A: Indirect immunohystochemistry of a mouse striatum section (dilution 1 : 100).
B: Indirect immunohystochemistry of a mouse hippocampus section (dilution 1 : 100).
ChT positive structures were revealed with a biotinylated goat anti-mouse secondary antibody in combination with peroxidase coupled streptavidin. Nickle amplified diaminobenzidine was used as chromagen.

Courtesy: W. Härtig, Paul Flechsig Institute, Leipzig

Indirect immunostaining of PFA fixed paraffin embedded mouse spinal cord section with mouse anti-ChT (cat. no. 216 011, dilution 1 : 1000, DAB). Nuclei have been visualized by haematoxylin staining (blue).​