Fig 1: IP–MS identifies that LUZP1 is one of the top interacting proteins of DAPK3. A) Schematic representation of IP–MS workflow using a DAPK3 antibody in SUM159 control and DAPK3 KO #3 cells. B) Top 10 MF terms of GO overrepresentation analysis with a total of 73 DAPK3-interacting proteins identified from IP–MS analysis. P-values are determined by the overrepresentation analysis. C) Top 5 interacting proteins of DAPK3 identified from IP–MS analysis. The proteins are ranked based on the ratio of iBAQ levels in DAPK3 KO #3 cells compared with control cells. The full list of proteins in the DAPK3 immunoprecipitants is shown in Table S1. D) Immunoblotting shows the protein levels of LUZP1 and DAPK3 in whole-cell lysates and co-IP samples of anti-DAPK3 antibody obtained from SUM159 control and DAPK3 KO #3 cells. The representative image is based on two independent experiments. E) Immunoblotting shows the protein levels of LUZP1 and DAPK3 in whole-cell lysates and co-IP samples of antiLUZP1 antibody obtained from SUM159 control and LUZP1 KO #6 cells. The representative image is based on two independent experiments. F) Immunoblotting shows the protein levels of LUZP1 and HA-tagged DAPK3 in whole-cell lysates and co-IP samples of anti-HA antibody obtained from SUM159 DAPK3 KO #3 cell, DAPK3 KO #3 cells with HA-tagged DAPK3 WT overexpression, and DAPK3 KO #3 cells with HA-tagged DAPK3 ΔLZ overexpression. The representative image is based on two independent experiments.