Fig 1: SLMAP3 impacts protein stability of SIKE1 and FGFR1OP2. (a) Loss of SLMAP3 does not affect the protein levels of STRIP1, Strn and the catalytic subunit of PP2A (PP2A-C) (n = 3). (b) Knockout of SLMAP3 leads to reduced protein levels of SIKE1 in MEF (n = 3), (c) whole embryos (n = 3) and (d) in multiple tissue. (e) The protein levels of FGFR1OP2 is also reduced in SLMAP3-/- MEFs (n = 3). (f) Transcripts of SIKE1 and FGFR1OP2, assessed by RT-qPCR, are not significantly altered in cells deficient of SLMAP (n = 3). (g) Treatment of SLMAP3-/- MEFs with MG132 was enough to increase SIKE1 expression after 24 h. (h) The knockout of FGFR1OP2 reduced protein levels of both SLMAP3 and SIKE1, but (i) did not change their transcripts, which was assessed by RT-qPCR (n = 3). (j) Treatment of MEFs deficient in FGFROP2 with MG132 increased the protein levels of SIKE1 but not SLMAP3. (k) The protein levels of SLMAP3 did not increase even with NH4Cl treatment. *p < 0.05, **p < 0.01, ***p < 0.001.