol, Fiona Ginty, Daniel B. Longley, Jochen H. M. Prehn, Markus Rehmflow cytometry (FC)), mouse anti-RFP (ChromoTek 6g6, 1:1000 WB), rabbit anti-Phospho-Histone H3 (D2C2, Cell Signaling #3377 and #8552, 1:50 FC), rabbit anti-Tom20 (Cell Signaling #42406, 1:1000 WB). Secondary antibodies were

(7) Molecular basis for the activation of Aurora A and Plk1 kinases during mitotic entryThe EMBO JournalJanuary 28, 2026Anaïs Pillan, Philippine Ormancey, Celia Ben Choug, Stephen Orlicky, Nicolas Tavernier, Lucie Van Hove, Batool Ossareh-Nazari, Nicolas Joly, Frank Sicheri, Thierry Lorca, et al.Plk1,Bethyl,Cat#A300-250A Rabbit anti-GST,Cell Signaling,#2622 Rabbit anti-Phospho-Histone H3 (Ser10),Cell Signaling,Cat#3377S Rabbit anti-Histone H3,Abcam,Cat#AB1791 Mouse anti-MBP,NEB biolabs,Cat#E8032S Rabbit anti-human Greatwall,"Burgess

(8) GMCL1 controls 53BP1 stability and modulates taxane sensitivityElifeJanuary 19, 2026Yuki Kito, Tania J González-Robles, Sharon Kaisari, Juhee Pae, Sheena Faye Garcia, Juliana Ortiz-Pacheco, Beatrix Ueberheide, Antonio Marzio, Gergely Róna, Michele Paganop53 (1:1000, Proteintech 10442–1-AP), pHistone H3 (D2C8) (Ser10, 1:1000, Cell Signaling Technology, #3377), Ubiquitin (p37) (1:1000, Cell Signaling Technology, 58395 S), USP28 (1:1,000, Proteintech 17707–1-AP),

(9) GMCL1 controls 53BP1 stability and modulates taxane sensitivityeLifeJanuary 19, 2026Yuki Kito, Tania J González-Robles, Sharon Kaisari, Juhee Pae, Sheena Faye Garcia, Juliana Ortiz-Pacheco, Beatrix Ueberheide, Antonio Marzio, Gergely Róna, Michele Paganop53 (1:1000, Proteintech 10442–1-AP), pHistone H3 (D2C8) (Ser10, 1:1000, Cell Signaling Technology, #3377), Ubiquitin (p37) (1:1000, Cell Signaling Technology, 58395 S), USP28 (1:1,000, Proteintech 17707–1-AP),

(10) The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer CellsAdvanced ScienceJanuary 1, 2026Danrui Cui, Ruirui Qu, Tianqi Li, Linchen Wang, Xiaoyu Chen, Shengpeng Shao, Xue Liang, Jun Xu, Yi Sun, Xiufang Xiong, et al.(Proteintech, 20920‐1‐AP), Cyclin E1 (Cell Signaling Technology, 20808), p‐H3 (Cell Signaling Technology, 3377), and p‐Aurora A/B/C (Cell Signaling Technology, 2914), Aurora A (Cell Signaling Technology, 14 475),

(11) Targeting high-risk MYC-overexpressed osteosarcoma with an Aurora kinase inhibitor:--results from a pilot umbrella trialnpj Precision OncologyDecember 10, 2025Kai Tian, Yafei Jiang, Mengxiong Sun, Yu Lv, Jean Fan, Caixia Sun, Yujia Zhu, Yingying Yu, Xiaoyan Qiang, Peng Peng, et al.ab213480), PD-L1 (Cell Signaling Technology, 13648), Phospho-Histone H3 (Cell Signaling Technology, 3377 s), Histone H3 (Abcam, ab1791-100), Aurora A (Cell Signaling Technology, 14475S), Aurora B (Cell Signaling Technology

(12) Injury-induced Neuregulin-EGFR signaling from muscle mobilizes stem cells for whole-body regeneration in acoelsDevelopmental BiologyDecember 1, 2025Brian Stevens, Riley Popp, Heather Valera, Kyle Krueger, Christian P. Petersen10 % Horse Serum and incubation with rabbit anti-phospho-ser10-Histone3 (Cell Signaling Technology #3377S) at 1:500, then goat anti-rabbit-HRP (1:500, ThermoFisher G21234) secondary antibody, followed by tyramide

(13) Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasisNature Cell BiologyNovember 24, 2025Alberto Minetti, Omid Omrani, Christiane Brenner, Feyza Cansiz, Shinya Imada, Jonas Rösler, Saleh Khawaled, Gabriele Allies, Sven W. Meckelmann, Nadja Gebert, et al.For immunohistochemistry (IHC) experiments, rabbit monoclonal anti-pH3 (Ser10) (Cell Signaling Technologies no. 3377) antibody was used at 1:500, followed by a biotin-conjugated donkey anti-rabbit secondary antibody (1:500,

(14) The blood–brain barrier regulates brain tumor growth through the SLC36 amino acid transporter Pathetic in DrosophilaPLOS BiologyNovember 18, 2025Qian Dong, Edel Alvarez-Ochoa, Phuong-Khanh Nguyen, Paul Orih, Natasha Fahey-Lozano, Hina Kosakamoto, Fumiaki Obata, Cyrille Alexandre, Louise Y. Cheng(1:100, Abcam, #ab197788), rat anti-Dpn (1:200; Abcam, 195172), rabbit anti-pH3 (1:200, Cell Signaling, 3377), mouse anti-Elav (1:50; DSHB, 9F8A9), rabbit anti-Dcp-1-cleaved (1:100, Cell Signaling, 9578S), and

(15) N2B27 media formulations influence gastruloid developmentDevelopmentNovember 15, 2025Tina Balayo, Sharna Lunn, Pau Pascual-Mas, Ulla-Maj Fiuza, Amruta Vasudevan, Joshua D. Frenster, Joel B. Josende García, Hannah Y. Galloon, Raquel Flores Peirats, Alfonso Martinez Arias, et al.cells were stained with the primary antibodies anti-phospho-histone 3 (dilution 1:1600, Cell Signaling, 3377) and anti-cleaved-caspase 3 (dilution 1:5000, Cell Signaling, 9664). A goat-anti-rabbit Alexa-488-conjugated

(16) Benchmarking porcine pancreatic ductal organoids for drug screening applicationsEMBO Molecular MedicineNovember 4, 2025Christos Karampelias, Kaiyuan Yang, Falk J Farkas, Michael Sterr, Mireia Molina van Den Bosch, Simone Renner, Janina Fuß, Christine von Toerne, Sören Franzenburg, Tatsuya Kin, et al.Bank,F55A10 Sheep a-NEUROG3,R&D Systems,AF3444 Rabbit a-phospho (Ser10) histone 3,Cell Signaling,3377S Rabbit a-pancreatic amylase,Abcam,21156 Guinea pig a-INS,LSBio,LS-C85862-1 Guinea pig a-GCG,Takara,M182 Rabbit

(17) Squamate ventricular cardiomyocytes: Ploidy, proliferation, and heart muscle cell size in the leopard gecko ( Eublepharis macularius )Developmental DynamicsNovember 1, 2025Kathy Jacyniak, Karemna Barrera Jaimes, Minh Hanh Doan, Jordyn M. Chartrand, Matthew K. Vickaryousfor 30 min at 37°C,"Rabbit anti‐pHH3 1:500 in diluent overnight at 4°C (Cell Signaling Technology, 3377, AB_1549592)","Cy3 Goat anti‐Rabbit 1:250 in 1XPBS for 1 h at room temperature (Jackson ImmunoResearch

(18) DIAPH3 is upregulated in high-grade gliomas and linked to chromosomal instabilityNeuro-Oncology AdvancesOctober 24, 2025Caren Jabbour, Mathilde Beka, Philippe Gailly, Nicolas Tajeddine(Abcam, #AB245660; 1:2500), rabbit polyclonal anti-phospho-Histone H3 (Cell Signaling Technology, #3377; 1:1000), and rabbit monoclonal anti-GAPDH (Cell Signaling Technology, #2118; 1:10 000). After washing,

(19) Planarian microtubules form a network within muscle and regulate injury-induced genes essential for regeneration patterningDevelopmentOctober 15, 2025Xavier N. Anderson, Christian P. Petersenincubated overnight at room temperature with rabbit anti-ser10-H3P antibody (Cell Signaling Technology, 3377S) at 1:300 diluted in blocking solution. Animals were washed with PBSTx six times over 6 h prior to incubation

(20) CAF-Driven Mechanotransduction via Collagen Remodeling Accelerates Tumor Cell Cycle ProgressionGelsAugust 13, 2025Yating Xiao, Yingying Jiang, Ting Bao, Xin Hu, Xiang Wang, Xiaoning Han, Linhong Dengstained with KI67 (Abcam, Cambridge, UK, ab15580, 1:500) or Phospho-Histone H3 (CST, Danvers, MA, USA, 3377, 1:500) and secondary AF488 antibody (Thermo, Waltham, MA, USA, A-11008, 1:500). FUCCI cell-cycle phases

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Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb

Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb from Cell Signaling Technology

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