Fig 2: Box plots of the PA1-nuc H-score w/ vs. w/o breast cancer relapse in luminal A type breast cancer.There was significant difference between relapse group (median [25%, 75%]; 82 [20,103]) and no relapse one (101 [70,150]) in the Mann Whitney U test (P = 0.032). Abbreviations: PA1-nuc, nuclear PA1 expression; PA1-cyto, cytoplasmic PA1 expression.

Fig 3: Representative microscopic views of PA1 staining with rabbit polyclonal antibody against PA1.a, nuclear staining; b, cytoplasmic staining; c, mixed nuclear and cytoplasmic staining; d, negative staining (original magnification × 400) e, histograms for Histo-Score (HS) of PA1-nuc and f, of PA1-cyto (mean; 51.4, S.D.; 62.4). Abbreviations: PA1-nuc, nuclear PA1 expression; PA1-cyto, cytoplasmic PA1 expression; S.D., Standard Deviation.

Fig 4: Kaplan–Meier plots of the association of PA1 expression in the nucleus with RFS; A and BCSS; B in the entire cohort.When PA1 protein expression was defined as either positive or negative, positive cases seemed to have a longer survival than negative ones in Kaplan–Meier method analysis (RFS, P = 0.023), but no slightly correlation with BCSS (P = 0.23). No association could be found between PA1 protein expression in the cytoplasm and RFS, BCSS (data not shown). Abbreviations: RFS, relapse-free survival; BCSS, breast cancer-specific survival.