PERK (C33E10) Rabbit mAb 3192S from Cell Signaling Technology

Product Specs
ItemPERK (C33E10) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3192S
Quantity100 µl
ApplicationsW, W-S
ReactivityH, M, R, Mk
TargetPERK
HostRabbit
NCBI Gene AliasesPEK, PERK, WRS
ClonalityMonoclonal
NCBI Full Gene Nameeukaryotic translation initiation factor 2 alpha kinase 3
Research AreaProtein Folding and Trafficking
IsotypeIgG
CloneC33E10
Molecular Weight140

Questions and Answers

Can this antibody be used to study the integrated stress response in human cells?

Yes, the PERK (C33E10) Rabbit mAb has been utilized in studies investigating the integrated stress response, particularly in the context of eIF2α phosphorylation and its role in cellular stress responses. For instance, it has been referenced in research examining how 24(S)-hydroxycholesterol induces stress responses that lead to unconventional cell death, highlighting its relevance in understanding the mechanisms of the unfolded protein response (Urano et al., 2022).

What species can this antibody detect, and is it effective for human samples?

The PERK (C33E10) Rabbit mAb is reactive with multiple species, including humans (Homo sapiens), mice (Mus musculus), rats (Rattus norvegicus), and non-human primates. This broad reactivity makes it suitable for comparative studies across different model organisms.

How has this antibody been applied in Western blot experiments?

The PERK antibody has been shown to be effective in Western blot applications, as evidenced by its use in various studies to detect total PERK protein levels. For example, it has been cited in protocols where it was used at a dilution of 1:1000 to analyze protein expression in response to stress conditions (Belyy et al., 2022; Bryk et al., 2020).

Are there any specific experimental contexts where this antibody has been particularly useful?

This antibody has been particularly useful in studies related to endoplasmic reticulum stress and the unfolded protein response. It has been cited in research exploring the activation of PERK signaling pathways in various cellular contexts, including cancer biology and metabolic disorders, indicating its importance in understanding stress-related cellular mechanisms (Panda et al., 2022; Urano et al., 2019).

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Neuronal SEL1L-HRD1 ER-associated degradation is essential for motor function and survival in miceJournal of Clinical InvestigationApril 15, 2026Mauricio Torres, You Lu, Brent Pederson, Hui Wang, Anna Gretzinger, Liangguang L. Lin, Jiwon Hwang, Xinxin Chen, Alan C. Rupp, Abigail J. Tomlinson, et al.1:5,000), anti-IRE1α (Cell Signaling Technology, 3294, 1:2,000), anti-PERK (Cell Signaling Technology, 3192, 1:1,000), anti–p-eIF2α (Cell Signaling Technology, 9721, 1:1,000), anti-eIF2α (Cell Signaling Technology,

(2) Saikosaponin D Is Associated with Anti-Tumor Effects and Markers of Autophagy and Endoplasmic Reticulum Stress in Human Endometrial Cancer Ishikawa CellsNutrientsApril 13, 2026Xiu-Xiu Zhang, Tong-Tong Tang, Xiao-Mei Ma, Kiran Thakur, Fei Hu, Jian-Guo Zhang, Yi-Long Ma, Zhao-Jun Weip62/SQSTM1 (Cell Signaling Technology, #8025, 1:1000, Danvers, MA, USA), PERK (Cell Signaling Technology, #3192, 1:1000, Danvers, MA, USA), p-eIF2α (Cell Signaling Technology, #3398, 1:1000, Danvers, MA, USA), eIF2α

(3) Serglycin Cooperates with the Unfolded Protein Response Pathway and Inflammation to Drive Glioblastoma Cell SurvivalCellsApril 9, 2026Eleftherios N. Athanasopoulos, Chrysostomi Gialeli, Angeliki Natsiou, Dimitra Manou, Vassiliki T. Labropoulou, Achilleas D. Theocharis1:1000), phospho-PERK (Cell Signaling Inc., #3179, dilution 1:1000), total-PERK (Cell Signaling Inc., #3192, dilution 1:1000), phospho-p65 (Santa Cruz Biotechnology, Inc., #sc-136548, dilution 1:750), total-p65

(4) Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathwayNeural Regeneration ResearchApril 1, 2026Jie Jing, Shiling Chen, Xuan Wu, Jingfei Yang, Xia Liu, Jiahui Wang, Jingyi Wang, Yunjie Li, Ping Zhang, Zhouping TangAB_2876891), PRKR-like endoplasmic reticulum kinase (PERK; rabbit, 1:1000, Cell Signaling Technology, Cat# 3192S, RRID: AB_2095847), phospho-PERK (rabbit, 1:1000, Cell Signaling Technology, Cat# 3179S, RRID: AB_2095853),

(5) Free Fatty Acids Induce Endoplasmic Reticulum Stress-Mediated Apoptosis of Macrophages in Dairy Cows with KetosisAnimals (Basel)April 1, 2026Hongdou Jia, Xinyuan Sun, Wantong Cheng, Yue Yu, Yutong Wu, Jiayi Yan, Yunhui Fan, Qiushi Xu, Juan J. Loor, Chuang Xu, et al.Proteintech, Wuhan, China), p-IRE1 (1:1000; 3294, Cell Signaling Technology, Danvers, MA, USA), PERK (1:1000; 3192, Cell Signaling Technology, Danvers, MA, USA), p-PERK (1:1000; 3179, Cell Signaling Technology, Danvers,

(6) ENDOU-1-induced cytoplasmic HnRNPA3 recognizes m6A methylation on the upstream reading frame of human CHOP transcripts to achieve maximal CHOP translationCellular and Molecular Life SciencesMarch 28, 2026Hung-Chieh Lee, Yi-Hsin Huang, Chi-Cheng Hsieh, Yi-Nan Ke, Huai-Jen TsaiProteintech, 10835-1-AP), eIF2α (1:1,000; CST, #5324); p-eIF2α (1:1,000; CST, #3398); PERK (1:1,000; CST, #3192); p‐PERK (1:1,000; Abcam, Ab156919); Bip (1:1,000; Proteintech, 11587-1-AP); Flag (1:20,000; Proteintech,

(7) Acid Ceramidase Inhibition Disrupts Ceramide Homeostasis and Induces Mitochondrial Apoptosis in IDH1-Mutant OligodendrogliomaResearch SquareMarch 25, 2026Mioara Larion, Helena Muley, Faris Zaibaq, Meili Zhang, Dionne Davis, Michael Kruhlak, Samarth Mathur, Adrian Lita, Hua Song, Wei Zhang, et al.anti-AC (HPA005468 Sigma-Aldrich), mouse anti-IDH1 (R132H) (SAB4200548 Sigma-Aldrich), rabbit anti-PERK (3192S Cell Signaling), rabbit anti-ATF4 (11815S Cell Signaling), rabbit anti-Caspase 8 (13423–1-AP Proteintech),

(8) MSMO1 promotes chemotherapy resistance through modulation of T-MAS metabolism via PERK/elF2α/ATF4/CHOP pathwayiScienceMarch 20, 2026Hengyu Ren, Xuliren Wang, Zhibo Shao, Min Xiong, Weiru Chi, Yuting Sang, Qi Zhang, Zehao Wang, Douwaner Liu, Liyi Zhang, et al.,Cell Signaling Technology,Cat# 3398; RRID:AB_2096481 Rabbit monoclonal PERK antibody,Cell Signaling Technology,Cat# 3192; RRID:AB_2095847 Rabbit polyclonal CHOP antibody,Proteintech,Cat# 15204-1-AP; RRID:AB_2292610 ,, Biological

(9) Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed HepatocytesInt J Mol SciMarch 19, 2026Olaya Yassin, Odai Darawshi, Fangfang Wang, Youwei Zhang, Ata Abbas, William C. Merrick, William Cheung, Antony Antoniou, Shakti P. Pattanayak, Boaz Tiroshused as the chemiluminescent substrate. The antibodies that were used are listed here: anti-PERK (CST #3192), anti-IRE1 (CST #3294), anti-pAKT (CST #13038), anti-PTEN (CST #9188), anti-Puromycin (Millipore Sigma,

(10) ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradationThe EMBO JournalMarch 12, 2026Akruti Shah, Aaztli R Coria, Britnie Santiago Membréno, Emilien Orgebin, Jennifer T Miller, Wilfried Guiblet, Colin Chih-Chien Wu(Enzo, ADI-SPA-860), calreticulin (Novus Biologicals, NB600-101), and PERK (Cell Signaling Technology, 3192). Flow cytometric CRISPR screen Lentivirus was generated from the Brunello human CRISPR knockout pooled

(11) Amino acid restriction sensitizes lung cancer cells to ferroptosis via GCN2-dependent activation of the integrated stress responseRedox BiologyMarch 1, 2026Viktor Antonsson Garellick, Nadia Gul, Parvin Horrieh, Dyar Mustafa, Angana A.H. Patel, Martin Dankis, Samantha W. Alvarez, Johanna Berndtson, Saeed Mahdavi, Maria Schwarz, et al.S6 (2217; CST), TfrC (13–6800; Invitrogen), p-PKR (ab32036; Abcam), p-PERK (ab192591; Abcam), PERK (3192; CST), PKR (sc-6282; SCBT), and Hsp90 (4877; CST). Secondary antibody used was peroxidase-conjugated

(12) SEL1L-HRD1 ER-associated degradation facilitates prohormone convertase 2 maturation and glucagon production in islet α cellsNature CommunicationsFebruary 25, 2026Wenzhen Zhu, Linxiu Pan, Xianwei Cui, Anna Chiara Russo, Rohit Ray, Brent Pederson, Xiaoqiong Wei, Liangguang Leo Lin, Mauricio Torres, Hannah Hafner, et al.(Abcam ab21685, 1:5,000), anti-IRE1α (Cell Signaling Technology [CST] 3294, 1:2,000), anti-PERK (CST 3192, 1:1000), anti-eIF2α (CST 9722, 1:1000), anti-phospho-eIF2α (CST 9721, 1:1000), anti-PC2 (CST 14013

(13) The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2α pathwayVeterinary ResearchFebruary 25, 2026Jingwen Dai, Pingping Zhou, Yanjin Wang, Haojie Ren, Kehui Zhang, Meilin Li, Hailiang Ge, Hongwei Cao, Jiaqi Li, Hao Deng, et al.ab214714, Abcam), rabbit anti-BiP PAbs (catalog no. A0241, ABclonal), mouse anti-PERK MAb (catalog no. 3192S, CST), rabbit anti-p-PERK PAbs (catalog no. BS66100, Bioworld), rabbit anti-p-eIF2α PAbs (catalog no.

(14) Encephalopathy-linked UFM1 variants impede neuronal protein translation, development, and functionEMBO Molecular MedicineFebruary 23, 2026Catarina Perdigão, Josefa Torres, Helge M Magnussen, Janina Koch, Elena Rudashevskaya, Frederieke Moschref, Maksims Fiosins, Fritz Benseler, Sally Wenger, Tanja Nilsson, et al.(1:500 ICC),NeuroMab,75-028 RFP (1:1000 ICC),Synaptic Systems,390 004 PERK (1:1000 WB),Cell Signaling,3192 ATF6 (1:1000 WB),Novus Biologicals,NBP1-40256 IRE1α (1:1000 WB),Cell Signaling,3294 p-eIF2α (Ser51)

(15) Shear stress-induced Ca2+ influx triggers endoplasmic reticulum stress and cardiomyocyte apoptosis: implications for mitral regulationBiological ResearchFebruary 8, 2026Wei-Ting Chang, Jun-Ming Su, Fan-E Mo, Jhih-Yuan Shih, Wen-Tai ChiuGrp78 (BD, 610978), ATF6 (Santa Cruz, sc166659), p-PERK (Cell Signaling, 3179), PERK (Cell Signaling, 3192), p-IRE1α (Abcam, ab48187), IRE1α (Abcam, ab37073), p-eIF2α (Cell Signaling, 9721), eIF2α (Cell Signaling,

(16) In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogenNature CommunicationsFebruary 3, 2026Alexandre Giraud-Gatineau, Georges Haustant, Marc Monot, Mathieu Picardeau, Nadia Benaroudj(5174; Cell Signaling), anti-MLC2 (8505; Cell Signaling), anti-pMLC2 (3671; Cell Signaling), anti-PERK (3192; Cell Signaling), anti-pPERK (PA5-40294; ThermoFisher), anti-IRE1 (3294; Cell Signaling), anti-pIRE1

(17) The endoplasmic reticulum displays high polarity with low protein aggregation in human cellsCommun BiolFebruary 3, 2026Xinwei Hu, Junlin Chen, Ping Liu, Huaiyue Zhang, Yu Liu, Xin Zhang, Lei Wang(Abcam, ab124945), anti-IRE1α (Cell Signaling Technology, #3294S), anti-PERK (Cell Signaling Technology, #3192S), anti-pS51-eIF2α (Cell Signaling Technology, #9721S), anti-eIF2α (Cell Signaling Technology, #5324S),

(18) Zinc accumulation-induced integrated stress response triggers β-cell identity lossCell ResearchJanuary 28, 2026Qing Ma, Wenjun Xu, Xuan Wang, Haoyu Nie, Yukun Gao, Rui Hu, Zhihao Yang, Xushu Wang, Ta Na, Xiangyi Chen, et al.#sc-7819, 1:100), anti-ATF4 (Proteintech, #10835-1-AP, 1:1000), anti-PERK (Cell Signaling Technology, #3192S, 1:1000), anti-p-PERK (ABclonal, #AP1501, 1:1000), anti-eIF2α (Beyotime, #AF6771, 1:1000), anti-p-eIF2α

(19) Stenotrophomonas promotes gastrointestinal tumor progression via STING degradation in tumour cells and mitigated immune responseNature CommunicationsJanuary 26, 2026Huolun Feng, Kunling Chen, Zhihui Xi, Baijin Xia, Xi Chen, Yiqin Huang, Zuda Huang, Jieqing Guo, Dandan Zhu, Fa Ling, et al.29801-1-AP, Proteintech), anti-Phospho-PERK (cat: 3179 T, Cell Signaling Technology), anti-PERK (cat: 3192 T, Cell Signaling Technology), anti-cGAS (cat:79978, Cell Signaling Technology), anti-IRE1α (cat: 3294,

(20) The critical role of GRP78/BiP MARylation in ER stress of KRAS-mutant colorectal cancerJCI InsightJanuary 23, 2026Shuxian Zhang, Xiaodan Chen, Qian Gong, Jing Huang, Yi Tang, Ming Xiao, Ming Li, Qingshu Li, Yalan WangSigma-Aldrich (catalog I9890) for inhibiting ART1. The following antibodies were used: ATF6 (catalog 3192), PERK (catalog 65880), IRE1α (catalog 3294), eIF2α (catalog 5324), p-eIF2α (catalog 3398) (all Cell Signaling Technology

Be the First to Write a Review!

PERK (C33E10) Rabbit mAb

PERK (C33E10) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (557)

Reviews

PERK (C33E10) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map