MyBioSource.com's Calpain I, II (small subunit) is a Mouse monoclonal antibody. The Calpain I, II (small subunit) Antibody was generated using Calpain 2, CAPN2, and mCANP as the antigen. It reacts with Human, and Bovine. This antibody has been shown to work in applications such as: ELISA, Immunoprecipitation, and Western Blot.
Description
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, -calpain is active at micromolar (M) concentrations of Ca ++. In addition to the ubiquitously expressed m- and calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux